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Conference Paper: Chondroitinase ABC induces macrophage polarized to M2 phenotype in central nervous system after injury

TitleChondroitinase ABC induces macrophage polarized to M2 phenotype in central nervous system after injury
Authors
Issue Date2018
PublisherFENS (Federation of European Neuroscience Societies).
Citation
11th FENS Forum of Neuroscience, Berlin, Germany, 7-11 July 2018 How to Cite?
AbstractThe microenvironment of the injured spinal cord activates macrophages/microglia to acquire pro-inflammatory M1 polarization with but transient and few acquiring anti-inflammatory M2 polarization. Following chondroitinase ABC (ChABC) treatment of the injured cord in a rat model, we however found lowered proportion of pro-inflammatory macrophages/microglia. We therefore hypothesized that chondroitin sulfate proteoglycans upregulated in the injured environment influences the balance between M1 and M2 polarization states. To test this, bone marrow collected from adult rats were subjected to in vitro derivation of macrophages and then stimulated with lipopolysaccharide to foster polarisation to the pro-inflammatory state. Following acute treatment of the cultures with ChABC versus vehicle control, RT-PCR analysis for transcripts of polarization markers found lowered expression of such M1 markers as inducible nitric oxide synthase and CD86. In contrast, expression such M2 markers as arginase-1, CCL22 and IL10 were found to be elevated. Experiments with the acutely injured rat model revealed the ChABC-treated injured cord environment increased levels of the IL10 and transforming growth factors beta (TGF-beta) transcripts as compared to the vehicle control. The results add impetus to the development of strategies for timely shift to M2 polarization as a therapeutic goal. [This work is supported by Health and Medical Research Fund, 05163296
DescriptionSession C.10.b Trauma: Spinal cord injury and regeneration - part II - no. C115
Persistent Identifierhttp://hdl.handle.net/10722/259096

 

DC FieldValueLanguage
dc.contributor.authorTam, KW-
dc.contributor.authorShum, DKY-
dc.contributor.authorChan, YS-
dc.date.accessioned2018-09-03T04:01:26Z-
dc.date.available2018-09-03T04:01:26Z-
dc.date.issued2018-
dc.identifier.citation11th FENS Forum of Neuroscience, Berlin, Germany, 7-11 July 2018-
dc.identifier.urihttp://hdl.handle.net/10722/259096-
dc.descriptionSession C.10.b Trauma: Spinal cord injury and regeneration - part II - no. C115 -
dc.description.abstractThe microenvironment of the injured spinal cord activates macrophages/microglia to acquire pro-inflammatory M1 polarization with but transient and few acquiring anti-inflammatory M2 polarization. Following chondroitinase ABC (ChABC) treatment of the injured cord in a rat model, we however found lowered proportion of pro-inflammatory macrophages/microglia. We therefore hypothesized that chondroitin sulfate proteoglycans upregulated in the injured environment influences the balance between M1 and M2 polarization states. To test this, bone marrow collected from adult rats were subjected to in vitro derivation of macrophages and then stimulated with lipopolysaccharide to foster polarisation to the pro-inflammatory state. Following acute treatment of the cultures with ChABC versus vehicle control, RT-PCR analysis for transcripts of polarization markers found lowered expression of such M1 markers as inducible nitric oxide synthase and CD86. In contrast, expression such M2 markers as arginase-1, CCL22 and IL10 were found to be elevated. Experiments with the acutely injured rat model revealed the ChABC-treated injured cord environment increased levels of the IL10 and transforming growth factors beta (TGF-beta) transcripts as compared to the vehicle control. The results add impetus to the development of strategies for timely shift to M2 polarization as a therapeutic goal. [This work is supported by Health and Medical Research Fund, 05163296-
dc.languageeng-
dc.publisherFENS (Federation of European Neuroscience Societies). -
dc.relation.ispartof11th FENS Forum of Neuroscience -
dc.titleChondroitinase ABC induces macrophage polarized to M2 phenotype in central nervous system after injury-
dc.typeConference_Paper-
dc.identifier.emailTam, KW: tamkw@hku.hk-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.authorityShum, DKY=rp00321-
dc.identifier.authorityChan, YS=rp00318-
dc.identifier.hkuros288742-
dc.publisher.placeGermany-

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