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Article: Database establishment for the secondary fungal DNA barcode translational elongation factor 1α (TEF1α)

TitleDatabase establishment for the secondary fungal DNA barcode translational elongation factor 1α (TEF1α)
Authors
KeywordsFungal DNA barcoding
Secondary fungal DNA barcode database
Translational elongation factor 1α
Issue Date2019
PublisherNRC Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?gen
Citation
Genome, 2019, v. 62 n. 3, p. 160-169 How to Cite?
AbstractWith new or emerging fungal infections, human/animal fungal pathogens are a growing threat worldwide. Current diagnostic tools are slow, non-specific at the species and subspecies levels, and require specific morphological expertise to accurately identify pathogens from pure cultures. DNA barcodes are easily amplified, universal, short species-specific DNA sequences, which enable rapid identification by comparison with a well-curated reference sequence collection. The primary fungal DNA barcode, ITS region, was introduced in 2012 and is now routinely used in diagnostic laboratories. However, the ITS region only accurately identifies around 75% of all medically relevant fungal species, which has prompted the development of a secondary barcode to increase the resolution power and suitability of DNA barcoding for fungal disease diagnostics. The translational elongation factor 1α (TEF1α) was selected in 2015 as a secondary fungal DNA barcode, but has not been implemented into practice, due to the absence of a reference database. Here, we have established a quality-controlled reference database for the secondary barcode which together with the ISHAM-ITS database, forms the “ISHAM BARCODE DATABASE”, available online at: http://its.mycologylab.org/. We encourage the mycology community for active contributions. The application of a dual DNA barcoding system enables accurate identification of all clinically important fungal pathogens.
Persistent Identifierhttp://hdl.handle.net/10722/266019
ISSN
2017 Impact Factor: 1.892
2015 SCImago Journal Rankings: 0.666
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMeyer, W-
dc.contributor.authorIrinyi, L-
dc.contributor.authorHoang, MTV-
dc.contributor.authorRobert, V-
dc.contributor.authorGarcia-Hermoso, D-
dc.contributor.authorDesnos-Ollivier, M-
dc.contributor.authorYurayart, C-
dc.contributor.authorTsang, CC-
dc.contributor.authorLee, CY-
dc.contributor.authorWoo, PCY-
dc.contributor.authorPchelin, IM-
dc.contributor.authorUhrlas, S-
dc.contributor.authorNenoff, P-
dc.contributor.authorChindamporn, A-
dc.contributor.authorChen, S-
dc.contributor.authorHebert, PDN-
dc.contributor.authorSorrell, TC-
dc.contributor.authorISHAM barcoding of pathogenic fungi working group-
dc.date.accessioned2018-12-17T02:16:33Z-
dc.date.available2018-12-17T02:16:33Z-
dc.date.issued2019-
dc.identifier.citationGenome, 2019, v. 62 n. 3, p. 160-169-
dc.identifier.issn0831-2796-
dc.identifier.urihttp://hdl.handle.net/10722/266019-
dc.description.abstractWith new or emerging fungal infections, human/animal fungal pathogens are a growing threat worldwide. Current diagnostic tools are slow, non-specific at the species and subspecies levels, and require specific morphological expertise to accurately identify pathogens from pure cultures. DNA barcodes are easily amplified, universal, short species-specific DNA sequences, which enable rapid identification by comparison with a well-curated reference sequence collection. The primary fungal DNA barcode, ITS region, was introduced in 2012 and is now routinely used in diagnostic laboratories. However, the ITS region only accurately identifies around 75% of all medically relevant fungal species, which has prompted the development of a secondary barcode to increase the resolution power and suitability of DNA barcoding for fungal disease diagnostics. The translational elongation factor 1α (TEF1α) was selected in 2015 as a secondary fungal DNA barcode, but has not been implemented into practice, due to the absence of a reference database. Here, we have established a quality-controlled reference database for the secondary barcode which together with the ISHAM-ITS database, forms the “ISHAM BARCODE DATABASE”, available online at: http://its.mycologylab.org/. We encourage the mycology community for active contributions. The application of a dual DNA barcoding system enables accurate identification of all clinically important fungal pathogens.-
dc.languageeng-
dc.publisherNRC Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?gen-
dc.relation.ispartofGenome-
dc.rightsGenome. Copyright © NRC Research Press.-
dc.subjectFungal DNA barcoding-
dc.subjectSecondary fungal DNA barcode database-
dc.subjectTranslational elongation factor 1α-
dc.titleDatabase establishment for the secondary fungal DNA barcode translational elongation factor 1α (TEF1α)-
dc.typeArticle-
dc.identifier.emailTsang, CC: microbioct@connect.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityTsang, CC=rp02492-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1139/gen-2018-0083-
dc.identifier.pmid30465691-
dc.identifier.scopuseid_2-s2.0-85065030454-
dc.identifier.hkuros296280-
dc.identifier.volume62-
dc.identifier.issue3-
dc.identifier.spage160-
dc.identifier.epage169-
dc.identifier.isiWOS:000465219400008-
dc.publisher.placeCanada-

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