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Article: Isolation and 3D expansion of multipotent Sox9+mouse lung progenitors

TitleIsolation and 3D expansion of multipotent Sox9<sup>+</sup>mouse lung progenitors
Authors
Issue Date2017
Citation
Nature Methods, 2017, v. 14, n. 12, p. 1205-1212 How to Cite?
Abstract© 2017 Nature America, Inc., part of Springer Nature. All rights reserved. Multiple adult tissues are maintained by stem cells of restricted developmental potential which can only form a subset of lineages within the tissue. For instance, the two adult lung epithelial compartments (airways and alveoli) are separately maintained by distinct lineage-restricted stem cells. A challenge has been to obtain multipotent stem cells and/or progenitors that can generate all epithelial cell types of a given tissue. Here we show that mouse Sox9 + multipotent embryonic lung progenitors can be isolated and expanded long term in 3D culture. Cultured Sox9 + progenitors transcriptionally resemble their in vivo counterparts and generate both airway and alveolar cell types in vitro. Sox9 + progenitors that were transplanted into injured adult mouse lungs differentiated into all major airway and alveolar lineages in vivo in a region-appropriate fashion. We propose that a single expandable embryonic lung progenitor population with broader developmental competence may eventually be used as an alternative for region-restricted adult tissue stem cells in regenerative medicine.
Persistent Identifierhttp://hdl.handle.net/10722/254484
ISSN
2015 Impact Factor: 25.328
2015 SCImago Journal Rankings: 21.715

 

DC FieldValueLanguage
dc.contributor.authorNichane, Massimo-
dc.contributor.authorJaved, Asif-
dc.contributor.authorSivakamasundari, V.-
dc.contributor.authorGanesan, Monisha-
dc.contributor.authorAng, Lay Teng-
dc.contributor.authorKraus, Petra-
dc.contributor.authorLufkin, Thomas-
dc.contributor.authorLoh, Kyle M.-
dc.contributor.authorLim, Bing-
dc.date.accessioned2018-06-19T15:40:41Z-
dc.date.available2018-06-19T15:40:41Z-
dc.date.issued2017-
dc.identifier.citationNature Methods, 2017, v. 14, n. 12, p. 1205-1212-
dc.identifier.issn1548-7091-
dc.identifier.urihttp://hdl.handle.net/10722/254484-
dc.description.abstract© 2017 Nature America, Inc., part of Springer Nature. All rights reserved. Multiple adult tissues are maintained by stem cells of restricted developmental potential which can only form a subset of lineages within the tissue. For instance, the two adult lung epithelial compartments (airways and alveoli) are separately maintained by distinct lineage-restricted stem cells. A challenge has been to obtain multipotent stem cells and/or progenitors that can generate all epithelial cell types of a given tissue. Here we show that mouse Sox9 + multipotent embryonic lung progenitors can be isolated and expanded long term in 3D culture. Cultured Sox9 + progenitors transcriptionally resemble their in vivo counterparts and generate both airway and alveolar cell types in vitro. Sox9 + progenitors that were transplanted into injured adult mouse lungs differentiated into all major airway and alveolar lineages in vivo in a region-appropriate fashion. We propose that a single expandable embryonic lung progenitor population with broader developmental competence may eventually be used as an alternative for region-restricted adult tissue stem cells in regenerative medicine.-
dc.languageeng-
dc.relation.ispartofNature Methods-
dc.titleIsolation and 3D expansion of multipotent Sox9<sup>+</sup>mouse lung progenitors-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1038/nmeth.4498-
dc.identifier.pmid29106405-
dc.identifier.scopuseid_2-s2.0-85036620547-
dc.identifier.volume14-
dc.identifier.issue12-
dc.identifier.spage1205-
dc.identifier.epage1212-
dc.identifier.eissn1548-7105-

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