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Article: Novel Partitivirus Enhances Virulence Of And Causes Aberrant Gene Expression In Talaromyces Marneffei

TitleNovel Partitivirus Enhances Virulence Of And Causes Aberrant Gene Expression In Talaromyces Marneffei
Authors
KeywordsDimorphic
Fungus
Novel
Partitivirus
Talaromyces marneffei
Issue Date2018
PublisherAmerican Society for Microbiology: Open Access Journals. The Journal's web site is located at http://mbio.asm.org
Citation
mBio, 2018, v. 9, p. e00947-18:1-e00947-18:18 How to Cite?
AbstractTalaromyces marneffei is the most important thermal dimorphic fungus causing systemic mycosis in Southeast Asia. We report the discovery of a novel partitivirus, Talaromyces marneffei partitivirus-1 (TmPV1). TmPV1 was detected in 7 (12.7%) of 55 clinical T. marneffei isolates. Complete genome sequencing of the seven TmPV1 isolates revealed two double-stranded RNA (dsRNA) segments encoding RNA-dependent RNA polymerase (RdRp) and capsid protein, respectively. Phylogenetic analysis showed that TmPV1 occupied a distinct clade among the members of the genus Gammapartitivirus. Transmission electron microscopy confirmed the presence of isometric, nonenveloped viral particles of 30 to 45 nm in diameter, compatible with partitiviruses, in TmPV1-infected T. marneffei. Quantitative reverse transcription-PCR (qRT-PCR) demonstrated higher viral load of TmPV1 in the yeast phase than in the mycelial phase of T. marneffei. Two virus-free isolates, PM1 and PM41, were successfully infected by purified TmPV1 using protoplast transfection. Mice challenged with TmPV1-infected T. marneffei isolates showed significantly shortened survival time (P < 0.0001) and higher fungal burden in organs than mice challenged with isogenic TmPV1-free isolates. Transcriptomic analysis showed that TmPV1 causes aberrant expression of various genes in T. marneffei, with upregulation of potential virulence factors and suppression of RNA interference (RNAi)-related genes. This is the first report of a mycovirus in a thermally dimorphic fungus. Further studies are required to ascertain the mechanism whereby TmPV1 enhances the virulence of T. marneffei in mice and the potential role of RNAi-related genes in antiviral defense in T. marneffei.
Persistent Identifierhttp://hdl.handle.net/10722/258670
ISSN
2019 Impact Factor: 6.784
2015 SCImago Journal Rankings: 3.543
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLau, SKP-
dc.contributor.authorLo, CS-
dc.contributor.authorChow, WN-
dc.contributor.authorFan, RYY-
dc.contributor.authorCai, J-
dc.contributor.authorYuen, KY-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2018-08-22T01:42:11Z-
dc.date.available2018-08-22T01:42:11Z-
dc.date.issued2018-
dc.identifier.citationmBio, 2018, v. 9, p. e00947-18:1-e00947-18:18-
dc.identifier.issn2150-7511-
dc.identifier.urihttp://hdl.handle.net/10722/258670-
dc.description.abstractTalaromyces marneffei is the most important thermal dimorphic fungus causing systemic mycosis in Southeast Asia. We report the discovery of a novel partitivirus, Talaromyces marneffei partitivirus-1 (TmPV1). TmPV1 was detected in 7 (12.7%) of 55 clinical T. marneffei isolates. Complete genome sequencing of the seven TmPV1 isolates revealed two double-stranded RNA (dsRNA) segments encoding RNA-dependent RNA polymerase (RdRp) and capsid protein, respectively. Phylogenetic analysis showed that TmPV1 occupied a distinct clade among the members of the genus Gammapartitivirus. Transmission electron microscopy confirmed the presence of isometric, nonenveloped viral particles of 30 to 45 nm in diameter, compatible with partitiviruses, in TmPV1-infected T. marneffei. Quantitative reverse transcription-PCR (qRT-PCR) demonstrated higher viral load of TmPV1 in the yeast phase than in the mycelial phase of T. marneffei. Two virus-free isolates, PM1 and PM41, were successfully infected by purified TmPV1 using protoplast transfection. Mice challenged with TmPV1-infected T. marneffei isolates showed significantly shortened survival time (P < 0.0001) and higher fungal burden in organs than mice challenged with isogenic TmPV1-free isolates. Transcriptomic analysis showed that TmPV1 causes aberrant expression of various genes in T. marneffei, with upregulation of potential virulence factors and suppression of RNA interference (RNAi)-related genes. This is the first report of a mycovirus in a thermally dimorphic fungus. Further studies are required to ascertain the mechanism whereby TmPV1 enhances the virulence of T. marneffei in mice and the potential role of RNAi-related genes in antiviral defense in T. marneffei.-
dc.languageeng-
dc.publisherAmerican Society for Microbiology: Open Access Journals. The Journal's web site is located at http://mbio.asm.org-
dc.relation.ispartofmBio-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectDimorphic-
dc.subjectFungus-
dc.subjectNovel-
dc.subjectPartitivirus-
dc.subjectTalaromyces marneffei-
dc.titleNovel Partitivirus Enhances Virulence Of And Causes Aberrant Gene Expression In Talaromyces Marneffei-
dc.typeArticle-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailLo, CS: gcslo@connect.hku.hk-
dc.identifier.emailChow, WN: chow5810@HKUCC-COM.hku.hk-
dc.identifier.emailCai, J: caijuice@hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityYuen, KY=rp00366-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1128/mBio.00947-18-
dc.identifier.scopuseid_2-s2.0-85048535609-
dc.identifier.hkuros287299-
dc.identifier.volume9-
dc.identifier.spagee00947-
dc.identifier.epage18:1-
dc.identifier.isiWOS:000454748900020-
dc.publisher.placeUnited States-
dc.identifier.issnl2150-7511-

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