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Article: Green fluorescent probe for imaging His6-tagged proteins inside living cells

TitleGreen fluorescent probe for imaging His6-tagged proteins inside living cells
Authors
Issue Date2019
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/loi/ascefj
Citation
ACS Sensors, 2019, v. 4 n. 5, p. 1190-1196 How to Cite?
AbstractSmall molecule-based fluorescent probes offer great opportunities for specifically tracking proteins in living systems with minimal perturbation on the protein function and localization. Herein, we report a small green fluorescent probe (Ni2+-NTA-AF) consisting of a Ni2+-NTA moiety, a fluorescein, and an arylazide group, that binds specifically to His6-tagged proteins with fluorescence enhancement in vitro upon photoactivation of the arylazide group. Importantly, the probe can cross the cell membranes and stoichiometrically label His6-tagged proteins rapidly (∼15 min) in living prokaryotic and eukaryotic cells exemplified by a DNA repair protein Xeroderma pigmentosum group A (XPA). Using the probe, we successfully visualized Sirtuin 5, which is localized to the mitochondria. This probe exhibits high quantum yields and improved solubility, offering a new opportunity for imaging intracellular His6-tagged proteins inside living cells with better contrast.
Persistent Identifierhttp://hdl.handle.net/10722/270073
ISSN
2019 Impact Factor: 7.333

 

DC FieldValueLanguage
dc.contributor.authorYANG, Y-
dc.contributor.authorJiang, N-
dc.contributor.authorLai, YT-
dc.contributor.authorCHANG, YY-
dc.contributor.authorYang, X-
dc.contributor.authorSun, H-
dc.contributor.authorLi, H-
dc.date.accessioned2019-05-20T05:08:58Z-
dc.date.available2019-05-20T05:08:58Z-
dc.date.issued2019-
dc.identifier.citationACS Sensors, 2019, v. 4 n. 5, p. 1190-1196-
dc.identifier.issn2379-3694-
dc.identifier.urihttp://hdl.handle.net/10722/270073-
dc.description.abstractSmall molecule-based fluorescent probes offer great opportunities for specifically tracking proteins in living systems with minimal perturbation on the protein function and localization. Herein, we report a small green fluorescent probe (Ni2+-NTA-AF) consisting of a Ni2+-NTA moiety, a fluorescein, and an arylazide group, that binds specifically to His6-tagged proteins with fluorescence enhancement in vitro upon photoactivation of the arylazide group. Importantly, the probe can cross the cell membranes and stoichiometrically label His6-tagged proteins rapidly (∼15 min) in living prokaryotic and eukaryotic cells exemplified by a DNA repair protein Xeroderma pigmentosum group A (XPA). Using the probe, we successfully visualized Sirtuin 5, which is localized to the mitochondria. This probe exhibits high quantum yields and improved solubility, offering a new opportunity for imaging intracellular His6-tagged proteins inside living cells with better contrast.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/loi/ascefj-
dc.relation.ispartofACS Sensors-
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].-
dc.titleGreen fluorescent probe for imaging His6-tagged proteins inside living cells-
dc.typeArticle-
dc.identifier.emailJiang, N: leojiang@hku.hk-
dc.identifier.emailLai, YT: skytc13@hku.hk-
dc.identifier.emailSun, H: hsun@hku.hk-
dc.identifier.emailLi, H: hylichem@hku.hk-
dc.identifier.authoritySun, H=rp00777-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acssensors.8b01128-
dc.identifier.scopuseid_2-s2.0-85065852717-
dc.identifier.hkuros297870-
dc.identifier.volume4-
dc.identifier.issue5-
dc.identifier.spage1190-
dc.identifier.epage1196-
dc.publisher.placeUnited States-

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