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Article: Optimization of Protein–Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy

TitleOptimization of Protein–Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy
Authors
KeywordsProteins
Force
Substrates
Drugs
Spectroscopy
Issue Date2019
PublisherIEEE. The Journal's web site is located at http://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=7729
Citation
IEEE Transactions on Nanotechnology, 2019, v. 18, p. 509-517 How to Cite?
AbstractIncreasingly targeted in drug discovery, protein-protein interactions challenge current high throughput screening technologies in the pharmaceutical industry. Developing an effective and efficient method for screening small molecules or compounds is critical to accelerate the discovery of ligands for enzymes, receptors, and other pharmaceutical targets. Here, we report developments of methods to increase the signal-to-noise ratio for screening protein-protein interactions using atomic force microscopy (AFM) force spectroscopy. We have demonstrated the effectiveness of these developments on detecting the binding process between focal adhesion kinases with protein kinase B (Akt1), which is a target for potential cancer drugs. These developments include optimized probe and substrate functionalization processes and redesigned probe-substrate contact regimes. Furthermore, a statistical-based data processing method was developed to enhance the contrast of the experimental data. Collectively, these results demonstrate the potential of the AFM force spectroscopy in automating drug screening with high throughput.
Persistent Identifierhttp://hdl.handle.net/10722/272905
ISSN
2017 Impact Factor: 2.857
2015 SCImago Journal Rankings: 0.708

 

DC FieldValueLanguage
dc.contributor.authorYang, Y-
dc.contributor.authorZeng, B-
dc.contributor.authorSun, Z-
dc.contributor.authorEsfahani, AM-
dc.contributor.authorHou, J-
dc.contributor.authorJiao, ND-
dc.contributor.authorLiu, L-
dc.contributor.authorChen, L-
dc.contributor.authorYang, R-
dc.contributor.authorXi, N-
dc.date.accessioned2019-08-06T09:18:47Z-
dc.date.available2019-08-06T09:18:47Z-
dc.date.issued2019-
dc.identifier.citationIEEE Transactions on Nanotechnology, 2019, v. 18, p. 509-517-
dc.identifier.issn1536-125X-
dc.identifier.urihttp://hdl.handle.net/10722/272905-
dc.description.abstractIncreasingly targeted in drug discovery, protein-protein interactions challenge current high throughput screening technologies in the pharmaceutical industry. Developing an effective and efficient method for screening small molecules or compounds is critical to accelerate the discovery of ligands for enzymes, receptors, and other pharmaceutical targets. Here, we report developments of methods to increase the signal-to-noise ratio for screening protein-protein interactions using atomic force microscopy (AFM) force spectroscopy. We have demonstrated the effectiveness of these developments on detecting the binding process between focal adhesion kinases with protein kinase B (Akt1), which is a target for potential cancer drugs. These developments include optimized probe and substrate functionalization processes and redesigned probe-substrate contact regimes. Furthermore, a statistical-based data processing method was developed to enhance the contrast of the experimental data. Collectively, these results demonstrate the potential of the AFM force spectroscopy in automating drug screening with high throughput.-
dc.languageeng-
dc.publisherIEEE. The Journal's web site is located at http://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=7729-
dc.relation.ispartofIEEE Transactions on Nanotechnology-
dc.rightsIEEE Transactions on Nanotechnology. Copyright © IEEE.-
dc.rights©20xx IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.-
dc.subjectProteins-
dc.subjectForce-
dc.subjectSubstrates-
dc.subjectDrugs-
dc.subjectSpectroscopy-
dc.titleOptimization of Protein–Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy-
dc.typeArticle-
dc.identifier.emailSun, Z: sunzy@hku.hk-
dc.identifier.emailXi, N: xining@hku.hk-
dc.identifier.authorityXi, N=rp02044-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1109/TNANO.2019.2915507-
dc.identifier.scopuseid_2-s2.0-85066821319-
dc.identifier.hkuros300618-
dc.identifier.volume18-
dc.identifier.spage509-
dc.identifier.epage517-
dc.publisher.placeUnited States-

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