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- Publisher Website: 10.1038/nmeth.4498
- Scopus: eid_2-s2.0-85036620547
- PMID: 29106405
- WOS: WOS:000416604800026
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Article: Isolation and 3D expansion of multipotent Sox9+mouse lung progenitors
Title | Isolation and 3D expansion of multipotent Sox9<sup>+</sup>mouse lung progenitors |
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Authors | |
Issue Date | 2017 |
Citation | Nature Methods, 2017, v. 14, n. 12, p. 1205-1212 How to Cite? |
Abstract | © 2017 Nature America, Inc., part of Springer Nature. All rights reserved. Multiple adult tissues are maintained by stem cells of restricted developmental potential which can only form a subset of lineages within the tissue. For instance, the two adult lung epithelial compartments (airways and alveoli) are separately maintained by distinct lineage-restricted stem cells. A challenge has been to obtain multipotent stem cells and/or progenitors that can generate all epithelial cell types of a given tissue. Here we show that mouse Sox9 + multipotent embryonic lung progenitors can be isolated and expanded long term in 3D culture. Cultured Sox9 + progenitors transcriptionally resemble their in vivo counterparts and generate both airway and alveolar cell types in vitro. Sox9 + progenitors that were transplanted into injured adult mouse lungs differentiated into all major airway and alveolar lineages in vivo in a region-appropriate fashion. We propose that a single expandable embryonic lung progenitor population with broader developmental competence may eventually be used as an alternative for region-restricted adult tissue stem cells in regenerative medicine. |
Persistent Identifier | http://hdl.handle.net/10722/254484 |
ISSN | 2023 Impact Factor: 36.1 2023 SCImago Journal Rankings: 14.796 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Nichane, Massimo | - |
dc.contributor.author | Javed, Asif | - |
dc.contributor.author | Sivakamasundari, V. | - |
dc.contributor.author | Ganesan, Monisha | - |
dc.contributor.author | Ang, Lay Teng | - |
dc.contributor.author | Kraus, Petra | - |
dc.contributor.author | Lufkin, Thomas | - |
dc.contributor.author | Loh, Kyle M. | - |
dc.contributor.author | Lim, Bing | - |
dc.date.accessioned | 2018-06-19T15:40:41Z | - |
dc.date.available | 2018-06-19T15:40:41Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Nature Methods, 2017, v. 14, n. 12, p. 1205-1212 | - |
dc.identifier.issn | 1548-7091 | - |
dc.identifier.uri | http://hdl.handle.net/10722/254484 | - |
dc.description.abstract | © 2017 Nature America, Inc., part of Springer Nature. All rights reserved. Multiple adult tissues are maintained by stem cells of restricted developmental potential which can only form a subset of lineages within the tissue. For instance, the two adult lung epithelial compartments (airways and alveoli) are separately maintained by distinct lineage-restricted stem cells. A challenge has been to obtain multipotent stem cells and/or progenitors that can generate all epithelial cell types of a given tissue. Here we show that mouse Sox9 + multipotent embryonic lung progenitors can be isolated and expanded long term in 3D culture. Cultured Sox9 + progenitors transcriptionally resemble their in vivo counterparts and generate both airway and alveolar cell types in vitro. Sox9 + progenitors that were transplanted into injured adult mouse lungs differentiated into all major airway and alveolar lineages in vivo in a region-appropriate fashion. We propose that a single expandable embryonic lung progenitor population with broader developmental competence may eventually be used as an alternative for region-restricted adult tissue stem cells in regenerative medicine. | - |
dc.language | eng | - |
dc.relation.ispartof | Nature Methods | - |
dc.title | Isolation and 3D expansion of multipotent Sox9<sup>+</sup>mouse lung progenitors | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1038/nmeth.4498 | - |
dc.identifier.pmid | 29106405 | - |
dc.identifier.scopus | eid_2-s2.0-85036620547 | - |
dc.identifier.hkuros | 294061 | - |
dc.identifier.volume | 14 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 1205 | - |
dc.identifier.epage | 1212 | - |
dc.identifier.eissn | 1548-7105 | - |
dc.identifier.isi | WOS:000416604800026 | - |
dc.identifier.issnl | 1548-7091 | - |