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Article: Capturing the interactome of newly transcribed RNA

TitleCapturing the interactome of newly transcribed RNA
Authors
Issue Date2018
Citation
Nature Methods, 2018, v. 15, n. 3, p. 213-220 How to Cite?
Abstract© 2018 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RICK will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.
Persistent Identifierhttp://hdl.handle.net/10722/253186
ISSN
2023 Impact Factor: 36.1
2023 SCImago Journal Rankings: 14.796
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBao, Xichen-
dc.contributor.authorGuo, Xiangpeng-
dc.contributor.authorYin, Menghui-
dc.contributor.authorTariq, Muqddas-
dc.contributor.authorLai, Yiwei-
dc.contributor.authorKanwal, Shahzina-
dc.contributor.authorZhou, Jiajian-
dc.contributor.authorLi, Na-
dc.contributor.authorLv, Yuan-
dc.contributor.authorPulido-Quetglas, Carlos-
dc.contributor.authorWang, Xiwei-
dc.contributor.authorJi, Lu-
dc.contributor.authorKhan, Muhammad J.-
dc.contributor.authorZhu, Xihua-
dc.contributor.authorLuo, Zhiwei-
dc.contributor.authorShao, Changwei-
dc.contributor.authorLim, Do Hwan-
dc.contributor.authorLiu, Xiao-
dc.contributor.authorLi, Nan-
dc.contributor.authorWang, Wei-
dc.contributor.authorHe, Minghui-
dc.contributor.authorLiu, Yu Lin-
dc.contributor.authorWard, Carl-
dc.contributor.authorWang, Tong-
dc.contributor.authorZhang, Gong-
dc.contributor.authorWang, Dongye-
dc.contributor.authorYang, Jianhua-
dc.contributor.authorChen, Yiwen-
dc.contributor.authorZhang, Chaolin-
dc.contributor.authorJauch, Ralf-
dc.contributor.authorYang, Yun Gui-
dc.contributor.authorWang, Yangming-
dc.contributor.authorQin, Baoming-
dc.contributor.authorAnko, Minna Liisa-
dc.contributor.authorHutchins, Andrew P.-
dc.contributor.authorSun, Hao-
dc.contributor.authorWang, Huating-
dc.contributor.authorFu, Xiang Dong-
dc.contributor.authorZhang, Biliang-
dc.contributor.authorEsteban, Miguel A.-
dc.date.accessioned2018-05-11T05:38:50Z-
dc.date.available2018-05-11T05:38:50Z-
dc.date.issued2018-
dc.identifier.citationNature Methods, 2018, v. 15, n. 3, p. 213-220-
dc.identifier.issn1548-7091-
dc.identifier.urihttp://hdl.handle.net/10722/253186-
dc.description.abstract© 2018 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RICK will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.-
dc.languageeng-
dc.relation.ispartofNature Methods-
dc.titleCapturing the interactome of newly transcribed RNA-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/nmeth.4595-
dc.identifier.scopuseid_2-s2.0-85042756849-
dc.identifier.volume15-
dc.identifier.issue3-
dc.identifier.spage213-
dc.identifier.epage220-
dc.identifier.eissn1548-7105-
dc.identifier.isiWOS:000426303200018-
dc.identifier.issnl1548-7091-

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