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Article: Tracking iron-associated proteomes in pathogens by a fluorescence approach

TitleTracking iron-associated proteomes in pathogens by a fluorescence approach
Authors
Issue Date2018
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/Publishing/Journals/MT/About.asp
Citation
Metallomics, 2018, v. 10 n. 1, p. 77-82 How to Cite?
AbstractPorphyromonas gingivalis is a key oral anaerobic bacterium involved in human periodontitis, which may affect up to 15% adults worldwide. Using the membrane permeable fluorescent probe Fe-TRACER, we identified 17 iron-associated proteins in Porphyromonas gingivalis. We demonstrated the specific binding of the probe towards iron-associated proteins using transferrin as an example and provided an X-ray structure of the fluorescent probe-bound transferrin. Our study provides a basis for the understanding of iron homeostasis in pathogens, and our approach based on the integration of fluorescence imaging with proteomics and bioinformatics can be readily extended to mine other metalloproteomes in microbials.
Persistent Identifierhttp://hdl.handle.net/10722/261362
ISSN
2017 Impact Factor: 4.069
2015 SCImago Journal Rankings: 1.120

 

DC FieldValueLanguage
dc.contributor.authorJiang, N-
dc.contributor.authorCheng, T-
dc.contributor.authorWang, M-
dc.contributor.authorChan, GCF-
dc.contributor.authorJin, L-
dc.contributor.authorLi, H-
dc.contributor.authorSun, H-
dc.date.accessioned2018-09-14T08:56:54Z-
dc.date.available2018-09-14T08:56:54Z-
dc.date.issued2018-
dc.identifier.citationMetallomics, 2018, v. 10 n. 1, p. 77-82-
dc.identifier.issn1756-5901-
dc.identifier.urihttp://hdl.handle.net/10722/261362-
dc.description.abstractPorphyromonas gingivalis is a key oral anaerobic bacterium involved in human periodontitis, which may affect up to 15% adults worldwide. Using the membrane permeable fluorescent probe Fe-TRACER, we identified 17 iron-associated proteins in Porphyromonas gingivalis. We demonstrated the specific binding of the probe towards iron-associated proteins using transferrin as an example and provided an X-ray structure of the fluorescent probe-bound transferrin. Our study provides a basis for the understanding of iron homeostasis in pathogens, and our approach based on the integration of fluorescence imaging with proteomics and bioinformatics can be readily extended to mine other metalloproteomes in microbials.-
dc.languageeng-
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/Publishing/Journals/MT/About.asp-
dc.relation.ispartofMetallomics-
dc.titleTracking iron-associated proteomes in pathogens by a fluorescence approach-
dc.typeArticle-
dc.identifier.emailCheng, T: chengtfc@hku.hk-
dc.identifier.emailChan, GCF: gcfchan@hku.hk-
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.emailLi, H: hylichem@hku.hk-
dc.identifier.emailSun, H: hsun@hku.hk-
dc.identifier.authorityChan, GCF=rp00431-
dc.identifier.authorityJin, L=rp00028-
dc.identifier.authoritySun, H=rp00777-
dc.identifier.doi10.1039/C7MT00275K-
dc.identifier.pmid29323384-
dc.identifier.hkuros291796-
dc.identifier.hkuros292257-
dc.identifier.volume10-
dc.identifier.issue1-
dc.identifier.spage77-
dc.identifier.epage82-
dc.publisher.placeUnited Kingdom-

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