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Article: Actin cytoskeleton stiffness grades metastatic potential of ovarian carcinoma Hey A8 cells via nanoindentation mapping

TitleActin cytoskeleton stiffness grades metastatic potential of ovarian carcinoma Hey A8 cells via nanoindentation mapping
Authors
KeywordsAFM indentation
Elastic modulus
Finite-element modelling
Metastasis
Ovarian cells
Issue Date2017
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/jbiomech
Citation
Journal of Biomechanics, 2017, v. 60, p. 219-226 How to Cite?
AbstractRecent studies have indicated that the nanoindentation measured stiffness of carcinoma adherent cells is in general lower than normal cells, thus suggesting that cell stiffness may serve as a bio-marker for carcinoma. However, the proper establishment of such a conclusion would require biophysical understanding of the underlying mechanism of the cell stiffness. In this work, we compared the elastic moduli of the actin cytoskeletons of Hey A8 ovarian carcinoma cells with and without metastasis (HM and NM), as measured by 2D atomic force microscopy (AFM) with low-depth nanoindentation via a rate-jump method. The results indicate clearly that HM cells showed lower actin cytoskeleton stiffness atop of their nucleus position and higher actin cytoskeleton stiffness at their rims, compared to NM cells, suggesting that the local stiffness on the cytoskeleton can reflect actin filament distribution. Immunofluorescence staining and scanning electron microscopy (SEM) also indicated that the difference in stiffness in Hey A8 cells with different metastasis is associated with their F-actin rearrangement. Finite-element modelling (FEM) shows that a migrating cell would have its actin filaments bundled together to form stress fibers, which would exhibit lower indentation stiffness than the less aligned arrangement of filaments in a non-migrating cell. The results here indicate that the actin cytoskeleton stiffness can serve as a reliable marker for grading the metastasis of adherent carcinoma cells due to their cytoskeleton change and potentially predicting the migration direction of the cells.
Persistent Identifierhttp://hdl.handle.net/10722/260471
ISSN
2017 Impact Factor: 2.431
2015 SCImago Journal Rankings: 1.233

 

DC FieldValueLanguage
dc.contributor.authorZhou, Z-
dc.contributor.authorSun, X-
dc.contributor.authorMa, J-
dc.contributor.authorTong, M-
dc.contributor.authorTo, KY-
dc.contributor.authorWong, AST-
dc.contributor.authorNgan, AHW-
dc.date.accessioned2018-09-14T08:42:17Z-
dc.date.available2018-09-14T08:42:17Z-
dc.date.issued2017-
dc.identifier.citationJournal of Biomechanics, 2017, v. 60, p. 219-226-
dc.identifier.issn0021-9290-
dc.identifier.urihttp://hdl.handle.net/10722/260471-
dc.description.abstractRecent studies have indicated that the nanoindentation measured stiffness of carcinoma adherent cells is in general lower than normal cells, thus suggesting that cell stiffness may serve as a bio-marker for carcinoma. However, the proper establishment of such a conclusion would require biophysical understanding of the underlying mechanism of the cell stiffness. In this work, we compared the elastic moduli of the actin cytoskeletons of Hey A8 ovarian carcinoma cells with and without metastasis (HM and NM), as measured by 2D atomic force microscopy (AFM) with low-depth nanoindentation via a rate-jump method. The results indicate clearly that HM cells showed lower actin cytoskeleton stiffness atop of their nucleus position and higher actin cytoskeleton stiffness at their rims, compared to NM cells, suggesting that the local stiffness on the cytoskeleton can reflect actin filament distribution. Immunofluorescence staining and scanning electron microscopy (SEM) also indicated that the difference in stiffness in Hey A8 cells with different metastasis is associated with their F-actin rearrangement. Finite-element modelling (FEM) shows that a migrating cell would have its actin filaments bundled together to form stress fibers, which would exhibit lower indentation stiffness than the less aligned arrangement of filaments in a non-migrating cell. The results here indicate that the actin cytoskeleton stiffness can serve as a reliable marker for grading the metastasis of adherent carcinoma cells due to their cytoskeleton change and potentially predicting the migration direction of the cells.-
dc.languageeng-
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/jbiomech-
dc.relation.ispartofJournal of Biomechanics-
dc.subjectAFM indentation-
dc.subjectElastic modulus-
dc.subjectFinite-element modelling-
dc.subjectMetastasis-
dc.subjectOvarian cells-
dc.titleActin cytoskeleton stiffness grades metastatic potential of ovarian carcinoma Hey A8 cells via nanoindentation mapping-
dc.typeArticle-
dc.identifier.emailWong, AST: awong1@hku.hk-
dc.identifier.emailNgan, AHW: hwngan@hku.hk-
dc.identifier.authorityWong, AST=rp00805-
dc.identifier.authorityNgan, AHW=rp00225-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jbiomech.2017.06.040-
dc.identifier.pmid28711162-
dc.identifier.scopuseid_2-s2.0-85023197805-
dc.identifier.hkuros289994-
dc.identifier.volume60-
dc.identifier.spage219-
dc.identifier.epage226-
dc.publisher.placeUnited Kingdom-

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