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Conference Paper: Kidney infection pathogen/P. gingivalis lipopolysaccharides modulate pro-inflammatory response in hRPTEpiCs
Title | Kidney infection pathogen/P. gingivalis lipopolysaccharides modulate pro-inflammatory response in hRPTEpiCs |
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Authors | |
Issue Date | 2018 |
Publisher | International Association for Dental Research. The Journal's web site is located at http://www.iadr.org/ |
Citation | The 96th General Session and Exhibition of the International Association for Dental Research (IADR) and IADR Pan European Regional (PER) Congress, London, UK, 25-28 July 2018. In Journal of Dental Research, 2018, v. 97 n. Spec Iss B, abstract no. 3492 How to Cite? |
Abstract | Objectives: Renal tubular epithelial cells play a crucial role in kidney function, and it could be markedly affected by kidney infection and increased systemic inflammation. Escherichia coli is one of the most common pathogens for urinary tract infection, while Salmonella species often involve in hematogenous renal infection. Emerging evidence shows that periodontal disease is associated with kidney disease. This pilot study explored the effects of kidney infection-associated bacteria and Porphyromonas gingivalis lipopolysaccharides (LPS) on immuno-inflammatory response in human renal proximal tubular epithelial cells (hRPTEpiCs).
Methods: Primary hRPTEpiCs were obtained from ScienCell (#4100). The cells were treated with LPS respectively from E. coli (ATCC 12014), Salmonella Minnesota (mutant R595) and P. gingivalis (ATCC 33277) at various concentrations (0.1, 1 and 10 µg/ml) as well as IL-1β (0.01, 0.1 and 1 ng/ml) for 24 h. The expression of IL-6 and IL-8 in the culture media was assessed using ELISA kits.
Results: E. coli and S. Minnesota LPS at all concentrations could significantly upregulate the levels of IL-6 and IL-8 in hRPTEpiCs, with reference to the controls (p<0.001). Moreover, IL-1β strongly induced the expression of these inflammatory cytokines as well (p<0.001). However, P. gingivalis LPS did not stimulate notable inflammatory response of these cells.
Conclusions: This preliminary study suggests that E. coli, S. Minnesota and P. gingivalis LPS differentially modulate pro-inflammatory response in hRPTEpiCs. The relevant biological and clinical implications need further investigations. |
Description | Poster Presentation - abstract no. 3492 |
Persistent Identifier | http://hdl.handle.net/10722/259660 |
DC Field | Value | Language |
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dc.contributor.author | Zhao, D | - |
dc.contributor.author | Luo, W | - |
dc.contributor.author | Pelekos, G | - |
dc.contributor.author | Jin, L | - |
dc.date.accessioned | 2018-09-03T04:11:43Z | - |
dc.date.available | 2018-09-03T04:11:43Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | The 96th General Session and Exhibition of the International Association for Dental Research (IADR) and IADR Pan European Regional (PER) Congress, London, UK, 25-28 July 2018. In Journal of Dental Research, 2018, v. 97 n. Spec Iss B, abstract no. 3492 | - |
dc.identifier.uri | http://hdl.handle.net/10722/259660 | - |
dc.description | Poster Presentation - abstract no. 3492 | - |
dc.description.abstract | Objectives: Renal tubular epithelial cells play a crucial role in kidney function, and it could be markedly affected by kidney infection and increased systemic inflammation. Escherichia coli is one of the most common pathogens for urinary tract infection, while Salmonella species often involve in hematogenous renal infection. Emerging evidence shows that periodontal disease is associated with kidney disease. This pilot study explored the effects of kidney infection-associated bacteria and Porphyromonas gingivalis lipopolysaccharides (LPS) on immuno-inflammatory response in human renal proximal tubular epithelial cells (hRPTEpiCs). Methods: Primary hRPTEpiCs were obtained from ScienCell (#4100). The cells were treated with LPS respectively from E. coli (ATCC 12014), Salmonella Minnesota (mutant R595) and P. gingivalis (ATCC 33277) at various concentrations (0.1, 1 and 10 µg/ml) as well as IL-1β (0.01, 0.1 and 1 ng/ml) for 24 h. The expression of IL-6 and IL-8 in the culture media was assessed using ELISA kits. Results: E. coli and S. Minnesota LPS at all concentrations could significantly upregulate the levels of IL-6 and IL-8 in hRPTEpiCs, with reference to the controls (p<0.001). Moreover, IL-1β strongly induced the expression of these inflammatory cytokines as well (p<0.001). However, P. gingivalis LPS did not stimulate notable inflammatory response of these cells. Conclusions: This preliminary study suggests that E. coli, S. Minnesota and P. gingivalis LPS differentially modulate pro-inflammatory response in hRPTEpiCs. The relevant biological and clinical implications need further investigations. | - |
dc.language | eng | - |
dc.publisher | International Association for Dental Research. The Journal's web site is located at http://www.iadr.org/ | - |
dc.relation.ispartof | Journal of Dental Research (Spec Issue) | - |
dc.relation.ispartof | IADR/PER 96th General Session & Exhibition | - |
dc.title | Kidney infection pathogen/P. gingivalis lipopolysaccharides modulate pro-inflammatory response in hRPTEpiCs | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Luo, W: lwhku@hku.hk | - |
dc.identifier.email | Pelekos, G: george74@hku.hk | - |
dc.identifier.email | Jin, L: ljjin@hkucc.hku.hk | - |
dc.identifier.authority | Pelekos, G=rp01894 | - |
dc.identifier.authority | Jin, L=rp00028 | - |
dc.identifier.hkuros | 288117 | - |
dc.identifier.volume | 97 | - |
dc.identifier.issue | Spec Iss B | - |
dc.identifier.spage | abstract no. 3492 | - |
dc.identifier.epage | abstract no. 3492 | - |
dc.publisher.place | United States | - |