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Article: Molecular basis for the quantitative differential expression of different HLA-A and -B antigens in cells
Title | Molecular basis for the quantitative differential expression of different HLA-A and -B antigens in cells |
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Authors | |
Issue Date | 1998 |
Citation | FASEB Journal, 1998, v. 12, n. 5 How to Cite? |
Abstract | Earlier studies have shown that different specific HLA-A and -B antigens are differentially expressed in cells. Their relative quantities are genetically predetermined and inherited according to Mendelian law (Human Immunol. 38:243, 1993). To investigate the molecular basis for the quantitative differential expression of HLA antigens, we studied the turnover of different HLA-A and -B antigens expressed in HLA-phenotyped EBV-transformed lymphoblastoid cell lines (LCLs) and found that different HLA-A and -B antigens in LCLs have the same stability. When the relative quantities of different HLA-A and -B proteins were correlated with the relative amounts of their mRNAs in ten LCLs, they were proportionally correlated, except for cell lines positive for HLA-A24 and-B7. Measurement of the relative quantities of HLA-A and -B mRNAs in seven LCLs before and after DRB treatment showed that different specific HLA-A and -B mRNAs in five LCLs have the same turnover rates. The HLA-A and -B mRNA stability in the other two LCLs is slightly different. These findings suggest that the quantitative differential expression of HLA-A and -B antigens is primarily determined by the rate of HLA gene transcription. Stability or translation rate of mRNA also may play a role in regulating the differential expression of certain phenotypes of HLA antigens. Nuclear run-on experiments are being conducted to further confirm these findings. |
Persistent Identifier | http://hdl.handle.net/10722/265775 |
ISSN | 2023 Impact Factor: 4.4 2023 SCImago Journal Rankings: 1.412 |
DC Field | Value | Language |
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dc.contributor.author | Liu, K. | - |
dc.contributor.author | Kao, K. J. | - |
dc.date.accessioned | 2018-12-03T01:21:39Z | - |
dc.date.available | 2018-12-03T01:21:39Z | - |
dc.date.issued | 1998 | - |
dc.identifier.citation | FASEB Journal, 1998, v. 12, n. 5 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/10722/265775 | - |
dc.description.abstract | Earlier studies have shown that different specific HLA-A and -B antigens are differentially expressed in cells. Their relative quantities are genetically predetermined and inherited according to Mendelian law (Human Immunol. 38:243, 1993). To investigate the molecular basis for the quantitative differential expression of HLA antigens, we studied the turnover of different HLA-A and -B antigens expressed in HLA-phenotyped EBV-transformed lymphoblastoid cell lines (LCLs) and found that different HLA-A and -B antigens in LCLs have the same stability. When the relative quantities of different HLA-A and -B proteins were correlated with the relative amounts of their mRNAs in ten LCLs, they were proportionally correlated, except for cell lines positive for HLA-A24 and-B7. Measurement of the relative quantities of HLA-A and -B mRNAs in seven LCLs before and after DRB treatment showed that different specific HLA-A and -B mRNAs in five LCLs have the same turnover rates. The HLA-A and -B mRNA stability in the other two LCLs is slightly different. These findings suggest that the quantitative differential expression of HLA-A and -B antigens is primarily determined by the rate of HLA gene transcription. Stability or translation rate of mRNA also may play a role in regulating the differential expression of certain phenotypes of HLA antigens. Nuclear run-on experiments are being conducted to further confirm these findings. | - |
dc.language | eng | - |
dc.relation.ispartof | FASEB Journal | - |
dc.title | Molecular basis for the quantitative differential expression of different HLA-A and -B antigens in cells | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.scopus | eid_2-s2.0-33749328469 | - |
dc.identifier.volume | 12 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | null | - |
dc.identifier.epage | null | - |
dc.identifier.issnl | 0892-6638 | - |