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Article: Time-resolved single-step protease activity quantification using nanoplasmonic resonator sensors

TitleTime-resolved single-step protease activity quantification using nanoplasmonic resonator sensors
Authors
KeywordsPlasmonic resonator
Surface-enhanced raman scattering
Sensing
Protease
Prostate cancer
Issue Date2010
Citation
ACS Nano, 2010, v. 4, n. 2, p. 978-984 How to Cite?
AbstractProtease activity measurement has broad application in drug screening, diagnosis and disease staging, and molecular profiling. However, conventional immunopeptidemetric assays (IMPA) exhibit low fluorescence signal-to-noise ratios, preventing reliable measurements at lower concentrations in the clinically important picomolar to nanomolar range. Here, we demonstrated a highly sensitive measurement of protease activity using a nanoplasmonic resonator (NPR). NPRs enhance Raman signals by 6.1 × 1010times in a highly reproducible manner, enabling fast detection of proteolytically active prostate-specific antigen (paPSA) activities in real-time, at a sensitivity level of 6 pM (0.2 ng/mL) with a dynamic range of 3 orders of magnitude. Experiments on extracellular fluid (ECF) from the paPSA-positive cells demonstrate specific detection in a complex biofluid background. This method offers a fast, sensitive, accurate, and one-step approach to detect the proteases' activities in very small sample volumes. © 2010 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/257315
ISSN
2017 Impact Factor: 13.709
2015 SCImago Journal Rankings: 7.120
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSun, Cheng-
dc.contributor.authorSu, Kai Hung-
dc.contributor.authorValentine, Jason-
dc.contributor.authorRosa-Bauza, Yazmin T.-
dc.contributor.authorEllman, Jonathan A.-
dc.contributor.authorElboudwarej, Omeed-
dc.contributor.authorMukherjee, Bipasha-
dc.contributor.authorCraik, Charles S.-
dc.contributor.authorShuman, Marc A.-
dc.contributor.authorChen, Fanqing Frank-
dc.contributor.authorZhang, Xiang-
dc.date.accessioned2018-07-24T08:59:27Z-
dc.date.available2018-07-24T08:59:27Z-
dc.date.issued2010-
dc.identifier.citationACS Nano, 2010, v. 4, n. 2, p. 978-984-
dc.identifier.issn1936-0851-
dc.identifier.urihttp://hdl.handle.net/10722/257315-
dc.description.abstractProtease activity measurement has broad application in drug screening, diagnosis and disease staging, and molecular profiling. However, conventional immunopeptidemetric assays (IMPA) exhibit low fluorescence signal-to-noise ratios, preventing reliable measurements at lower concentrations in the clinically important picomolar to nanomolar range. Here, we demonstrated a highly sensitive measurement of protease activity using a nanoplasmonic resonator (NPR). NPRs enhance Raman signals by 6.1 × 1010times in a highly reproducible manner, enabling fast detection of proteolytically active prostate-specific antigen (paPSA) activities in real-time, at a sensitivity level of 6 pM (0.2 ng/mL) with a dynamic range of 3 orders of magnitude. Experiments on extracellular fluid (ECF) from the paPSA-positive cells demonstrate specific detection in a complex biofluid background. This method offers a fast, sensitive, accurate, and one-step approach to detect the proteases' activities in very small sample volumes. © 2010 American Chemical Society.-
dc.languageeng-
dc.relation.ispartofACS Nano-
dc.subjectPlasmonic resonator-
dc.subjectSurface-enhanced raman scattering-
dc.subjectSensing-
dc.subjectProtease-
dc.subjectProstate cancer-
dc.titleTime-resolved single-step protease activity quantification using nanoplasmonic resonator sensors-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1021/nn900757p-
dc.identifier.pmid20121209-
dc.identifier.scopuseid_2-s2.0-77649107246-
dc.identifier.volume4-
dc.identifier.issue2-
dc.identifier.spage978-
dc.identifier.epage984-
dc.identifier.eissn1936-086X-
dc.identifier.isiWOS:000274635800050-

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