File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Qualitative and quantitative analysis of chemical constituents of Centipeda minima by HPLC-QTOF-MS & HPLC-DAD

TitleQualitative and quantitative analysis of chemical constituents of Centipeda minima by HPLC-QTOF-MS & HPLC-DAD
Authors
KeywordsCentipeda minima
Quadrupole time-of-flight mass spectrometry (QTOF MS)
Quality control
Herbal analysis
Isochlorogenic acid A
Brevilin A
Issue Date2016
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpba
Citation
Journal of Pharmaceutical and Biomedical Analysis, 2016, v. 125, p. 400-407 How to Cite?
AbstractA high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) method in both positive and negative ion modes was established to investigate the major constituents in the ethanolic extract of Centipeda minima (EBSC). Twelve common components including flavones and their glycosides, phenolic and polyphenolic acids, and sesquiterpene lactone were identified in ten batches of samples based on comparison with the retention time and accurate mass of external standards (mass accuracy within 3 ppm) or the fragmentation patterns of tandem MS. Meanwhile, a simple, accurate and reliable HPLC-DAD method was also developed to determine the content of 10 chemical markers simultaneously. Results obtained from method validations including linearity, accuracy and precision showed that this new method is reliable and robust. Isochlorogenic acid A and brevilin A were found to be the most abundant in the ethanol extract of EBSC and could be served as markers for quality control of EBSC.
Persistent Identifierhttp://hdl.handle.net/10722/267529
ISSN
2017 Impact Factor: 2.831
2015 SCImago Journal Rankings: 1.049
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, C-
dc.contributor.authorJin, D-
dc.contributor.authorDong, N-
dc.contributor.authorChen, S-
dc.contributor.authorMok, DKW-
dc.date.accessioned2019-02-19T09:54:00Z-
dc.date.available2019-02-19T09:54:00Z-
dc.date.issued2016-
dc.identifier.citationJournal of Pharmaceutical and Biomedical Analysis, 2016, v. 125, p. 400-407-
dc.identifier.issn0731-7085-
dc.identifier.urihttp://hdl.handle.net/10722/267529-
dc.description.abstractA high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) method in both positive and negative ion modes was established to investigate the major constituents in the ethanolic extract of Centipeda minima (EBSC). Twelve common components including flavones and their glycosides, phenolic and polyphenolic acids, and sesquiterpene lactone were identified in ten batches of samples based on comparison with the retention time and accurate mass of external standards (mass accuracy within 3 ppm) or the fragmentation patterns of tandem MS. Meanwhile, a simple, accurate and reliable HPLC-DAD method was also developed to determine the content of 10 chemical markers simultaneously. Results obtained from method validations including linearity, accuracy and precision showed that this new method is reliable and robust. Isochlorogenic acid A and brevilin A were found to be the most abundant in the ethanol extract of EBSC and could be served as markers for quality control of EBSC.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpba-
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis-
dc.subjectCentipeda minima-
dc.subjectQuadrupole time-of-flight mass spectrometry (QTOF MS)-
dc.subjectQuality control-
dc.subjectHerbal analysis-
dc.subjectIsochlorogenic acid A-
dc.subjectBrevilin A-
dc.titleQualitative and quantitative analysis of chemical constituents of Centipeda minima by HPLC-QTOF-MS & HPLC-DAD-
dc.typeArticle-
dc.identifier.emailDong, N: npdong@hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jpba.2016.04.029-
dc.identifier.pmid27131150-
dc.identifier.scopuseid_2-s2.0-84964553614-
dc.identifier.hkuros296840-
dc.identifier.volume125-
dc.identifier.spage400-
dc.identifier.epage407-
dc.identifier.isiWOS:000376474400048-
dc.publisher.placeNetherlands-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats