File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: In vivo electroporation of a codon-optimized BERopt DNA vaccine protects mice from pathogenic Mycobacterium tuberculosis aerosol challenge

TitleIn vivo electroporation of a codon-optimized BERopt DNA vaccine protects mice from pathogenic Mycobacterium tuberculosis aerosol challenge
Authors
KeywordsTuberculosis
DNA vaccine
BERopt
BCG
Mycobacterium tuberculosis
Issue Date2018
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/tube
Citation
Tuberculosis, 2018, v. 113, p. 65-75 How to Cite?
AbstractDNA vaccines have been extensively studied as preventative and therapeutic interventions for various infectious diseases such as tuberculosis, HIV/AIDS and influenza. Despite promising progresses made, improving the immunogenicity of DNA vaccine remains a technical challenge for clinical development. In this study, we investigated a tuberculosis DNA vaccine BERopt, which contained a codon-optimized fusion immunogen Ag85B-ESAT-6-Rv2660c for enhanced mammalian cell expression and immunogenicity. BERopt immunization through in vivo electroporation in BALB/c mice induced surprisingly high frequencies of Ag85B tetramer(+) CD8(+) T cells in peripheral blood and IFN-gamma-secreting CD8(+) T cells in splenocytes. Meanwhile, the BERopt vaccine-induced long-lasting T cell immunity protected BALB/c mice from high dose viral challenge using a modified vaccinia virus Tiantan strain expressing mature Ag85B protein (MVTT-m85B) and the virulent M. tb H37Rv aerosol challenge. Since the BERopt DNA vaccine does not induce anti-vector immunity, the strong immunogenicity and protective efficacy of this novel DNA vaccine warrant its future development for M. tb prevention and immunotherapy to alleviate the global TB burden.
Persistent Identifierhttp://hdl.handle.net/10722/274421
ISSN
2017 Impact Factor: 2.727
2015 SCImago Journal Rankings: 1.504
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTang, J-
dc.contributor.authorCai, Y-
dc.contributor.authorLiang, J-
dc.contributor.authorTan, Z-
dc.contributor.authorTang, X-
dc.contributor.authorZhang, C-
dc.contributor.authorCheng, L-
dc.contributor.authorZhou, J-
dc.contributor.authorYam, WC-
dc.contributor.authorChen, X-
dc.contributor.authorWang, H-
dc.contributor.authorWang, H-
dc.contributor.authorChen, Z-
dc.date.accessioned2019-08-18T15:01:24Z-
dc.date.available2019-08-18T15:01:24Z-
dc.date.issued2018-
dc.identifier.citationTuberculosis, 2018, v. 113, p. 65-75-
dc.identifier.issn1472-9792-
dc.identifier.urihttp://hdl.handle.net/10722/274421-
dc.description.abstractDNA vaccines have been extensively studied as preventative and therapeutic interventions for various infectious diseases such as tuberculosis, HIV/AIDS and influenza. Despite promising progresses made, improving the immunogenicity of DNA vaccine remains a technical challenge for clinical development. In this study, we investigated a tuberculosis DNA vaccine BERopt, which contained a codon-optimized fusion immunogen Ag85B-ESAT-6-Rv2660c for enhanced mammalian cell expression and immunogenicity. BERopt immunization through in vivo electroporation in BALB/c mice induced surprisingly high frequencies of Ag85B tetramer(+) CD8(+) T cells in peripheral blood and IFN-gamma-secreting CD8(+) T cells in splenocytes. Meanwhile, the BERopt vaccine-induced long-lasting T cell immunity protected BALB/c mice from high dose viral challenge using a modified vaccinia virus Tiantan strain expressing mature Ag85B protein (MVTT-m85B) and the virulent M. tb H37Rv aerosol challenge. Since the BERopt DNA vaccine does not induce anti-vector immunity, the strong immunogenicity and protective efficacy of this novel DNA vaccine warrant its future development for M. tb prevention and immunotherapy to alleviate the global TB burden.-
dc.languageeng-
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/tube-
dc.relation.ispartofTuberculosis-
dc.subjectTuberculosis-
dc.subjectDNA vaccine-
dc.subjectBERopt-
dc.subjectBCG-
dc.subjectMycobacterium tuberculosis-
dc.titleIn vivo electroporation of a codon-optimized BERopt DNA vaccine protects mice from pathogenic Mycobacterium tuberculosis aerosol challenge-
dc.typeArticle-
dc.identifier.emailTan, Z: zwtan@hku.hk-
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hk-
dc.identifier.emailChen, Z: zchenai@hku.hk-
dc.identifier.authorityYam, WC=rp00313-
dc.identifier.authorityChen, Z=rp00243-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.tube.2018.07.003-
dc.identifier.pmid30514515-
dc.identifier.hkuros301454-
dc.identifier.volume113-
dc.identifier.spage65-
dc.identifier.epage75-
dc.identifier.isiWOS:000451766300009-
dc.publisher.placeUnited Kingdom-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats