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- Publisher Website: 10.1016/j.jcv.2018.10.004
- Scopus: eid_2-s2.0-85055289253
- PMID: 30368218
- WOS: WOS:000453568100003
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Article: HPV genotyping and E6/E7 transcript assays for cervical lesion detection in an Asian screening population—Cobas and Aptima HPV tests
Title | HPV genotyping and E6/E7 transcript assays for cervical lesion detection in an Asian screening population—Cobas and Aptima HPV tests |
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Authors | |
Keywords | Human papillomavirus Aptima HPV Cobas HPV Cervical cancer |
Issue Date | 2018 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv |
Citation | Journal of Clinical Virology, 2018, v. 109, p. 13-18 How to Cite? |
Abstract | Background:
High-risk human papillomavirus (hrHPV) detection and genotyping by Cobas HPV test has become an important technical platform in cervical cancer screening. It may be used as a co-test with cervical cytology or as a standalone test. Aptima HPV assay (AHPV) is another hrHPV test detecting 13 genotypes through qPCR based amplification of viral E6/E7 transcripts. Partial genotyping with Aptima HPV 16 18/45 genotype assay (AHPV GT) on positive samples is possible. Evidence supporting the performance of AHPV in Asian populations is scarce.
Objective:
To compare the performances of Cobas and AHPV in detection of cervical squamous intraepithelial lesions (SIL) and triage of cytologically equivocal smears in a cohort of Hong Kong women.
Study design:
442 liquid based cytology (LBC) residues with biopsy confirmed diagnoses were evaluated by both AHPV and Cobas HPV tests.
Results:
Overall, there was a moderate agreement between AHPV and Cobas (κ = 0.5082, 95% CI: 0.492–0.672). The sensitivities of AHPV and Cobas for detecting biopsy confirmed HSIL or worse lesions (HSIL+) were 96.71% (95% CI: 92.49%–98.92%) and 97.37% (95% CI: 93.40%–99.28%) respectively. AHPV demonstrated significantly higher specificity than Cobas (37.85% vs 23.96%, p < 0.0001). Both tests could identify all ASC-US and AGC cases with HSIL + in follow-up biopsies, but AHPV showed a significantly higher specificity in both settings (ASC-US: 28.81% vs 11.86%, p < 0.0001; AGC: 55.00% vs 20.00%, p = 0.0233).
Conclusions:
Both AHPV and Cobas were equally sensitive in detecting high-grade SIL in both scenarios of screening and ASC-US or AGC triage but AHPV showed a higher specificity. |
Persistent Identifier | http://hdl.handle.net/10722/275189 |
ISSN | 2023 Impact Factor: 4.0 2023 SCImago Journal Rankings: 1.344 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wong, OGW | - |
dc.contributor.author | Tsun, OKL | - |
dc.contributor.author | Tsui, EY | - |
dc.contributor.author | Chow, JNK | - |
dc.contributor.author | Ip, PCP | - |
dc.contributor.author | Cheung, ANY | - |
dc.date.accessioned | 2019-09-10T02:37:24Z | - |
dc.date.available | 2019-09-10T02:37:24Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Journal of Clinical Virology, 2018, v. 109, p. 13-18 | - |
dc.identifier.issn | 1386-6532 | - |
dc.identifier.uri | http://hdl.handle.net/10722/275189 | - |
dc.description.abstract | Background: High-risk human papillomavirus (hrHPV) detection and genotyping by Cobas HPV test has become an important technical platform in cervical cancer screening. It may be used as a co-test with cervical cytology or as a standalone test. Aptima HPV assay (AHPV) is another hrHPV test detecting 13 genotypes through qPCR based amplification of viral E6/E7 transcripts. Partial genotyping with Aptima HPV 16 18/45 genotype assay (AHPV GT) on positive samples is possible. Evidence supporting the performance of AHPV in Asian populations is scarce. Objective: To compare the performances of Cobas and AHPV in detection of cervical squamous intraepithelial lesions (SIL) and triage of cytologically equivocal smears in a cohort of Hong Kong women. Study design: 442 liquid based cytology (LBC) residues with biopsy confirmed diagnoses were evaluated by both AHPV and Cobas HPV tests. Results: Overall, there was a moderate agreement between AHPV and Cobas (κ = 0.5082, 95% CI: 0.492–0.672). The sensitivities of AHPV and Cobas for detecting biopsy confirmed HSIL or worse lesions (HSIL+) were 96.71% (95% CI: 92.49%–98.92%) and 97.37% (95% CI: 93.40%–99.28%) respectively. AHPV demonstrated significantly higher specificity than Cobas (37.85% vs 23.96%, p < 0.0001). Both tests could identify all ASC-US and AGC cases with HSIL + in follow-up biopsies, but AHPV showed a significantly higher specificity in both settings (ASC-US: 28.81% vs 11.86%, p < 0.0001; AGC: 55.00% vs 20.00%, p = 0.0233). Conclusions: Both AHPV and Cobas were equally sensitive in detecting high-grade SIL in both scenarios of screening and ASC-US or AGC triage but AHPV showed a higher specificity. | - |
dc.language | eng | - |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv | - |
dc.relation.ispartof | Journal of Clinical Virology | - |
dc.subject | Human papillomavirus | - |
dc.subject | Aptima HPV | - |
dc.subject | Cobas HPV | - |
dc.subject | Cervical cancer | - |
dc.title | HPV genotyping and E6/E7 transcript assays for cervical lesion detection in an Asian screening population—Cobas and Aptima HPV tests | - |
dc.type | Article | - |
dc.identifier.email | Wong, OGW: wonggw@hkucc.hku.hk | - |
dc.identifier.email | Tsun, OKL: okltsun@hku.hk | - |
dc.identifier.email | Tsui, EY: eytsui@hkucc.hku.hk | - |
dc.identifier.email | Ip, PCP: philipip@hku.hk | - |
dc.identifier.email | Cheung, ANY: anycheun@hkucc.hku.hk | - |
dc.identifier.authority | Ip, PCP=rp01890 | - |
dc.identifier.authority | Cheung, ANY=rp00542 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.jcv.2018.10.004 | - |
dc.identifier.pmid | 30368218 | - |
dc.identifier.scopus | eid_2-s2.0-85055289253 | - |
dc.identifier.hkuros | 303030 | - |
dc.identifier.volume | 109 | - |
dc.identifier.spage | 13 | - |
dc.identifier.epage | 18 | - |
dc.identifier.isi | WOS:000453568100003 | - |
dc.publisher.place | Netherlands | - |
dc.identifier.issnl | 1386-6532 | - |