File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces

TitleMetabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces
Authors
Issue Date2018
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/acbcct
Citation
ACS Chemical Biology, 2018, v. 13 n. 10, p. 3030-3037 How to Cite?
AbstractThe rise in antibiotic-resistant bacteria is causing worldwide concerns. The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets. Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans. They are involved in the host interaction, pathogenicity, antiphage defense mechanism, and immune escape mechanism. To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling. The effectiveness of this chemical reporter has been demonstrated in Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains. Expectedly, this strategy can provide a useful assay to detect phenotypic presence of Pse biosynthesis and screen for agents targeting this pathway.
Persistent Identifierhttp://hdl.handle.net/10722/269418
ISSN
2017 Impact Factor: 4.592
2015 SCImago Journal Rankings: 2.863
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorAndolina, G-
dc.contributor.authorWei, R-
dc.contributor.authorLiu, H-
dc.contributor.authorZhang, Q-
dc.contributor.authorYang, X-
dc.contributor.authorCao, H-
dc.contributor.authorChen, S-
dc.contributor.authorYan, A-
dc.contributor.authorLi, XD-
dc.contributor.authorLi, XC-
dc.date.accessioned2019-04-24T08:07:18Z-
dc.date.available2019-04-24T08:07:18Z-
dc.date.issued2018-
dc.identifier.citationACS Chemical Biology, 2018, v. 13 n. 10, p. 3030-3037-
dc.identifier.issn1554-8929-
dc.identifier.urihttp://hdl.handle.net/10722/269418-
dc.description.abstractThe rise in antibiotic-resistant bacteria is causing worldwide concerns. The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets. Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans. They are involved in the host interaction, pathogenicity, antiphage defense mechanism, and immune escape mechanism. To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling. The effectiveness of this chemical reporter has been demonstrated in Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains. Expectedly, this strategy can provide a useful assay to detect phenotypic presence of Pse biosynthesis and screen for agents targeting this pathway.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/acbcct-
dc.relation.ispartofACS Chemical Biology-
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].-
dc.titleMetabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces-
dc.typeArticle-
dc.identifier.emailWei, R: wrh2010@hku.hk-
dc.identifier.emailZhang, Q: qingz@hku.hk-
dc.identifier.emailCao, H: hcao@hku.hk-
dc.identifier.emailYan, A: ayan8@hku.hk-
dc.identifier.emailLi, XD: xiangli@hku.hk-
dc.identifier.emailLi, XC: xuechenl@hku.hk-
dc.identifier.authorityZhang, Q=rp02542-
dc.identifier.authorityYan, A=rp00823-
dc.identifier.authorityLi, XD=rp01562-
dc.identifier.authorityLi, XC=rp00742-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acschembio.8b00822-
dc.identifier.pmid30230814-
dc.identifier.scopuseid_2-s2.0-85054735129-
dc.identifier.hkuros297532-
dc.identifier.volume13-
dc.identifier.issue10-
dc.identifier.spage3030-
dc.identifier.epage3037-
dc.identifier.isiWOS:000448488600024-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats