File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Transmission of a Novel Genotype of Hepatitis E Virus from Bactrian Camels to Cynomolgus Macaques

TitleTransmission of a Novel Genotype of Hepatitis E Virus from Bactrian Camels to Cynomolgus Macaques
Authors
KeywordsBactrian camel
chronic infection
cross-species transmission
hepatitis E virus
zoonotic infections
Issue Date2019
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal of Virology, 2019, v. 93 n. 7, p. article no. e02014-18 How to Cite?
AbstractHepatitis E virus (HEV) is zoonotic and a major cause of acute viral hepatitis worldwide. Recently, we identified a novel HEV genotype 8 (HEV8) in Bactrian camels in Xinjiang, China. However, the epidemiology, pathogenicity, and zoonotic potential of HEV8 are unclear. Here, we present the prevalence of HEV8 in China and investigate its pathogenicity and cross-species transmission in cynomolgus macaques. Fresh fecal and milk samples from Bactrian camels collected from four provinces/regions in China were screened for HEV RNA by reverse transcriptase PCR (RT-PCR). An HEV8-positive sample was used to inoculate two cynomolgus macaques to examine the potential for cross-species infection. The pathogenicity of HEV8 was analyzed by testing HEV markers and liver function during the study period and histopathology of liver biopsy specimens at 3, 13, and 25 weeks postinoculation. Extrahepatic replication was tested by using reverse transcriptase quantitative PCR (RT-qPCR) and immunofluorescence assays. The overall prevalence of HEV8 RNA in Chinese Bactrian camels was 1.4% (4/295), and positive samples were found in three different provinces/regions in China. Histopathology confirmed acute and chronic HEV8 infections in the two monkeys. Multiple tissues were positive for HEV RNA and ORF2 proteins. Renal pathology was observed in the monkey with chronic hepatitis. Whole-genome sequencing showed only 1 to 3 mutations in the HEV8 in the fecal samples from the two monkeys compared to that from the camel. HEV8 is circulating in multiple regions in China. Infection of two monkeys with HEV8 induced chronic and systemic infections, demonstrating the high potential zoonotic risk of HEV8.
Persistent Identifierhttp://hdl.handle.net/10722/271998
ISSN
2017 Impact Factor: 4.368
2015 SCImago Journal Rankings: 3.347
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorWang, L-
dc.contributor.authorTeng, JLL-
dc.contributor.authorLau, SKP-
dc.contributor.authorSridhar, S-
dc.contributor.authorFu, H-
dc.contributor.authorGong, W-
dc.contributor.authorLi, M-
dc.contributor.authorXu, Q-
dc.contributor.authorHe, Y-
dc.contributor.authorZhuang, H-
dc.contributor.authorWoo, PCY-
dc.contributor.authorWang, L-
dc.date.accessioned2019-07-20T10:33:41Z-
dc.date.available2019-07-20T10:33:41Z-
dc.date.issued2019-
dc.identifier.citationJournal of Virology, 2019, v. 93 n. 7, p. article no. e02014-18-
dc.identifier.issn0022-538X-
dc.identifier.urihttp://hdl.handle.net/10722/271998-
dc.description.abstractHepatitis E virus (HEV) is zoonotic and a major cause of acute viral hepatitis worldwide. Recently, we identified a novel HEV genotype 8 (HEV8) in Bactrian camels in Xinjiang, China. However, the epidemiology, pathogenicity, and zoonotic potential of HEV8 are unclear. Here, we present the prevalence of HEV8 in China and investigate its pathogenicity and cross-species transmission in cynomolgus macaques. Fresh fecal and milk samples from Bactrian camels collected from four provinces/regions in China were screened for HEV RNA by reverse transcriptase PCR (RT-PCR). An HEV8-positive sample was used to inoculate two cynomolgus macaques to examine the potential for cross-species infection. The pathogenicity of HEV8 was analyzed by testing HEV markers and liver function during the study period and histopathology of liver biopsy specimens at 3, 13, and 25 weeks postinoculation. Extrahepatic replication was tested by using reverse transcriptase quantitative PCR (RT-qPCR) and immunofluorescence assays. The overall prevalence of HEV8 RNA in Chinese Bactrian camels was 1.4% (4/295), and positive samples were found in three different provinces/regions in China. Histopathology confirmed acute and chronic HEV8 infections in the two monkeys. Multiple tissues were positive for HEV RNA and ORF2 proteins. Renal pathology was observed in the monkey with chronic hepatitis. Whole-genome sequencing showed only 1 to 3 mutations in the HEV8 in the fecal samples from the two monkeys compared to that from the camel. HEV8 is circulating in multiple regions in China. Infection of two monkeys with HEV8 induced chronic and systemic infections, demonstrating the high potential zoonotic risk of HEV8.-
dc.languageeng-
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/-
dc.relation.ispartofJournal of Virology-
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.-
dc.subjectBactrian camel-
dc.subjectchronic infection-
dc.subjectcross-species transmission-
dc.subjecthepatitis E virus-
dc.subjectzoonotic infections-
dc.titleTransmission of a Novel Genotype of Hepatitis E Virus from Bactrian Camels to Cynomolgus Macaques-
dc.typeArticle-
dc.identifier.emailTeng, JLL: llteng@hku.hk-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailSridhar, S: sid8998@hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityTeng, JLL=rp00277-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authoritySridhar, S=rp02249-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JVI.02014-18-
dc.identifier.pmid30700602-
dc.identifier.pmcidPMC6430554-
dc.identifier.scopuseid_2-s2.0-85063631215-
dc.identifier.hkuros298350-
dc.identifier.volume93-
dc.identifier.issue7-
dc.identifier.spagearticle no. e02014-
dc.identifier.epage18-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats