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Article: Cell Lineage-specific Genome-wide DNA Methylation Analysis of Patients with Paediatric-onset Systemic Lupus Erythematosus

TitleCell Lineage-specific Genome-wide DNA Methylation Analysis of Patients with Paediatric-onset Systemic Lupus Erythematosus
Authors
KeywordsBlood
Cell composition
Cell lineage-specific
DNA methylation
Lupus
MethylationEPIC
Paediatric-onset
SLE
Issue Date2019
PublisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandfonline.com/kepi
Citation
Epigenetics, 2019, v. 14 n. 4, p. 341-351 How to Cite?
AbstractPatients with paediatric-onset systemic lupus erythematosus (SLE) often present with more severe clinical courses than adult-onset patients. Although genome-wide DNA methylation (DNAm) profiling has been performed in adult-onset SLE patients, parallel data on paediatric-onset SLE are not available. Therefore, we undertook a genome-wide DNAm study in paediatric-onset SLE patients across multiple blood cell lineages. The DNAm profiles of four purified immune cell lineages (CD4 + T cells, CD8 + T cells, B cells and neutrophils) and whole blood were compared in 16 Chinese patients with paediatric-onset SLE and 13 healthy controls using the Illumina HumanMethylationEPIC BeadChip. Comparison of DNAm in whole blood and within each independent cell lineage identified a consistent pattern of loss of DNAm at 21 CpG sites overlapping 15 genes, which represented a robust, disease-specific DNAm signature for paediatric-onset SLE in our cohort. In addition, cell lineage-specific changes, involving both loss and gain of DNAm, were observed in both novel genes and genes with well-described roles in SLE pathogenesis. This study also highlights the importance of studying DNAm changes in different immune cell lineages rather than only whole blood, since cell type-specific DNAm changes facilitated the elucidation of the cell type-specific molecular pathophysiology of SLE.
Persistent Identifierhttp://hdl.handle.net/10722/271955
ISSN
2017 Impact Factor: 4.918
2015 SCImago Journal Rankings: 2.672
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYeung, KS-
dc.contributor.authorLee, TL-
dc.contributor.authorMok, MY-
dc.contributor.authorMak, CCY-
dc.contributor.authorYang, W-
dc.contributor.authorChong, PCY-
dc.contributor.authorLee, PPW-
dc.contributor.authorHo, MHK-
dc.contributor.authorChoufani, S-
dc.contributor.authorLau, CS-
dc.contributor.authorLau, YL-
dc.contributor.authorWeksberg, R-
dc.contributor.authorChung, BHY-
dc.date.accessioned2019-07-20T10:32:48Z-
dc.date.available2019-07-20T10:32:48Z-
dc.date.issued2019-
dc.identifier.citationEpigenetics, 2019, v. 14 n. 4, p. 341-351-
dc.identifier.issn1559-2294-
dc.identifier.urihttp://hdl.handle.net/10722/271955-
dc.description.abstractPatients with paediatric-onset systemic lupus erythematosus (SLE) often present with more severe clinical courses than adult-onset patients. Although genome-wide DNA methylation (DNAm) profiling has been performed in adult-onset SLE patients, parallel data on paediatric-onset SLE are not available. Therefore, we undertook a genome-wide DNAm study in paediatric-onset SLE patients across multiple blood cell lineages. The DNAm profiles of four purified immune cell lineages (CD4 + T cells, CD8 + T cells, B cells and neutrophils) and whole blood were compared in 16 Chinese patients with paediatric-onset SLE and 13 healthy controls using the Illumina HumanMethylationEPIC BeadChip. Comparison of DNAm in whole blood and within each independent cell lineage identified a consistent pattern of loss of DNAm at 21 CpG sites overlapping 15 genes, which represented a robust, disease-specific DNAm signature for paediatric-onset SLE in our cohort. In addition, cell lineage-specific changes, involving both loss and gain of DNAm, were observed in both novel genes and genes with well-described roles in SLE pathogenesis. This study also highlights the importance of studying DNAm changes in different immune cell lineages rather than only whole blood, since cell type-specific DNAm changes facilitated the elucidation of the cell type-specific molecular pathophysiology of SLE.-
dc.languageeng-
dc.publisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandfonline.com/kepi-
dc.relation.ispartofEpigenetics-
dc.subjectBlood-
dc.subjectCell composition-
dc.subjectCell lineage-specific-
dc.subjectDNA methylation-
dc.subjectLupus-
dc.subjectMethylationEPIC-
dc.subjectPaediatric-onset-
dc.subjectSLE-
dc.titleCell Lineage-specific Genome-wide DNA Methylation Analysis of Patients with Paediatric-onset Systemic Lupus Erythematosus-
dc.typeArticle-
dc.identifier.emailYeung, KS: ksyyeung@hku.hk-
dc.identifier.emailLee, TL: leetsz@hkucc.hku.hk-
dc.identifier.emailMok, MY: temy@hkucc.hku.hk-
dc.identifier.emailMak, CCY: cmakl@hku.hk-
dc.identifier.emailYang, W: yangwl@hku.hk-
dc.identifier.emailChong, PCY: chongpcy@hku.hk-
dc.identifier.emailLee, PPW: ppwlee@hku.hk-
dc.identifier.emailHo, MHK: marcoho@hku.hk-
dc.identifier.emailLau, CS: cslau@hku.hk-
dc.identifier.emailLau, YL: lauylung@hku.hk-
dc.identifier.emailChung, BHY: bhychung@hku.hk-
dc.identifier.authorityMok, MY=rp00490-
dc.identifier.authorityYang, W=rp00524-
dc.identifier.authorityLee, PPW=rp00462-
dc.identifier.authorityLau, CS=rp01348-
dc.identifier.authorityLau, YL=rp00361-
dc.identifier.authorityChung, BHY=rp00473-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/15592294.2019.1585176-
dc.identifier.pmid30806140-
dc.identifier.pmcidPMC6557544-
dc.identifier.scopuseid_2-s2.0-85063105328-
dc.identifier.hkuros298763-
dc.identifier.volume14-
dc.identifier.issue4-
dc.identifier.spage341-
dc.identifier.epage351-
dc.identifier.isiWOS:000468498100002-
dc.publisher.placeUnited States-

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