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Conference Paper: IL-17 enhances osteogenic differentiation of MSCs in 3D-printed PCL scaffold
Title | IL-17 enhances osteogenic differentiation of MSCs in 3D-printed PCL scaffold |
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Authors | |
Issue Date | 2019 |
Publisher | International Association for Dental Research. The Journal's web site is located at http://www.iadr.org/ |
Citation | The 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec Iss A, abstract no. 3595 How to Cite? |
Abstract | Objectives: Interleukin (IL)-17 has been found to play an essential role in bone remodeling, and we have proved that IL-17 regulates the osteogenesis of mesenchymal stem cells (MSCs) trough osteocyte-specific pathways. Polycaprolactone (PCL) is a biodegradable polyester material approved by the FDA for its use in implants, drug delivery devices and tissue engineering. This study aimed to employ a 3D-printed PCL scaffold exploited for bone tissue engineering and use Transwell assays with osteocytes co-culture to examine whether the PCL scaffold could further enhance osteogenic differentiation and mineralization.
Methods: Mouse MSCs purified from mouse long bones were cultured on 3D-printed PCL discs. The scaffolds with mMSCs were placed at the bottom chamber of Transwell plates and MLO-Y4 osteocytes seeded in the upper chambers were placed over scaffolds in osteogenic media. After 10 d of culture in osteogenic media containing IL-17, Alizarin red staining was used to analyze the appearance of mineralized nodules. Furthermore, we repeated the assay with human induced pluripotent stem cell-derived MSCs which were previously obtained with a clinically compliant protocol and successfully used in regeneration.
Results: Compared with the control, cultures grown on the 3D-printed PCL scaffold showed an obvious increase in mineralized nodule formation in both mouse and human MSCs as early as day 10. Additionally, IL-17 alone or co-culture with MLO-Y4 osteocytes enhanced osteogenesis in these cultures. Furthermore, the synergistically role of IL-17 and osteocytes could be found in the osteogenesis within the scaffold.
Conclusions: The present study confirms that the 3D culture on PCL scaffold enhances IL-17–stimulated osteogenesis of MSCs when co-cultured with MLO-Y4 osteocytes. These synergistic effects by using PCL 3D-scaffolds support the potential benefits of IL-17–induced MSCs in the treatment of bone loss diseases and in the induction of self-renewal and self-repair of damaged osseous tissue through tissue engineering. |
Description | Poster Session: 399 - Stem Cell Biology and Tissue Regeneration - no. 3595 |
Persistent Identifier | http://hdl.handle.net/10722/273025 |
DC Field | Value | Language |
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dc.contributor.author | Liao, C | - |
dc.contributor.author | Hua, Y | - |
dc.contributor.author | Zhang, C | - |
dc.contributor.author | Jin, L | - |
dc.contributor.author | Yang, Y | - |
dc.date.accessioned | 2019-08-06T09:21:08Z | - |
dc.date.available | 2019-08-06T09:21:08Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | The 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec Iss A, abstract no. 3595 | - |
dc.identifier.uri | http://hdl.handle.net/10722/273025 | - |
dc.description | Poster Session: 399 - Stem Cell Biology and Tissue Regeneration - no. 3595 | - |
dc.description.abstract | Objectives: Interleukin (IL)-17 has been found to play an essential role in bone remodeling, and we have proved that IL-17 regulates the osteogenesis of mesenchymal stem cells (MSCs) trough osteocyte-specific pathways. Polycaprolactone (PCL) is a biodegradable polyester material approved by the FDA for its use in implants, drug delivery devices and tissue engineering. This study aimed to employ a 3D-printed PCL scaffold exploited for bone tissue engineering and use Transwell assays with osteocytes co-culture to examine whether the PCL scaffold could further enhance osteogenic differentiation and mineralization. Methods: Mouse MSCs purified from mouse long bones were cultured on 3D-printed PCL discs. The scaffolds with mMSCs were placed at the bottom chamber of Transwell plates and MLO-Y4 osteocytes seeded in the upper chambers were placed over scaffolds in osteogenic media. After 10 d of culture in osteogenic media containing IL-17, Alizarin red staining was used to analyze the appearance of mineralized nodules. Furthermore, we repeated the assay with human induced pluripotent stem cell-derived MSCs which were previously obtained with a clinically compliant protocol and successfully used in regeneration. Results: Compared with the control, cultures grown on the 3D-printed PCL scaffold showed an obvious increase in mineralized nodule formation in both mouse and human MSCs as early as day 10. Additionally, IL-17 alone or co-culture with MLO-Y4 osteocytes enhanced osteogenesis in these cultures. Furthermore, the synergistically role of IL-17 and osteocytes could be found in the osteogenesis within the scaffold. Conclusions: The present study confirms that the 3D culture on PCL scaffold enhances IL-17–stimulated osteogenesis of MSCs when co-cultured with MLO-Y4 osteocytes. These synergistic effects by using PCL 3D-scaffolds support the potential benefits of IL-17–induced MSCs in the treatment of bone loss diseases and in the induction of self-renewal and self-repair of damaged osseous tissue through tissue engineering. | - |
dc.language | eng | - |
dc.publisher | International Association for Dental Research. The Journal's web site is located at http://www.iadr.org/ | - |
dc.relation.ispartof | Journal of Dental Research (Spec Issue) | - |
dc.relation.ispartof | IADR/AADR/CADR 2019 General Session & Exhibition | - |
dc.title | IL-17 enhances osteogenic differentiation of MSCs in 3D-printed PCL scaffold | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.email | Jin, L: ljjin@hkucc.hku.hk | - |
dc.identifier.email | Yang, Y: yangyanq@hku.hk | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.identifier.authority | Jin, L=rp00028 | - |
dc.identifier.authority | Yang, Y=rp00045 | - |
dc.identifier.hkuros | 299745 | - |
dc.identifier.volume | 98 | - |
dc.identifier.issue | Spec Iss A | - |
dc.identifier.spage | abstract no. 3595 | - |
dc.identifier.epage | abstract no. 3595 | - |
dc.publisher.place | United States | - |