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Article: MEK1/2 regulates microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte meiotic maturation

TitleMEK1/2 regulates microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte meiotic maturation
Authors
KeywordsMeiotic maturation
MEK
Spindle
Centrosomal proteins
Mouse oocytes
Issue Date2007
Citation
Cell Cycle, 2007, v. 6, n. 3, p. 330-338 How to Cite?
AbstractIt is well known that MAPK plays pivotal roles in oocyte maturation, but the function of MEK (MAPK kinase) remains unknown. We have studied the expression, subcellular localization and functional roles of MEK during meiotic maturation of mouse oocytes. Firstly, we found that MEK1/2 phoshorylation (p-MEK1/2, indicative of MEK activation) was low in GV (germinal vesicle) stage, increased 2h after GVBD (germinal vesicle breakdown), and reached the maximum at metaphase II. Secondly, we found that P-MEK1/2 was restricted in the GV prior to GVBD. In prometaphase I and metaphase I, P-MEK1/2 was mainly associated with the spindle, especially with the spindle poles. At anaphase I and telophase I, p-MEK1/2 became diffusely distributed in the region between the separating chromosomes, and then became associated with the midbody. The association of p-MEK1/2 with spindle poles was further confirmed by its colocalization with the centrosomal proteins, γ-tubulin and NuMA. Thirdly, we have investigated the possible functional role of MEK1/2 activation by intravenous administration and intrabursal injection of a specific MEK inhibitor, U0126, and by microinjection of MEK siRNA into oocytes. All these manipulations cause disorganized spindle poles and spindle structure, misaligned chromosomes and larger than normal polar bodies. Our results suggest that MEK1/2 may function as a centrosomal protein and may have roles in microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte maturation. ©2007 Landes Bioscience.
Persistent Identifierhttp://hdl.handle.net/10722/265787
ISSN
2017 Impact Factor: 3.304
2015 SCImago Journal Rankings: 2.244
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYu, Ling Zhu-
dc.contributor.authorXiong, Bo-
dc.contributor.authorGao, Wen Xue-
dc.contributor.authorWang, Chun Min-
dc.contributor.authorZhong, Zhi Sheng-
dc.contributor.authorHuo, Li Jun-
dc.contributor.authorWang, Qiang-
dc.contributor.authorHou, Yi-
dc.contributor.authorLiu, Kui-
dc.contributor.authorLiu, X. Johné-
dc.contributor.authorSchatten, Heide-
dc.contributor.authorChen, Da Yuan-
dc.contributor.authorSun, Qing Yuan-
dc.date.accessioned2018-12-03T01:21:41Z-
dc.date.available2018-12-03T01:21:41Z-
dc.date.issued2007-
dc.identifier.citationCell Cycle, 2007, v. 6, n. 3, p. 330-338-
dc.identifier.issn1538-4101-
dc.identifier.urihttp://hdl.handle.net/10722/265787-
dc.description.abstractIt is well known that MAPK plays pivotal roles in oocyte maturation, but the function of MEK (MAPK kinase) remains unknown. We have studied the expression, subcellular localization and functional roles of MEK during meiotic maturation of mouse oocytes. Firstly, we found that MEK1/2 phoshorylation (p-MEK1/2, indicative of MEK activation) was low in GV (germinal vesicle) stage, increased 2h after GVBD (germinal vesicle breakdown), and reached the maximum at metaphase II. Secondly, we found that P-MEK1/2 was restricted in the GV prior to GVBD. In prometaphase I and metaphase I, P-MEK1/2 was mainly associated with the spindle, especially with the spindle poles. At anaphase I and telophase I, p-MEK1/2 became diffusely distributed in the region between the separating chromosomes, and then became associated with the midbody. The association of p-MEK1/2 with spindle poles was further confirmed by its colocalization with the centrosomal proteins, γ-tubulin and NuMA. Thirdly, we have investigated the possible functional role of MEK1/2 activation by intravenous administration and intrabursal injection of a specific MEK inhibitor, U0126, and by microinjection of MEK siRNA into oocytes. All these manipulations cause disorganized spindle poles and spindle structure, misaligned chromosomes and larger than normal polar bodies. Our results suggest that MEK1/2 may function as a centrosomal protein and may have roles in microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte maturation. ©2007 Landes Bioscience.-
dc.languageeng-
dc.relation.ispartofCell Cycle-
dc.subjectMeiotic maturation-
dc.subjectMEK-
dc.subjectSpindle-
dc.subjectCentrosomal proteins-
dc.subjectMouse oocytes-
dc.titleMEK1/2 regulates microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte meiotic maturation-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.4161/cc.6.3.3805-
dc.identifier.pmid17297311-
dc.identifier.scopuseid_2-s2.0-33847040000-
dc.identifier.volume6-
dc.identifier.issue3-
dc.identifier.spage330-
dc.identifier.epage338-
dc.identifier.eissn1551-4005-
dc.identifier.isiWOS:000245495300016-

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