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Article: Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles

TitleIncorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles
Authors
Keywordsglycan
glycosaminoglycan
polyelectrolyte complex
polysaccharide
Issue Date2016
PublisherDove Medical Press Ltd. The Journal's web site is located at http://www.dovepress.com/articles.php?journal_id=5
Citation
International Journal of Nanomedicine, 2016, v. 11, p. 6149-6159 How to Cite?
AbstractIncorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors examined whether proteins could be directly incorporated into preformed DSCS NPs through a heparin binding domain-mediated interaction. The authors formulated negatively-charged DSCS NPs, and quantified the amount of charged DS in the outer shell of the particles. The authors then mixed the DSCS NPs with heparin-binding proteins (SDF-1α, VEGF, FGF-2, BMP-2, or lysozyme) to achieve incorporation. Data show that for DSCS NPs containing 100 nmol charged glucose sulfate units in DS, up to ~1.5 nmol of monomeric or ~0.75 nmol of dimeric heparin-binding proteins were incorporated without significantly altering the size or zeta potential of the particles. Incorporation efficiencies of these proteins were 95%-100%. In contrast, serum albumin or serum globulin showed minimal incorporation (8% and 4%, respectively) in 50% physiological saline, despite their large adsorption in water (80% and 92%, respectively). The NP-incorporated SDF-1α and VEGF exhibited full activity and sustained thermal stability. An in vivo aerosolization study showed that NP-incorporated SDF-1α persisted in rat lungs for 72 h (~34% remaining), while free SDF-1α was no longer detectable after 16 h. As many growth factors and cytokines contain heparin-binding sites/domains, incorporation into preformed DSCS NPs could facilitate in vivo applications of these proteins.
Persistent Identifierhttp://hdl.handle.net/10722/260281
ISSN
2010 Impact Factor: 4.976
2015 SCImago Journal Rankings: 1.351
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorZaman, P-
dc.contributor.authorWang, JL-
dc.contributor.authorBlau, A-
dc.contributor.authorWang, W-
dc.contributor.authorLi, TN-
dc.contributor.authorKohane, DS-
dc.contributor.authorLoscalzo, J-
dc.contributor.authorZhang, YY-
dc.date.accessioned2018-09-12T07:31:16Z-
dc.date.available2018-09-12T07:31:16Z-
dc.date.issued2016-
dc.identifier.citationInternational Journal of Nanomedicine, 2016, v. 11, p. 6149-6159-
dc.identifier.issn1176-9114-
dc.identifier.urihttp://hdl.handle.net/10722/260281-
dc.description.abstractIncorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors examined whether proteins could be directly incorporated into preformed DSCS NPs through a heparin binding domain-mediated interaction. The authors formulated negatively-charged DSCS NPs, and quantified the amount of charged DS in the outer shell of the particles. The authors then mixed the DSCS NPs with heparin-binding proteins (SDF-1α, VEGF, FGF-2, BMP-2, or lysozyme) to achieve incorporation. Data show that for DSCS NPs containing 100 nmol charged glucose sulfate units in DS, up to ~1.5 nmol of monomeric or ~0.75 nmol of dimeric heparin-binding proteins were incorporated without significantly altering the size or zeta potential of the particles. Incorporation efficiencies of these proteins were 95%-100%. In contrast, serum albumin or serum globulin showed minimal incorporation (8% and 4%, respectively) in 50% physiological saline, despite their large adsorption in water (80% and 92%, respectively). The NP-incorporated SDF-1α and VEGF exhibited full activity and sustained thermal stability. An in vivo aerosolization study showed that NP-incorporated SDF-1α persisted in rat lungs for 72 h (~34% remaining), while free SDF-1α was no longer detectable after 16 h. As many growth factors and cytokines contain heparin-binding sites/domains, incorporation into preformed DSCS NPs could facilitate in vivo applications of these proteins.-
dc.languageeng-
dc.publisherDove Medical Press Ltd. The Journal's web site is located at http://www.dovepress.com/articles.php?journal_id=5-
dc.relation.ispartofInternational Journal of Nanomedicine-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectglycan-
dc.subjectglycosaminoglycan-
dc.subjectpolyelectrolyte complex-
dc.subjectpolysaccharide-
dc.titleIncorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles-
dc.typeArticle-
dc.identifier.emailWang, W: wangwp@hku.hk-
dc.identifier.authorityWang, W=rp02227-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.2147/IJN.S119174-
dc.identifier.pmid27920522-
dc.identifier.pmcidPMC5125769-
dc.identifier.volume11-
dc.identifier.spage6149-
dc.identifier.epage6159-
dc.publisher.placeNew Zealand-

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