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Conference Paper: A metagenomics study of the elbow of psoriasis subjects and their healthy relatives

TitleA metagenomics study of the elbow of psoriasis subjects and their healthy relatives
Authors
Issue Date2018
PublisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00015555.asp
Citation
The 5th World Psoriasis & Psoriatic Arthritis Conference 2018, Stockholm, Sweden, 27-30 June 2018. In Acta Dermato-Venereologica, 2018, v. 98 n. S219, p. 5 How to Cite?
AbstractIntroduction: Psoriasis is a multifaceted immune-mediated skin disease and has been associated with other comorbidities. Previous studies in psoriasis reported the abundance of microbial taxa above the species rank (family Firmicutes and genus Staphylococcus), or the combined abundance of multiple taxa (Corynebacterium, Propionibacterium, Staphylococcus, and Streptococcus) were associated with psoriasis [1–3]. Characterizing the skin microbiome of psoriasis subjects and family controls can further our understanding of microbiota’s role in this disease. Objectives: This study aims to reveal the difference of skin microbiome between skin lesion area and normal area of the elbow as well as family controls. Methods: DNA samples was extracted from 21 elbow-skin. 14 of 21 were from 7 psoriasis subjects’ lesion side and normal side. The rest were from a healthy family member whom the subject lives with. Shotgun metagenomic sequencing was adapted to resolute the skin microbiota. Partially overlapping t-test was used to investigate differentially abundant taxa. PERMANOVA was used to test the difference of microbial community composition influenced by psoriasis and sampling families. Results: Bacteria phyla Proteobacteria, Actinobacteria, and Bacteroidetes were three most abundant microbial taxa in both lesion and normal skin. Bacteria phyla Cyanobacteria and Candidatus Parcubacteria, family Hymenobacteraceae were less abundant on lesion skin than normal skin (p<0.05). Sampling families had significantly different elbow-skin microbial composition for bacterial genus level profile (p=0.001). Conclusions: Several bacterial taxa different from those previously discovered are more abundant on normal skin than lesion skin in the elbow. References: [1] Unexplored diversity and strain-level structure of the skin microbiome associated with psoriasis. NPJ Biofilms Microbiomes. 3,14 (2017). [2] Statnikov, A. et al. Microbiomic signatures of psoriasis: feasibility and methodology comparison. Sci. Rep. 3, 2620 (2013). [3] Alekseyenko, A. V. et al. Community differentiation of the cutaneous microbiota in psoriasis. Microbiome. 1, 31 (2013).
Persistent Identifierhttp://hdl.handle.net/10722/261055
ISSN
2019 Impact Factor: 4.016
2015 SCImago Journal Rankings: 1.047

 

DC FieldValueLanguage
dc.contributor.authorWang, H-
dc.contributor.authorNi, MY-
dc.contributor.authorFung, WT-
dc.contributor.authorYan, K-
dc.contributor.authorYao, X-
dc.contributor.authorWang, Y-
dc.contributor.authorKriegel, M-
dc.contributor.authorChan, HH-
dc.contributor.authorPang, HMH-
dc.date.accessioned2018-09-14T08:51:43Z-
dc.date.available2018-09-14T08:51:43Z-
dc.date.issued2018-
dc.identifier.citationThe 5th World Psoriasis & Psoriatic Arthritis Conference 2018, Stockholm, Sweden, 27-30 June 2018. In Acta Dermato-Venereologica, 2018, v. 98 n. S219, p. 5-
dc.identifier.issn0001-5555-
dc.identifier.urihttp://hdl.handle.net/10722/261055-
dc.description.abstractIntroduction: Psoriasis is a multifaceted immune-mediated skin disease and has been associated with other comorbidities. Previous studies in psoriasis reported the abundance of microbial taxa above the species rank (family Firmicutes and genus Staphylococcus), or the combined abundance of multiple taxa (Corynebacterium, Propionibacterium, Staphylococcus, and Streptococcus) were associated with psoriasis [1–3]. Characterizing the skin microbiome of psoriasis subjects and family controls can further our understanding of microbiota’s role in this disease. Objectives: This study aims to reveal the difference of skin microbiome between skin lesion area and normal area of the elbow as well as family controls. Methods: DNA samples was extracted from 21 elbow-skin. 14 of 21 were from 7 psoriasis subjects’ lesion side and normal side. The rest were from a healthy family member whom the subject lives with. Shotgun metagenomic sequencing was adapted to resolute the skin microbiota. Partially overlapping t-test was used to investigate differentially abundant taxa. PERMANOVA was used to test the difference of microbial community composition influenced by psoriasis and sampling families. Results: Bacteria phyla Proteobacteria, Actinobacteria, and Bacteroidetes were three most abundant microbial taxa in both lesion and normal skin. Bacteria phyla Cyanobacteria and Candidatus Parcubacteria, family Hymenobacteraceae were less abundant on lesion skin than normal skin (p<0.05). Sampling families had significantly different elbow-skin microbial composition for bacterial genus level profile (p=0.001). Conclusions: Several bacterial taxa different from those previously discovered are more abundant on normal skin than lesion skin in the elbow. References: [1] Unexplored diversity and strain-level structure of the skin microbiome associated with psoriasis. NPJ Biofilms Microbiomes. 3,14 (2017). [2] Statnikov, A. et al. Microbiomic signatures of psoriasis: feasibility and methodology comparison. Sci. Rep. 3, 2620 (2013). [3] Alekseyenko, A. V. et al. Community differentiation of the cutaneous microbiota in psoriasis. Microbiome. 1, 31 (2013).-
dc.languageeng-
dc.publisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00015555.asp-
dc.relation.ispartofActa Dermato-Venereologica-
dc.rightsThis is an electronic version of an article published in [include the complete citation information for the final version of the article as published in the print edition of the journal]. [JOURNAL TITLE] is available online at: http://www.informaworld.com/smpp/ with the open URL of your article.-
dc.titleA metagenomics study of the elbow of psoriasis subjects and their healthy relatives-
dc.typeConference_Paper-
dc.identifier.emailNi, MY: nimy@hku.hk-
dc.identifier.emailPang, HMH: herbpang@hku.hk-
dc.identifier.authorityNi, MY=rp01639-
dc.identifier.authorityPang, HMH=rp01857-
dc.identifier.doi10.2340/00015555-2978-
dc.identifier.hkuros291819-
dc.identifier.volume98-
dc.identifier.issueS219-
dc.identifier.spage5-
dc.identifier.epage5-
dc.publisher.placeUnited Kingdom-

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