File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Ignatzschineria cameli sp. nov., isolated from necrotic foot tissue of dromedaries (Camelus dromedarius) and associated maggots (Wohlfahrtia species) in Dubai

TitleIgnatzschineria cameli sp. nov., isolated from necrotic foot tissue of dromedaries (Camelus dromedarius) and associated maggots (Wohlfahrtia species) in Dubai
Authors
KeywordsCameli
Dromedaries
Ignatzschineria
Maggots
Novel
Issue Date2018
PublisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org
Citation
International Journal of Systematic and Evolutionary Microbiology, 2018, v. 68 n. 11, p. 3627-3634 How to Cite?
AbstractFive bacterial strains, UAE-HKU57T, UAE-HKU58, UAE-HKU59, UAE-HKU60 and UAE-HKU61, were isolated in Dubai, UAE, from necrotic foot tissue samples of four dromedaries (Camelus dromedarius) and associated maggots (Wohrlfartia species). They were non-sporulating, Gram-negative, non-motile bacilli. They grew well under aerobic conditions at 37 °C, but not anaerobically. The pH range for growth was pH 7.0-9.0 (optimum, pH 7.5-8.0) and the strains could tolerate NaCl concentrations (w/v) up to 2 % (optimum, 0.5 %). They were catalase- and cytochrome oxidase-positive, but caseinase-, gelatinase- and urease-negative. Their phenotypic characters were distinguishable from other closely related species. Phylogenetic analyses of the almost-complete 16S rRNA gene and partial 23S rRNA gene, gyrB, groEL and recA sequences revealed that the five isolates were most closely related to undescribed Ignatzschineria strain F8392 and Ignatzschineria indica, but in most phylogenies clustered separately from these close relatives. Average nucleotide identity analysis showed that genomes of the five isolates (2.47-2.52 Mb, G+C content 41.71-41.86 mol%) were 98.00-99.97% similar to each other, but ≤87.18 % similar to other Ignatzschineriaspecies/strains. Low DNA relatedness between the five isolates to other Ignatzschineriaspecies/strains was also supported by Genome-to-Genome Distance Calculator analysis. The chemotaxonomic traits of the five strains were highly similar. They were non-susceptible (intermediate or resistant) to tetracycline and resistant to trimethoprim/sulphamethoxazole. The name Ignatzschineria cameli sp. nov. is proposed to accommodate these five strains, with strain UAE-HKU57T (=CCOS1165T=NBRC 113042T) as the type strain.
Persistent Identifierhttp://hdl.handle.net/10722/264238
ISSN
2017 Impact Factor: 1.932
2015 SCImago Journal Rankings: 1.088
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsang, CC-
dc.contributor.authorTang, YM-
dc.contributor.authorFong, YH-
dc.contributor.authorKinne, J-
dc.contributor.authorLee, HH-
dc.contributor.authorJoseph, M-
dc.contributor.authorJose, S-
dc.contributor.authorSchuster, RK-
dc.contributor.authorTang, Y-
dc.contributor.authorSivakumar, S-
dc.contributor.authorChen, HKJ-
dc.contributor.authorTeng, LL-
dc.contributor.authorLau, SKP-
dc.contributor.authorWernery, U-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2018-10-22T07:51:45Z-
dc.date.available2018-10-22T07:51:45Z-
dc.date.issued2018-
dc.identifier.citationInternational Journal of Systematic and Evolutionary Microbiology, 2018, v. 68 n. 11, p. 3627-3634-
dc.identifier.issn1466-5026-
dc.identifier.urihttp://hdl.handle.net/10722/264238-
dc.description.abstractFive bacterial strains, UAE-HKU57T, UAE-HKU58, UAE-HKU59, UAE-HKU60 and UAE-HKU61, were isolated in Dubai, UAE, from necrotic foot tissue samples of four dromedaries (Camelus dromedarius) and associated maggots (Wohrlfartia species). They were non-sporulating, Gram-negative, non-motile bacilli. They grew well under aerobic conditions at 37 °C, but not anaerobically. The pH range for growth was pH 7.0-9.0 (optimum, pH 7.5-8.0) and the strains could tolerate NaCl concentrations (w/v) up to 2 % (optimum, 0.5 %). They were catalase- and cytochrome oxidase-positive, but caseinase-, gelatinase- and urease-negative. Their phenotypic characters were distinguishable from other closely related species. Phylogenetic analyses of the almost-complete 16S rRNA gene and partial 23S rRNA gene, gyrB, groEL and recA sequences revealed that the five isolates were most closely related to undescribed Ignatzschineria strain F8392 and Ignatzschineria indica, but in most phylogenies clustered separately from these close relatives. Average nucleotide identity analysis showed that genomes of the five isolates (2.47-2.52 Mb, G+C content 41.71-41.86 mol%) were 98.00-99.97% similar to each other, but ≤87.18 % similar to other Ignatzschineriaspecies/strains. Low DNA relatedness between the five isolates to other Ignatzschineriaspecies/strains was also supported by Genome-to-Genome Distance Calculator analysis. The chemotaxonomic traits of the five strains were highly similar. They were non-susceptible (intermediate or resistant) to tetracycline and resistant to trimethoprim/sulphamethoxazole. The name Ignatzschineria cameli sp. nov. is proposed to accommodate these five strains, with strain UAE-HKU57T (=CCOS1165T=NBRC 113042T) as the type strain.-
dc.languageeng-
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org-
dc.relation.ispartofInternational Journal of Systematic and Evolutionary Microbiology-
dc.rightsInternational Journal of Systematic and Evolutionary Microbiology. Copyright © Society for General Microbiology.-
dc.rightsThis is an author manuscript that has been accepted for publication in [Journal Title], copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in [Journal Title]. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at [Journal URL], and is freely available without a subscription.-
dc.subjectCameli-
dc.subjectDromedaries-
dc.subjectIgnatzschineria-
dc.subjectMaggots-
dc.subjectNovel-
dc.titleIgnatzschineria cameli sp. nov., isolated from necrotic foot tissue of dromedaries (Camelus dromedarius) and associated maggots (Wohlfahrtia species) in Dubai-
dc.typeArticle-
dc.identifier.emailTsang, CC: microbioct@connect.hku.hk-
dc.identifier.emailTang, Y: ashleyt@HKUCC-COM.hku.hk-
dc.identifier.emailChen, HKJ: jonchk@hku.hk-
dc.identifier.emailTeng, LL: llteng@hku.hk-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityTsang, CC=rp02492-
dc.identifier.authorityTeng, LL=rp00277-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1099/ijsem.0.003046-
dc.identifier.scopuseid_2-s2.0-85055818149-
dc.identifier.hkuros295447-
dc.identifier.volume68-
dc.identifier.issue11-
dc.identifier.spage3627-
dc.identifier.epage3634-
dc.identifier.isiWOS:000448920600038-
dc.publisher.placeUnited Kingdom-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats