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- Publisher Website: 10.1021/nn900757p
- Scopus: eid_2-s2.0-77649107246
- PMID: 20121209
- WOS: WOS:000274635800050
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Article: Time-resolved single-step protease activity quantification using nanoplasmonic resonator sensors
Title | Time-resolved single-step protease activity quantification using nanoplasmonic resonator sensors |
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Authors | |
Keywords | Plasmonic resonator Surface-enhanced raman scattering Sensing Protease Prostate cancer |
Issue Date | 2010 |
Citation | ACS Nano, 2010, v. 4, n. 2, p. 978-984 How to Cite? |
Abstract | Protease activity measurement has broad application in drug screening, diagnosis and disease staging, and molecular profiling. However, conventional immunopeptidemetric assays (IMPA) exhibit low fluorescence signal-to-noise ratios, preventing reliable measurements at lower concentrations in the clinically important picomolar to nanomolar range. Here, we demonstrated a highly sensitive measurement of protease activity using a nanoplasmonic resonator (NPR). NPRs enhance Raman signals by 6.1 × 1010times in a highly reproducible manner, enabling fast detection of proteolytically active prostate-specific antigen (paPSA) activities in real-time, at a sensitivity level of 6 pM (0.2 ng/mL) with a dynamic range of 3 orders of magnitude. Experiments on extracellular fluid (ECF) from the paPSA-positive cells demonstrate specific detection in a complex biofluid background. This method offers a fast, sensitive, accurate, and one-step approach to detect the proteases' activities in very small sample volumes. © 2010 American Chemical Society. |
Persistent Identifier | http://hdl.handle.net/10722/257315 |
ISSN | 2023 Impact Factor: 15.8 2023 SCImago Journal Rankings: 4.593 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Sun, Cheng | - |
dc.contributor.author | Su, Kai Hung | - |
dc.contributor.author | Valentine, Jason | - |
dc.contributor.author | Rosa-Bauza, Yazmin T. | - |
dc.contributor.author | Ellman, Jonathan A. | - |
dc.contributor.author | Elboudwarej, Omeed | - |
dc.contributor.author | Mukherjee, Bipasha | - |
dc.contributor.author | Craik, Charles S. | - |
dc.contributor.author | Shuman, Marc A. | - |
dc.contributor.author | Chen, Fanqing Frank | - |
dc.contributor.author | Zhang, Xiang | - |
dc.date.accessioned | 2018-07-24T08:59:27Z | - |
dc.date.available | 2018-07-24T08:59:27Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | ACS Nano, 2010, v. 4, n. 2, p. 978-984 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | http://hdl.handle.net/10722/257315 | - |
dc.description.abstract | Protease activity measurement has broad application in drug screening, diagnosis and disease staging, and molecular profiling. However, conventional immunopeptidemetric assays (IMPA) exhibit low fluorescence signal-to-noise ratios, preventing reliable measurements at lower concentrations in the clinically important picomolar to nanomolar range. Here, we demonstrated a highly sensitive measurement of protease activity using a nanoplasmonic resonator (NPR). NPRs enhance Raman signals by 6.1 × 1010times in a highly reproducible manner, enabling fast detection of proteolytically active prostate-specific antigen (paPSA) activities in real-time, at a sensitivity level of 6 pM (0.2 ng/mL) with a dynamic range of 3 orders of magnitude. Experiments on extracellular fluid (ECF) from the paPSA-positive cells demonstrate specific detection in a complex biofluid background. This method offers a fast, sensitive, accurate, and one-step approach to detect the proteases' activities in very small sample volumes. © 2010 American Chemical Society. | - |
dc.language | eng | - |
dc.relation.ispartof | ACS Nano | - |
dc.subject | Plasmonic resonator | - |
dc.subject | Surface-enhanced raman scattering | - |
dc.subject | Sensing | - |
dc.subject | Protease | - |
dc.subject | Prostate cancer | - |
dc.title | Time-resolved single-step protease activity quantification using nanoplasmonic resonator sensors | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1021/nn900757p | - |
dc.identifier.pmid | 20121209 | - |
dc.identifier.scopus | eid_2-s2.0-77649107246 | - |
dc.identifier.volume | 4 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 978 | - |
dc.identifier.epage | 984 | - |
dc.identifier.eissn | 1936-086X | - |
dc.identifier.isi | WOS:000274635800050 | - |
dc.identifier.issnl | 1936-0851 | - |