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Article: A combined experimental and theoretical investigation on cellular blebbing

TitleA combined experimental and theoretical investigation on cellular blebbing
Authors
Issue Date2017
PublisherNature Publishing Group: Open Access Journals - Option C. The Journal's web site is located at http://www.nature.com/srep/index.html
Citation
Scientific Reports, 2017, v. 7 n. 1, article no. 16666 How to Cite?
AbstractAlthough accumulating evidence has demonstrated the important role of membrane blebbing in various cellular processes, the fundamental question of how the initiation/evolution of blebs are influenced by physical factors like membrane-cortex interactions and intracellular pressure remains unclear. Here, we report a combined modeling and experimental study to address this outstanding issue. Specifically, boundary integral method was used to track the motion of membrane (in 3D) during blebbing while possible rupture of the bilayer-cortex adhesion has also been taken into account. We showed that, for a given differential pressure across the cell membrane, the size of the weakened cortex must be over a critical value for blebbing to occur and the steady-state volume of a bleb is proportional to its initial growth rate, all in good agreement with recent experiments. The predicted shape evolution of blebs also matches well with our observations. Finally, a blebbing map, summarizing the essential physics involved, was obtained which exhibits three distinct regimes: no bleb formation corresponding to a low intracellular pressure or a small weakened cortex region; bleb formed with a fixed width when the disrupted cortex zone is very large; and a growing bleb resulted from progressive membrane-cortex detachment under intermediate weakened cortex size.
Persistent Identifierhttp://hdl.handle.net/10722/263381
ISSN
2017 Impact Factor: 4.122
2015 SCImago Journal Rankings: 2.073
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFang, C-
dc.contributor.authorHui, TH-
dc.contributor.authorWei, X-
dc.contributor.authorShao, X-
dc.contributor.authorLin, Y-
dc.date.accessioned2018-10-22T07:38:00Z-
dc.date.available2018-10-22T07:38:00Z-
dc.date.issued2017-
dc.identifier.citationScientific Reports, 2017, v. 7 n. 1, article no. 16666-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10722/263381-
dc.description.abstractAlthough accumulating evidence has demonstrated the important role of membrane blebbing in various cellular processes, the fundamental question of how the initiation/evolution of blebs are influenced by physical factors like membrane-cortex interactions and intracellular pressure remains unclear. Here, we report a combined modeling and experimental study to address this outstanding issue. Specifically, boundary integral method was used to track the motion of membrane (in 3D) during blebbing while possible rupture of the bilayer-cortex adhesion has also been taken into account. We showed that, for a given differential pressure across the cell membrane, the size of the weakened cortex must be over a critical value for blebbing to occur and the steady-state volume of a bleb is proportional to its initial growth rate, all in good agreement with recent experiments. The predicted shape evolution of blebs also matches well with our observations. Finally, a blebbing map, summarizing the essential physics involved, was obtained which exhibits three distinct regimes: no bleb formation corresponding to a low intracellular pressure or a small weakened cortex region; bleb formed with a fixed width when the disrupted cortex zone is very large; and a growing bleb resulted from progressive membrane-cortex detachment under intermediate weakened cortex size.-
dc.languageeng-
dc.publisherNature Publishing Group: Open Access Journals - Option C. The Journal's web site is located at http://www.nature.com/srep/index.html-
dc.relation.ispartofScientific Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleA combined experimental and theoretical investigation on cellular blebbing-
dc.typeArticle-
dc.identifier.emailLin, Y: ylin@hkucc.hku.hk-
dc.identifier.authorityLin, Y=rp00080-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41598-017-16825-0-
dc.identifier.scopuseid_2-s2.0-85037038020-
dc.identifier.hkuros293958-
dc.identifier.volume7-
dc.identifier.issue1-
dc.identifier.spagearticle no. 16666-
dc.identifier.epagearticle no. 16666-
dc.identifier.isiWOS:000416891400068-
dc.publisher.placeUnited Kingdom-

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