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Article: Efficient RNA drug delivery using red blood cell extracellular vesicles

TitleEfficient RNA drug delivery using red blood cell extracellular vesicles
Authors
Issue Date2018
PublisherNature Publishing Group: Nature Communications. The Journal's web site is located at http://www.nature.com/ncomms/index.html
Citation
Nature Communications, 2018, v. 9 n. 1, p. 2359:1-2359:15 How to Cite?
AbstractMost of the current methods for programmable RNA drug therapies are unsuitable for the clinic due to low uptake efficiency and high cytotoxicity. Extracellular vesicles (EVs) could solve these problems because they represent a natural mode of intercellular communication. However, current cellular sources for EV production are limited in availability and safety in terms of horizontal gene transfer. One potentially ideal source could be human red blood cells (RBCs). Group O-RBCs can be used as universal donors for large-scale EV production since they are readily available in blood banks and they are devoid of DNA. Here, we describe and validate a new strategy to generate large-scale amounts of RBC-derived EVs for the delivery of RNA drugs, including antisense oligonucleotides, Cas9 mRNA, and guide RNAs. RNA drug delivery with RBCEVs shows highly robust microRNA inhibition and CRISPR-Cas9 genome editing in both human cells and xenograft mouse models, with no observable cytotoxicity.
Persistent Identifierhttp://hdl.handle.net/10722/258645
ISSN
2017 Impact Factor: 12.353
2015 SCImago Journal Rankings: 6.539

 

DC FieldValueLanguage
dc.contributor.authorUsman, WM-
dc.contributor.authorPham, TC-
dc.contributor.authorKwok, YY-
dc.contributor.authorVu, LT-
dc.contributor.authorMa, V-
dc.contributor.authorPeng, B-
dc.contributor.authorChan, YS-
dc.contributor.authorWei, L-
dc.contributor.authorChin, SM-
dc.contributor.authorAzad, A-
dc.contributor.authorHe, BA-
dc.contributor.authorLeung, AYH-
dc.contributor.authorYang, M-
dc.contributor.authorShyh-Chang, N-
dc.contributor.authorCho, WC-
dc.contributor.authorShi, J-
dc.contributor.authorLe, MTN-
dc.date.accessioned2018-08-22T01:41:46Z-
dc.date.available2018-08-22T01:41:46Z-
dc.date.issued2018-
dc.identifier.citationNature Communications, 2018, v. 9 n. 1, p. 2359:1-2359:15-
dc.identifier.issn2041-1723-
dc.identifier.urihttp://hdl.handle.net/10722/258645-
dc.description.abstractMost of the current methods for programmable RNA drug therapies are unsuitable for the clinic due to low uptake efficiency and high cytotoxicity. Extracellular vesicles (EVs) could solve these problems because they represent a natural mode of intercellular communication. However, current cellular sources for EV production are limited in availability and safety in terms of horizontal gene transfer. One potentially ideal source could be human red blood cells (RBCs). Group O-RBCs can be used as universal donors for large-scale EV production since they are readily available in blood banks and they are devoid of DNA. Here, we describe and validate a new strategy to generate large-scale amounts of RBC-derived EVs for the delivery of RNA drugs, including antisense oligonucleotides, Cas9 mRNA, and guide RNAs. RNA drug delivery with RBCEVs shows highly robust microRNA inhibition and CRISPR-Cas9 genome editing in both human cells and xenograft mouse models, with no observable cytotoxicity.-
dc.languageeng-
dc.publisherNature Publishing Group: Nature Communications. The Journal's web site is located at http://www.nature.com/ncomms/index.html-
dc.relation.ispartofNature Communications-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleEfficient RNA drug delivery using red blood cell extracellular vesicles-
dc.typeArticle-
dc.identifier.emailHe, BA: alexhe@hku.hk-
dc.identifier.emailLeung, AYH: ayhleung@hku.hk-
dc.identifier.authorityLeung, AYH=rp00265-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41467-018-04791-8-
dc.identifier.hkuros287225-
dc.identifier.volume9-
dc.identifier.issue1-
dc.identifier.spage2359:1-
dc.identifier.epage2359:15-
dc.publisher.placeUnited Kingdom-

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