Cytocompatibility of an experimental hydraulic tricalcium silicate cement

Abstract

Objectives: To investigate the cytocompatibility of a new hydraulic tricalcium silicate material in a three-dimensional cell culture model using human periodontal ligament stem cells (hPDLSC). Methods: Root canals of human mandibular premolars were prepared to an apical size of 80 and filled with one of the following materials (n=10): MTA Angelus (MTA) and an experimental material (Experimental Neo-ES/NES). A three-dimensional cell culture model was fabricated. The effect of these materials on hPDLSC was tested using Alizarin Red staining, Alkaline Phosphatase assay, cell proliferation tests, quantitative real-time PCR for gene expression analysis and ELISA to determine the effect of the materials on inflammatory markers. Statistical analysis of the data was performed with appropriate tests (p=0.05). Results: Experimental Neo-ES demonstrated significantly better biocompatibility than MTA Angelus in terms of mineralization, cell proliferation and inflammatory marker expression (p<0.05) Conclusions: Material composition plays an important role in its biological properties. Despite both being hydraulic tricalcium silicates, the experimental material (Neo-ES) showed better cytocompatibility to periodontal ligament stem cells, compared to the commercially available material.