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- Publisher Website: 10.1021/acschembio.8b00822
- Scopus: eid_2-s2.0-85054735129
- PMID: 30230814
- WOS: WOS:000448488600024
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Article: Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces
Title | Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces |
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Authors | |
Issue Date | 2018 |
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/acbcct |
Citation | ACS Chemical Biology, 2018, v. 13 n. 10, p. 3030-3037 How to Cite? |
Abstract | The rise in antibiotic-resistant bacteria is causing worldwide concerns. The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets. Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans. They are involved in the host interaction, pathogenicity, antiphage defense mechanism, and immune escape mechanism. To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling. The effectiveness of this chemical reporter has been demonstrated in Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains. Expectedly, this strategy can provide a useful assay to detect phenotypic presence of Pse biosynthesis and screen for agents targeting this pathway. |
Persistent Identifier | http://hdl.handle.net/10722/269418 |
ISSN | 2023 Impact Factor: 3.5 2023 SCImago Journal Rankings: 1.344 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Andolina, G | - |
dc.contributor.author | Wei, R | - |
dc.contributor.author | Liu, H | - |
dc.contributor.author | Zhang, Q | - |
dc.contributor.author | Yang, X | - |
dc.contributor.author | Cao, H | - |
dc.contributor.author | Chen, S | - |
dc.contributor.author | Yan, A | - |
dc.contributor.author | Li, XD | - |
dc.contributor.author | Li, XC | - |
dc.date.accessioned | 2019-04-24T08:07:18Z | - |
dc.date.available | 2019-04-24T08:07:18Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | ACS Chemical Biology, 2018, v. 13 n. 10, p. 3030-3037 | - |
dc.identifier.issn | 1554-8929 | - |
dc.identifier.uri | http://hdl.handle.net/10722/269418 | - |
dc.description.abstract | The rise in antibiotic-resistant bacteria is causing worldwide concerns. The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets. Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans. They are involved in the host interaction, pathogenicity, antiphage defense mechanism, and immune escape mechanism. To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling. The effectiveness of this chemical reporter has been demonstrated in Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains. Expectedly, this strategy can provide a useful assay to detect phenotypic presence of Pse biosynthesis and screen for agents targeting this pathway. | - |
dc.language | eng | - |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/acbcct | - |
dc.relation.ispartof | ACS Chemical Biology | - |
dc.title | Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces | - |
dc.type | Article | - |
dc.identifier.email | Wei, R: wrh2010@hku.hk | - |
dc.identifier.email | Zhang, Q: qingz@hku.hk | - |
dc.identifier.email | Cao, H: hcao@hku.hk | - |
dc.identifier.email | Yan, A: ayan8@hku.hk | - |
dc.identifier.email | Li, XD: xiangli@hku.hk | - |
dc.identifier.email | Li, XC: xuechenl@hku.hk | - |
dc.identifier.authority | Zhang, Q=rp02542 | - |
dc.identifier.authority | Yan, A=rp00823 | - |
dc.identifier.authority | Li, XD=rp01562 | - |
dc.identifier.authority | Li, XC=rp00742 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1021/acschembio.8b00822 | - |
dc.identifier.pmid | 30230814 | - |
dc.identifier.scopus | eid_2-s2.0-85054735129 | - |
dc.identifier.hkuros | 297532 | - |
dc.identifier.volume | 13 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 3030 | - |
dc.identifier.epage | 3037 | - |
dc.identifier.isi | WOS:000448488600024 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1554-8929 | - |