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Article: An electro-osmotic microfluidic system to characterize cancer cell migration under confinement

TitleAn electro-osmotic microfluidic system to characterize cancer cell migration under confinement
Authors
KeywordsCell adhesion
Electroosmosis
Fluidic devices
Friction
Ion engines
Issue Date2019
PublisherThe Royal Society. The Journal's web site is located at http://publishing.royalsociety.org/index.cfm?page=1572
Citation
Journal of the Royal Society Interface, 2019, v. 16 n. 155, p. article no. 20190062 How to Cite?
AbstractWe have developed a novel electro-osmotic microfluidic system to apply precisely controlled osmolarity gradients to cancer cells in micro-channels. We observed that albeit adhesion is not required for cells to migrate in such a confined microenvironment, the migrating velocity of cells is strongly influenced by the interactions between the cells and the channel wall, with a stronger adhesion leading to diminished cell motility. Furthermore, through examining more than 20 different types of cancer cells, we found a linear positive correlation between the protein concentration of the aquaporin-4 (AQP4) and the cell migrating speed. Knockdown of AQP4 in invasive re-populated cancer stem cells reduced their migration capability down to the level that is comparable to their parental cancer cells. Interestingly, these observations can all be quantitatively explained by the osmotic engine model where the cell movement is assumed to be driven by cross-membrane ion/water transport, while adhesion acts as a frictional resistance against the cell motility. By providing versatile and controllable features in regulating and characterizing the migration capability of cells, our system may serve as a useful tool in quantifying how cell motility is influenced by different physical and biochemical factors, as well as elucidating the mechanisms behind, in the future.
Persistent Identifierhttp://hdl.handle.net/10722/272221
ISSN
2017 Impact Factor: 3.355
2015 SCImago Journal Rankings: 1.622
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorHui, TH-
dc.contributor.authorCho, WC-
dc.contributor.authorFong, HW-
dc.contributor.authorYu, M-
dc.contributor.authorKwan, KW-
dc.contributor.authorNgan, KC-
dc.contributor.authorWong, KH-
dc.contributor.authorTan, Y-
dc.contributor.authorYao, S-
dc.contributor.authorJiang, H-
dc.contributor.authorGu, Z-
dc.contributor.authorLin, Y-
dc.date.accessioned2019-07-20T10:38:03Z-
dc.date.available2019-07-20T10:38:03Z-
dc.date.issued2019-
dc.identifier.citationJournal of the Royal Society Interface, 2019, v. 16 n. 155, p. article no. 20190062-
dc.identifier.issn1742-5689-
dc.identifier.urihttp://hdl.handle.net/10722/272221-
dc.description.abstractWe have developed a novel electro-osmotic microfluidic system to apply precisely controlled osmolarity gradients to cancer cells in micro-channels. We observed that albeit adhesion is not required for cells to migrate in such a confined microenvironment, the migrating velocity of cells is strongly influenced by the interactions between the cells and the channel wall, with a stronger adhesion leading to diminished cell motility. Furthermore, through examining more than 20 different types of cancer cells, we found a linear positive correlation between the protein concentration of the aquaporin-4 (AQP4) and the cell migrating speed. Knockdown of AQP4 in invasive re-populated cancer stem cells reduced their migration capability down to the level that is comparable to their parental cancer cells. Interestingly, these observations can all be quantitatively explained by the osmotic engine model where the cell movement is assumed to be driven by cross-membrane ion/water transport, while adhesion acts as a frictional resistance against the cell motility. By providing versatile and controllable features in regulating and characterizing the migration capability of cells, our system may serve as a useful tool in quantifying how cell motility is influenced by different physical and biochemical factors, as well as elucidating the mechanisms behind, in the future.-
dc.languageeng-
dc.publisherThe Royal Society. The Journal's web site is located at http://publishing.royalsociety.org/index.cfm?page=1572-
dc.relation.ispartofJournal of the Royal Society Interface-
dc.subjectCell adhesion-
dc.subjectElectroosmosis-
dc.subjectFluidic devices-
dc.subjectFriction-
dc.subjectIon engines-
dc.titleAn electro-osmotic microfluidic system to characterize cancer cell migration under confinement-
dc.typeArticle-
dc.identifier.emailHui, TH: bluesp12@HKUCC-COM.hku.hk-
dc.identifier.emailKwan, KW: kwan15@hku.hk-
dc.identifier.emailLin, Y: ylin@hkucc.hku.hk-
dc.identifier.authorityLin, Y=rp00080-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1098/rsif.2019.0062-
dc.identifier.pmid31164075-
dc.identifier.pmcidPMC6597772-
dc.identifier.scopuseid_2-s2.0-85067521790-
dc.identifier.hkuros298700-
dc.identifier.volume16-
dc.identifier.issue155-
dc.identifier.spagearticle no. 20190062-
dc.identifier.epagearticle no. 20190062-
dc.publisher.placeUnited Kingdom-

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