File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Subcellular resolution mapping of endogenous cytokine secretion by nano-plasmonic-resonator sensor array

TitleSubcellular resolution mapping of endogenous cytokine secretion by nano-plasmonic-resonator sensor array
Authors
KeywordsT cell
Biosensing
cytokine
in situ immunoassay
nanoimprint lithography (NIL)
plasmonic resonator
Issue Date2011
Citation
Nano Letters, 2011, v. 11, n. 8, p. 3431-3434 How to Cite?
AbstractLocal extracellular signaling is central for cellular interactions and organizations. We report a novel sensing technique to interrogate extracellular signaling at the subcellular level. We developed an in situ immunoassay based on giant optical enhancement of a tunable nano-plasmonic-resonator array fabricated by nanoimprint lithography. Our nanoplasmonic device significantly increases the signal-to-noise ratio to enable the first time submicrometer resolution quantitative mapping of endogenous cytokine secretion. Our study shows a markedly high local interleukin-2 (IL-2) concentration within the immediate vicinity of the cell which finally validates a decades-old hypothesis on autocrine physiological concentration and spatial range. This general sensing technique can be applied for a broad range of cellular communication studies to improve our understanding of subcellular signaling and function. © 2011 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/257076
ISSN
2017 Impact Factor: 12.08
2015 SCImago Journal Rankings: 9.006
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Sheng-
dc.contributor.authorOta, Sadao-
dc.contributor.authorGuo, Bin-
dc.contributor.authorRyu, Jongeun-
dc.contributor.authorRhodes, Christopher-
dc.contributor.authorXiong, Yi-
dc.contributor.authorKalim, Sheraz-
dc.contributor.authorZeng, Li-
dc.contributor.authorChen, Yong-
dc.contributor.authorTeitell, Michael A.-
dc.contributor.authorZhang, Xiang-
dc.date.accessioned2018-07-24T08:58:46Z-
dc.date.available2018-07-24T08:58:46Z-
dc.date.issued2011-
dc.identifier.citationNano Letters, 2011, v. 11, n. 8, p. 3431-3434-
dc.identifier.issn1530-6984-
dc.identifier.urihttp://hdl.handle.net/10722/257076-
dc.description.abstractLocal extracellular signaling is central for cellular interactions and organizations. We report a novel sensing technique to interrogate extracellular signaling at the subcellular level. We developed an in situ immunoassay based on giant optical enhancement of a tunable nano-plasmonic-resonator array fabricated by nanoimprint lithography. Our nanoplasmonic device significantly increases the signal-to-noise ratio to enable the first time submicrometer resolution quantitative mapping of endogenous cytokine secretion. Our study shows a markedly high local interleukin-2 (IL-2) concentration within the immediate vicinity of the cell which finally validates a decades-old hypothesis on autocrine physiological concentration and spatial range. This general sensing technique can be applied for a broad range of cellular communication studies to improve our understanding of subcellular signaling and function. © 2011 American Chemical Society.-
dc.languageeng-
dc.relation.ispartofNano Letters-
dc.subjectT cell-
dc.subjectBiosensing-
dc.subjectcytokine-
dc.subjectin situ immunoassay-
dc.subjectnanoimprint lithography (NIL)-
dc.subjectplasmonic resonator-
dc.titleSubcellular resolution mapping of endogenous cytokine secretion by nano-plasmonic-resonator sensor array-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1021/nl2018838-
dc.identifier.pmid21780816-
dc.identifier.scopuseid_2-s2.0-80051648753-
dc.identifier.volume11-
dc.identifier.issue8-
dc.identifier.spage3431-
dc.identifier.epage3434-
dc.identifier.eissn1530-6992-
dc.identifier.isiWOS:000293665600064-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats