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Article: Cyclic Adenosine Monophosphate Promotes Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via Suppression of Transforming Growth Factor Beta 1 Signaling
Title | Cyclic Adenosine Monophosphate Promotes Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via Suppression of Transforming Growth Factor Beta 1 Signaling |
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Authors | |
Keywords | Cyclic adenosine monophosphate differentiation stem cell tissue engineering |
Issue Date | 2019 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.jendodon.com |
Citation | Journal of Endodontics, 2019, v. 45 n. 2, p. 150-155 How to Cite? |
Abstract | Introduction: Stem cells from the apical papilla (SCAPs) possess strong odonto/osteogenic differentiation potential. This study investigated the effect of cyclic adenosine monophosphate (cAMP) on odonto/osteogenic differentiation of SCAPs and the underlining interplay between cAMP and transforming growth factor beta 1 (TGF-β1). Methods: SCAPs were stimulated with an activator of cAMP (forskolin) in the presence of either TGF-β1 or a TGF-β1 inhibitor. The amounts of calcium mineral deposition and alkaline phosphatase activity were determined. Quantitative real-time polymerase chain reaction was performed to elucidate cAMP on the TGF-β1-mediated odonto/osteogenic differentiation of SCAPs. The effect of cAMP on the phosphorylation of Smad2/Smad3 and extracellular-regulated kinase (ERK)/P38 induced by TGF-β1 was analyzed by Western blotting. Results: Cotreatment with forskolin and a TGF-β1 inhibitor enhanced alkaline phosphatase activity and deposition of calcium minerals in SCAPs. Moreover, the TGF-β1 inhibitor synergized the effect of forskolin on the expression of type I collagen and runt-related transcription factor 2. The results of Western blotting revealed that forskolin attenuated the unregulated expression of the phosphorylation of Smad3 and ERK induced by TGF-β1, and a cAMP inhibitor (H89) antagonized this effect. Conclusions: This study showed that cAMP signaling exerts its up-regulating effects on the odonto/osteogenic differentiation of SCAPs by interfering with TGF-β1 signaling via inhibiting Smad3 and ERK phosphorylation. |
Persistent Identifier | http://hdl.handle.net/10722/267447 |
ISSN | 2023 Impact Factor: 3.5 2023 SCImago Journal Rankings: 1.356 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhang, J | - |
dc.contributor.author | Zhang, C | - |
dc.contributor.author | Quan, LL | - |
dc.contributor.author | Chu, CH | - |
dc.date.accessioned | 2019-02-18T09:02:14Z | - |
dc.date.available | 2019-02-18T09:02:14Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Journal of Endodontics, 2019, v. 45 n. 2, p. 150-155 | - |
dc.identifier.issn | 0099-2399 | - |
dc.identifier.uri | http://hdl.handle.net/10722/267447 | - |
dc.description.abstract | Introduction: Stem cells from the apical papilla (SCAPs) possess strong odonto/osteogenic differentiation potential. This study investigated the effect of cyclic adenosine monophosphate (cAMP) on odonto/osteogenic differentiation of SCAPs and the underlining interplay between cAMP and transforming growth factor beta 1 (TGF-β1). Methods: SCAPs were stimulated with an activator of cAMP (forskolin) in the presence of either TGF-β1 or a TGF-β1 inhibitor. The amounts of calcium mineral deposition and alkaline phosphatase activity were determined. Quantitative real-time polymerase chain reaction was performed to elucidate cAMP on the TGF-β1-mediated odonto/osteogenic differentiation of SCAPs. The effect of cAMP on the phosphorylation of Smad2/Smad3 and extracellular-regulated kinase (ERK)/P38 induced by TGF-β1 was analyzed by Western blotting. Results: Cotreatment with forskolin and a TGF-β1 inhibitor enhanced alkaline phosphatase activity and deposition of calcium minerals in SCAPs. Moreover, the TGF-β1 inhibitor synergized the effect of forskolin on the expression of type I collagen and runt-related transcription factor 2. The results of Western blotting revealed that forskolin attenuated the unregulated expression of the phosphorylation of Smad3 and ERK induced by TGF-β1, and a cAMP inhibitor (H89) antagonized this effect. Conclusions: This study showed that cAMP signaling exerts its up-regulating effects on the odonto/osteogenic differentiation of SCAPs by interfering with TGF-β1 signaling via inhibiting Smad3 and ERK phosphorylation. | - |
dc.language | eng | - |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.jendodon.com | - |
dc.relation.ispartof | Journal of Endodontics | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Cyclic adenosine monophosphate | - |
dc.subject | differentiation | - |
dc.subject | stem cell | - |
dc.subject | tissue engineering | - |
dc.title | Cyclic Adenosine Monophosphate Promotes Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via Suppression of Transforming Growth Factor Beta 1 Signaling | - |
dc.type | Article | - |
dc.identifier.email | Zhang, J: jing817@hku.hk | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.email | Chu, CH: chchu@hku.hk | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.identifier.authority | Chu, CH=rp00022 | - |
dc.description.nature | postprint | - |
dc.identifier.doi | 10.1016/j.joen.2018.10.008 | - |
dc.identifier.pmid | 30711170 | - |
dc.identifier.scopus | eid_2-s2.0-85060684029 | - |
dc.identifier.hkuros | 296957 | - |
dc.identifier.volume | 45 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 150 | - |
dc.identifier.epage | 155 | - |
dc.identifier.isi | WOS:000472683800011 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0099-2399 | - |