File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.ydbio.2017.08.037
- Scopus: eid_2-s2.0-85039778233
- PMID: 29291980
- WOS: WOS:000423009700023
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Identification of satellite cells from anole lizard skeletal muscle and demonstration of expanded musculoskeletal potential
Title | Identification of satellite cells from anole lizard skeletal muscle and demonstration of expanded musculoskeletal potential |
---|---|
Authors | |
Issue Date | 2018 |
Citation | Developmental Biology, 2018, v. 433, n. 2, p. 344-356 How to Cite? |
Abstract | © 2017 Elsevier Inc. The lizards are evolutionarily the closest vertebrates to humans that demonstrate the ability to regenerate entire appendages containing cartilage, muscle, skin, and nervous tissue. We previously isolated PAX7-positive cells from muscle of the green anole lizard, Anolis carolinensis, that can differentiate into multinucleated myotubes and express the muscle structural protein, myosin heavy chain. Studying gene expression in these satellite/progenitor cell populations from A. carolinensis can provide insight into the mechanisms regulating tissue regeneration. We generated a transcriptome from proliferating lizard myoprogenitor cells and compared them to transcriptomes from the mouse and human tissues from the ENCODE project using XGSA, a statistical method for cross-species gene set analysis. These analyses determined that the lizard progenitor cell transcriptome was most similar to mammalian satellite cells. Further examination of specific GO categories of genes demonstrated that among genes with the highest level of expression in lizard satellite cells were an increased number of genetic regulators of chondrogenesis, as compared to mouse satellite cells. In micromass culture, lizard PAX7-positive cells formed Alcian blue and collagen 2a1 positive nodules, without the addition of exogenous morphogens, unlike their mouse counterparts. Subsequent quantitative RT-PCR confirmed up-regulation of expression of chondrogenic regulatory genes in lizard cells, including bmp2, sox9, runx2, and cartilage specific structural genes, aggrecan and collagen 2a1. Taken together, these data suggest that tail regeneration in lizards involves significant alterations in gene regulation with expanded musculoskeletal potency. |
Persistent Identifier | http://hdl.handle.net/10722/262776 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 1.147 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Palade, Joanna | - |
dc.contributor.author | Djordjevic, Djordje | - |
dc.contributor.author | Hutchins, Elizabeth D. | - |
dc.contributor.author | George, Rajani M. | - |
dc.contributor.author | Cornelius, John A. | - |
dc.contributor.author | Rawls, Alan | - |
dc.contributor.author | Ho, Joshua W.K. | - |
dc.contributor.author | Kusumi, Kenro | - |
dc.contributor.author | Wilson-Rawls, Jeanne | - |
dc.date.accessioned | 2018-10-08T02:47:00Z | - |
dc.date.available | 2018-10-08T02:47:00Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Developmental Biology, 2018, v. 433, n. 2, p. 344-356 | - |
dc.identifier.issn | 0012-1606 | - |
dc.identifier.uri | http://hdl.handle.net/10722/262776 | - |
dc.description.abstract | © 2017 Elsevier Inc. The lizards are evolutionarily the closest vertebrates to humans that demonstrate the ability to regenerate entire appendages containing cartilage, muscle, skin, and nervous tissue. We previously isolated PAX7-positive cells from muscle of the green anole lizard, Anolis carolinensis, that can differentiate into multinucleated myotubes and express the muscle structural protein, myosin heavy chain. Studying gene expression in these satellite/progenitor cell populations from A. carolinensis can provide insight into the mechanisms regulating tissue regeneration. We generated a transcriptome from proliferating lizard myoprogenitor cells and compared them to transcriptomes from the mouse and human tissues from the ENCODE project using XGSA, a statistical method for cross-species gene set analysis. These analyses determined that the lizard progenitor cell transcriptome was most similar to mammalian satellite cells. Further examination of specific GO categories of genes demonstrated that among genes with the highest level of expression in lizard satellite cells were an increased number of genetic regulators of chondrogenesis, as compared to mouse satellite cells. In micromass culture, lizard PAX7-positive cells formed Alcian blue and collagen 2a1 positive nodules, without the addition of exogenous morphogens, unlike their mouse counterparts. Subsequent quantitative RT-PCR confirmed up-regulation of expression of chondrogenic regulatory genes in lizard cells, including bmp2, sox9, runx2, and cartilage specific structural genes, aggrecan and collagen 2a1. Taken together, these data suggest that tail regeneration in lizards involves significant alterations in gene regulation with expanded musculoskeletal potency. | - |
dc.language | eng | - |
dc.relation.ispartof | Developmental Biology | - |
dc.title | Identification of satellite cells from anole lizard skeletal muscle and demonstration of expanded musculoskeletal potential | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1016/j.ydbio.2017.08.037 | - |
dc.identifier.pmid | 29291980 | - |
dc.identifier.scopus | eid_2-s2.0-85039778233 | - |
dc.identifier.volume | 433 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 344 | - |
dc.identifier.epage | 356 | - |
dc.identifier.eissn | 1095-564X | - |
dc.identifier.isi | WOS:000423009700023 | - |
dc.identifier.issnl | 0012-1606 | - |