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Conference Paper: Cloning and characterization of chicken galanin type I receptors

TitleCloning and characterization of chicken galanin type I receptors
Authors
KeywordsChicken
Galanin
Galanin receptor
GPCR
Issue Date2009
PublisherPoultry Science Association Inc. The Journal's web site is located at http://ps.fass.org
Citation
Poultry Science Association (PAS) 98th Annual meeting 2009, Raleigh, North Carolina, USA, 20-23 July 2009. In Poultry Science, 2009, v. 88 n. Suppl. 1, p. 122, abstract no. 417P How to Cite?
AbstractIn the present study, we report the identification of 2 chicken genes with considerable homology to galanin receptor type 1 (GalR1). Galanin, a 29- to 30- amino acid neuropeptide with diverse physiological effects, has been reported to be widely distributed in mammalian nervous systems and peripheral tissues. Through the interactions with the 3 known distinct G protein-coupled receptors (GPCRs), i.e. GalR1, 2 and 3, galanin was found to be involved in a broad spectrum of biological functions including modulation of hormone release, nociception, cognitive and feeding behaviour in mammalian species. To our knowledge, galanin receptors have yet to be cloned and characterized in any avian species. Using reverse-transcription polymerase chain reaction (RTPCR), 2 full-length cDNAs of GalR1 homologues, which we termed GalR1a and GalR1b, were cloned from chicken brain and intestine tissue respectively. GalR1a encodes a 357- amino acid precursor protein that shares 84%, 82% and 84% sequence identities to the human, mouse and rat homologues respectively. On the other hand, GalR1b is 363 amino acids in length with comparatively lower homologies to the mammalian homologues (human, 53%; mouse, 53%; rat, 52%). Using RT-PCR, we also examined the expression of the two receptors in adult chicken tissues. Both GalR1a and GalR1b were found to be expressed in most of the tissues examined with similar patterns. Using a pGL3-CRE-luciferase reporter system, forskolin-stimulated luciferase activity in Chinese hamster ovary (CHO) cells expressing GalR1a was inhibited by galanin in a dose-dependent manner, confirming the functional coupling of Gi protein to GalR1a. The same functional assay is under way for GalR1b. Cloning and characterization of chicken galanin receptors would provide a better insight into the physiological functions of galanin in the avian species.
Persistent Identifierhttp://hdl.handle.net/10722/257931
ISSN
2015 Impact Factor: 1.685
2015 SCImago Journal Rankings: 1.162

 

DC FieldValueLanguage
dc.contributor.authorHo, CWJ-
dc.contributor.authorZhao, D-
dc.contributor.authorKwok, HYA-
dc.contributor.authorWang, Y-
dc.contributor.authorLeung, FCC-
dc.date.accessioned2018-08-17T07:31:43Z-
dc.date.available2018-08-17T07:31:43Z-
dc.date.issued2009-
dc.identifier.citationPoultry Science Association (PAS) 98th Annual meeting 2009, Raleigh, North Carolina, USA, 20-23 July 2009. In Poultry Science, 2009, v. 88 n. Suppl. 1, p. 122, abstract no. 417P-
dc.identifier.issn0032-5791-
dc.identifier.urihttp://hdl.handle.net/10722/257931-
dc.description.abstractIn the present study, we report the identification of 2 chicken genes with considerable homology to galanin receptor type 1 (GalR1). Galanin, a 29- to 30- amino acid neuropeptide with diverse physiological effects, has been reported to be widely distributed in mammalian nervous systems and peripheral tissues. Through the interactions with the 3 known distinct G protein-coupled receptors (GPCRs), i.e. GalR1, 2 and 3, galanin was found to be involved in a broad spectrum of biological functions including modulation of hormone release, nociception, cognitive and feeding behaviour in mammalian species. To our knowledge, galanin receptors have yet to be cloned and characterized in any avian species. Using reverse-transcription polymerase chain reaction (RTPCR), 2 full-length cDNAs of GalR1 homologues, which we termed GalR1a and GalR1b, were cloned from chicken brain and intestine tissue respectively. GalR1a encodes a 357- amino acid precursor protein that shares 84%, 82% and 84% sequence identities to the human, mouse and rat homologues respectively. On the other hand, GalR1b is 363 amino acids in length with comparatively lower homologies to the mammalian homologues (human, 53%; mouse, 53%; rat, 52%). Using RT-PCR, we also examined the expression of the two receptors in adult chicken tissues. Both GalR1a and GalR1b were found to be expressed in most of the tissues examined with similar patterns. Using a pGL3-CRE-luciferase reporter system, forskolin-stimulated luciferase activity in Chinese hamster ovary (CHO) cells expressing GalR1a was inhibited by galanin in a dose-dependent manner, confirming the functional coupling of Gi protein to GalR1a. The same functional assay is under way for GalR1b. Cloning and characterization of chicken galanin receptors would provide a better insight into the physiological functions of galanin in the avian species.-
dc.languageeng-
dc.publisherPoultry Science Association Inc. The Journal's web site is located at http://ps.fass.org-
dc.relation.ispartofPoultry Science-
dc.relation.ispartofPoultry Science Association 98th Annual meeting 2009-
dc.subjectChicken-
dc.subjectGalanin-
dc.subjectGalanin receptor-
dc.subjectGPCR-
dc.titleCloning and characterization of chicken galanin type I receptors-
dc.typeConference_Paper-
dc.identifier.emailWang, Y: cdwyj@yahoo.com-
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hk-
dc.identifier.authorityWang, Y=rp00801-
dc.identifier.authorityLeung, FCC=rp00731-
dc.identifier.hkuros182575-
dc.identifier.volume88-
dc.identifier.issueSuppl. 1-
dc.identifier.spage122-
dc.identifier.epage122-
dc.publisher.placeUnited States-

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