File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Molecular basis for the quantitative differential expression of different HLA-A and -B antigens in cells

TitleMolecular basis for the quantitative differential expression of different HLA-A and -B antigens in cells
Authors
Issue Date1998
Citation
FASEB Journal, 1998, v. 12, n. 5 How to Cite?
AbstractEarlier studies have shown that different specific HLA-A and -B antigens are differentially expressed in cells. Their relative quantities are genetically predetermined and inherited according to Mendelian law (Human Immunol. 38:243, 1993). To investigate the molecular basis for the quantitative differential expression of HLA antigens, we studied the turnover of different HLA-A and -B antigens expressed in HLA-phenotyped EBV-transformed lymphoblastoid cell lines (LCLs) and found that different HLA-A and -B antigens in LCLs have the same stability. When the relative quantities of different HLA-A and -B proteins were correlated with the relative amounts of their mRNAs in ten LCLs, they were proportionally correlated, except for cell lines positive for HLA-A24 and-B7. Measurement of the relative quantities of HLA-A and -B mRNAs in seven LCLs before and after DRB treatment showed that different specific HLA-A and -B mRNAs in five LCLs have the same turnover rates. The HLA-A and -B mRNA stability in the other two LCLs is slightly different. These findings suggest that the quantitative differential expression of HLA-A and -B antigens is primarily determined by the rate of HLA gene transcription. Stability or translation rate of mRNA also may play a role in regulating the differential expression of certain phenotypes of HLA antigens. Nuclear run-on experiments are being conducted to further confirm these findings.
Persistent Identifierhttp://hdl.handle.net/10722/265775
ISSN
2017 Impact Factor: 5.595
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorLiu, K.-
dc.contributor.authorKao, K. J.-
dc.date.accessioned2018-12-03T01:21:39Z-
dc.date.available2018-12-03T01:21:39Z-
dc.date.issued1998-
dc.identifier.citationFASEB Journal, 1998, v. 12, n. 5-
dc.identifier.issn0892-6638-
dc.identifier.urihttp://hdl.handle.net/10722/265775-
dc.description.abstractEarlier studies have shown that different specific HLA-A and -B antigens are differentially expressed in cells. Their relative quantities are genetically predetermined and inherited according to Mendelian law (Human Immunol. 38:243, 1993). To investigate the molecular basis for the quantitative differential expression of HLA antigens, we studied the turnover of different HLA-A and -B antigens expressed in HLA-phenotyped EBV-transformed lymphoblastoid cell lines (LCLs) and found that different HLA-A and -B antigens in LCLs have the same stability. When the relative quantities of different HLA-A and -B proteins were correlated with the relative amounts of their mRNAs in ten LCLs, they were proportionally correlated, except for cell lines positive for HLA-A24 and-B7. Measurement of the relative quantities of HLA-A and -B mRNAs in seven LCLs before and after DRB treatment showed that different specific HLA-A and -B mRNAs in five LCLs have the same turnover rates. The HLA-A and -B mRNA stability in the other two LCLs is slightly different. These findings suggest that the quantitative differential expression of HLA-A and -B antigens is primarily determined by the rate of HLA gene transcription. Stability or translation rate of mRNA also may play a role in regulating the differential expression of certain phenotypes of HLA antigens. Nuclear run-on experiments are being conducted to further confirm these findings.-
dc.languageeng-
dc.relation.ispartofFASEB Journal-
dc.titleMolecular basis for the quantitative differential expression of different HLA-A and -B antigens in cells-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-33749328469-
dc.identifier.volume12-
dc.identifier.issue5-
dc.identifier.spagenull-
dc.identifier.epagenull-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats