File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Appears in Collections:
Conference Paper: The effect of tumor derived HMBG1 on intra-tumoral B cells in esophageal squamous cell carcinoma
Title | The effect of tumor derived HMBG1 on intra-tumoral B cells in esophageal squamous cell carcinoma |
---|---|
Authors | |
Issue Date | 2018 |
Publisher | Oxford University Press. The Journal's web site is located at http://annonc.oxfordjournals.org/ |
Citation | European Society for Medical Oncology (ESMO) Immuno-Oncology Congress 2018, Geneva, Switzerland, 13-16 December 2018. In Annals of Oncology, 2018, v. 29 n. Suppl.10, p. x7-x8 How to Cite? |
Abstract | Background: Tumor microenvironment (TME) is often hypoxic and characterized by diverse cell populations (tumor cells and lymphocytes). An intranuclear architectural protein termed high mobility group box chromosomal protein 1 (HMGB-1) is enriched in hypoxic environment, where it acts as a chemokine to promote recruitment of inflammatory cells. We studied the effect of tumor derived HMBG1 on B cell migration and phenotype differentiation in esophageal squamous cell carcinoma (ESCC).
Methods: Immunohistochemistry (IHC) staining for HMGB-1 was performed on tissue microarray (TMA) of ESCC and correlated with survival outcome. An immunostaining scoring system corresponding to total staining intensity as follows; (strong staining score =3; moderate staining score=2; weak staining scores= 1; no staining scores= 0). Co-staining with CD20+B was also performed to study B cells distribution in the tumor. ESCC cell lines were transfected with HMGB-1 and transwell migration of B cells were studied.
Results: TMA containing 78 paired primary tumors and normal tissue from ESCC patients was analyzed by IHC using an anti-HMGB1 antibody. We found that HMGB1 was expressed at a higher level as compared to paired normal tissue. Survival curves were estimated by the Kaplan-Meier method and compared with log-rank test to evaluate the relationship between biomarker HMGB1 and survival outcomes. Patients were divided into two groups based on the optimal cutoffs of low (score 0-1), high (score 2-3) staining of HMGB1, we found out that HMGB1 staining were not correlated to patient survivals (p = 0.363). We then performed double-staining and demonstrated that B cells were located in the stroma along HMGB1-stained tumor. Stable HMGB1 ESCC cell lines were established where boyden chamber and wound-healing assays demonstrated that B cells migrated and proliferated at higher rates towards HMGB1-overexpressing cell lines.
Conclusions: Our findings indicate that HMGB1 was over-expressed in tumor compared with normal tissue. HMGB1 expression per se was not correlated to survival in our log-rank analysis. HMGB1 expression has probable role in B cells recruitment, migration/proliferation. |
Description | Poster Session - No. 23P |
Persistent Identifier | http://hdl.handle.net/10722/275381 |
ISSN | 2023 Impact Factor: 56.7 2023 SCImago Journal Rankings: 13.942 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwong, DLW | - |
dc.contributor.author | Kam, YN | - |
dc.contributor.author | Guan, XY | - |
dc.date.accessioned | 2019-09-10T02:41:25Z | - |
dc.date.available | 2019-09-10T02:41:25Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | European Society for Medical Oncology (ESMO) Immuno-Oncology Congress 2018, Geneva, Switzerland, 13-16 December 2018. In Annals of Oncology, 2018, v. 29 n. Suppl.10, p. x7-x8 | - |
dc.identifier.issn | 0923-7534 | - |
dc.identifier.uri | http://hdl.handle.net/10722/275381 | - |
dc.description | Poster Session - No. 23P | - |
dc.description.abstract | Background: Tumor microenvironment (TME) is often hypoxic and characterized by diverse cell populations (tumor cells and lymphocytes). An intranuclear architectural protein termed high mobility group box chromosomal protein 1 (HMGB-1) is enriched in hypoxic environment, where it acts as a chemokine to promote recruitment of inflammatory cells. We studied the effect of tumor derived HMBG1 on B cell migration and phenotype differentiation in esophageal squamous cell carcinoma (ESCC). Methods: Immunohistochemistry (IHC) staining for HMGB-1 was performed on tissue microarray (TMA) of ESCC and correlated with survival outcome. An immunostaining scoring system corresponding to total staining intensity as follows; (strong staining score =3; moderate staining score=2; weak staining scores= 1; no staining scores= 0). Co-staining with CD20+B was also performed to study B cells distribution in the tumor. ESCC cell lines were transfected with HMGB-1 and transwell migration of B cells were studied. Results: TMA containing 78 paired primary tumors and normal tissue from ESCC patients was analyzed by IHC using an anti-HMGB1 antibody. We found that HMGB1 was expressed at a higher level as compared to paired normal tissue. Survival curves were estimated by the Kaplan-Meier method and compared with log-rank test to evaluate the relationship between biomarker HMGB1 and survival outcomes. Patients were divided into two groups based on the optimal cutoffs of low (score 0-1), high (score 2-3) staining of HMGB1, we found out that HMGB1 staining were not correlated to patient survivals (p = 0.363). We then performed double-staining and demonstrated that B cells were located in the stroma along HMGB1-stained tumor. Stable HMGB1 ESCC cell lines were established where boyden chamber and wound-healing assays demonstrated that B cells migrated and proliferated at higher rates towards HMGB1-overexpressing cell lines. Conclusions: Our findings indicate that HMGB1 was over-expressed in tumor compared with normal tissue. HMGB1 expression per se was not correlated to survival in our log-rank analysis. HMGB1 expression has probable role in B cells recruitment, migration/proliferation. | - |
dc.language | eng | - |
dc.publisher | Oxford University Press. The Journal's web site is located at http://annonc.oxfordjournals.org/ | - |
dc.relation.ispartof | Annals of Oncology | - |
dc.relation.ispartof | ESMO Immuno-Oncology Congress 2018 | - |
dc.rights | Pre-print: Journal Title] ©: [year] [owner as specified on the article] Published by Oxford University Press [on behalf of xxxxxx]. All rights reserved. Pre-print (Once an article is published, preprint notice should be amended to): This is an electronic version of an article published in [include the complete citation information for the final version of the Article as published in the print edition of the Journal.] Post-print: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in [insert journal title] following peer review. The definitive publisher-authenticated version [insert complete citation information here] is available online at: xxxxxxx [insert URL that the author will receive upon publication here]. | - |
dc.title | The effect of tumor derived HMBG1 on intra-tumoral B cells in esophageal squamous cell carcinoma | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Kwong, DLW: dlwkwong@hku.hk | - |
dc.identifier.email | Guan, XY: xyguan@.hku.hk | - |
dc.identifier.authority | Kwong, DLW=rp00414 | - |
dc.identifier.authority | Guan, XY=rp00454 | - |
dc.identifier.doi | 10.1093/annonc/mdy493.021 | - |
dc.identifier.hkuros | 303431 | - |
dc.identifier.hkuros | 308054 | - |
dc.identifier.volume | 29 | - |
dc.identifier.issue | Suppl.10 | - |
dc.identifier.spage | x7 | - |
dc.identifier.epage | x8 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 0923-7534 | - |