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Article: Recognition of proteins by metal-chelation based fluorescent probes in cells

TitleRecognition of proteins by metal-chelation based fluorescent probes in cells
Authors
KeywordsHis-tag
metallomics
metalloproteomics
molecular imaging
thiol-reaction
Issue Date2019
PublisherFrontiers Media SA. The Journal's web site is located at http://www.frontiersin.org/chemistry
Citation
Frontiers in Chemistry, 2019 How to Cite?
AbstractFluorescent probes such as thiol-reactive and Ni2+-nitrilotriacetate (NTA) based probes provide a powerful toolbox for real-time visualization of a protein and a proteome in living cells. Herein, we first went through basic principles and applications of thiol-reactive based probes in protein imaging and recognition. We then summarize a family of metal-NTA based fluorescence probes in the visualization of His(6)-tagged protein and identification of metalloproteins at proteome-wide scale. The pros and cons of the probes, as well as ways to optimize them, are discussed.
Persistent Identifierhttp://hdl.handle.net/10722/273841
ISSN
2019 Impact Factor: 3.693
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorJiang, N-
dc.contributor.authorLi, H-
dc.contributor.authorSun, H-
dc.date.accessioned2019-08-18T14:49:39Z-
dc.date.available2019-08-18T14:49:39Z-
dc.date.issued2019-
dc.identifier.citationFrontiers in Chemistry, 2019-
dc.identifier.issn2296-2646-
dc.identifier.urihttp://hdl.handle.net/10722/273841-
dc.description.abstractFluorescent probes such as thiol-reactive and Ni2+-nitrilotriacetate (NTA) based probes provide a powerful toolbox for real-time visualization of a protein and a proteome in living cells. Herein, we first went through basic principles and applications of thiol-reactive based probes in protein imaging and recognition. We then summarize a family of metal-NTA based fluorescence probes in the visualization of His(6)-tagged protein and identification of metalloproteins at proteome-wide scale. The pros and cons of the probes, as well as ways to optimize them, are discussed.-
dc.languageeng-
dc.publisherFrontiers Media SA. The Journal's web site is located at http://www.frontiersin.org/chemistry-
dc.relation.ispartofFrontiers in Chemistry-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.subjectHis-tag-
dc.subjectmetallomics-
dc.subjectmetalloproteomics-
dc.subjectmolecular imaging-
dc.subjectthiol-reaction-
dc.titleRecognition of proteins by metal-chelation based fluorescent probes in cells-
dc.typeArticle-
dc.identifier.emailLi, H: hylichem@hku.hk-
dc.identifier.emailSun, H: hsun@hku.hk-
dc.identifier.authoritySun, H=rp00777-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fchem.2019.00560-
dc.identifier.pmid31448265-
dc.identifier.hkuros301440-
dc.identifier.isiWOS:000480247000001-
dc.publisher.placeSwitzerland-

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