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- Publisher Website: 10.1016/j.freeradbiomed.2018.02.011
- Scopus: eid_2-s2.0-85041708622
- WOS: WOS:000427420600016
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Article: Peroxynitrite enhances self-renewal, proliferation and neuronal differentiation of neural stem/progenitor cells through activating HIF-1α and Wnt/β-catenin signaling pathway
Title | Peroxynitrite enhances self-renewal, proliferation and neuronal differentiation of neural stem/progenitor cells through activating HIF-1α and Wnt/β-catenin signaling pathway |
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Authors | |
Keywords | Hypoxia Neural stem/progenitor cells (NSCs) Neuronal differentiation Peroxynitrite Proliferation Self-renewal |
Issue Date | 2018 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed |
Citation | Free Radical Biology & Medicine, 2018, v. 117, p. 158-167 How to Cite? |
Abstract | Hypoxic/ischemic stimulation could mediate growth and differentiation of neural stem/progenitor cells (NSCs) into mature neurons but its underlying mechanisms are largely unclear. Peroxynitrite formation is considered as a crucial pathological process contributing to cerebral ischemia-reperfusion injury. In the present study, we tested the hypothesis that peroxynitrite at low concentration could function as redox signaling to promote the growth of NSCs under hypoxic/ischemic conditions. Increased NSCs proliferation was accompanied by peroxynitrite production in the rat brains with 1 h of ischemia plus 7 days of reperfusion in vivo. Cell sorting experiments revealed that endogenous peroxynitrite level affected the capacity of proliferation and self-renewal in NSCs in vitro. Hypoxia stimulated peroxynitrite production and promoted NSCs self-renewal, proliferation and neuronal differentiation whereas treatments of peroxynitrite decomposition catalysts (PDCs, FeTMPyP and FeTPPS) blocked the changes in NSCs self-renewal, proliferation and neuronal differentiation. Exogenous peroxynitrite treatment revealed similar effects to promote NSCs proliferation, self-renewal and neuronal differentiation. Furthermore, the neurogenesis-promoting effects of peroxynitrite were partly through activating HIF-1α correlated with enhanced Wnt/β-catenin signaling pathway. In conclusion, peroxynitrite could be a cellular redox signaling for promoting NSCs proliferation, self-renewal and neuronal differentiation and peroxynitrite production could contribute to neurogenesis in ischemic/hypoxic NSCs. |
Persistent Identifier | http://hdl.handle.net/10722/251767 |
ISSN | 2023 Impact Factor: 7.1 2023 SCImago Journal Rankings: 1.752 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chen, X | - |
dc.contributor.author | Zhou, B | - |
dc.contributor.author | Yan, T | - |
dc.contributor.author | Wu, H | - |
dc.contributor.author | Feng, J | - |
dc.contributor.author | Chen, H | - |
dc.contributor.author | GAO, C | - |
dc.contributor.author | Peng, T | - |
dc.contributor.author | Yang, D | - |
dc.contributor.author | Shen, J | - |
dc.date.accessioned | 2018-03-19T07:00:53Z | - |
dc.date.available | 2018-03-19T07:00:53Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Free Radical Biology & Medicine, 2018, v. 117, p. 158-167 | - |
dc.identifier.issn | 0891-5849 | - |
dc.identifier.uri | http://hdl.handle.net/10722/251767 | - |
dc.description.abstract | Hypoxic/ischemic stimulation could mediate growth and differentiation of neural stem/progenitor cells (NSCs) into mature neurons but its underlying mechanisms are largely unclear. Peroxynitrite formation is considered as a crucial pathological process contributing to cerebral ischemia-reperfusion injury. In the present study, we tested the hypothesis that peroxynitrite at low concentration could function as redox signaling to promote the growth of NSCs under hypoxic/ischemic conditions. Increased NSCs proliferation was accompanied by peroxynitrite production in the rat brains with 1 h of ischemia plus 7 days of reperfusion in vivo. Cell sorting experiments revealed that endogenous peroxynitrite level affected the capacity of proliferation and self-renewal in NSCs in vitro. Hypoxia stimulated peroxynitrite production and promoted NSCs self-renewal, proliferation and neuronal differentiation whereas treatments of peroxynitrite decomposition catalysts (PDCs, FeTMPyP and FeTPPS) blocked the changes in NSCs self-renewal, proliferation and neuronal differentiation. Exogenous peroxynitrite treatment revealed similar effects to promote NSCs proliferation, self-renewal and neuronal differentiation. Furthermore, the neurogenesis-promoting effects of peroxynitrite were partly through activating HIF-1α correlated with enhanced Wnt/β-catenin signaling pathway. In conclusion, peroxynitrite could be a cellular redox signaling for promoting NSCs proliferation, self-renewal and neuronal differentiation and peroxynitrite production could contribute to neurogenesis in ischemic/hypoxic NSCs. | - |
dc.language | eng | - |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed | - |
dc.relation.ispartof | Free Radical Biology & Medicine | - |
dc.rights | Posting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | Hypoxia | - |
dc.subject | Neural stem/progenitor cells (NSCs) | - |
dc.subject | Neuronal differentiation | - |
dc.subject | Peroxynitrite | - |
dc.subject | Proliferation | - |
dc.subject | Self-renewal | - |
dc.title | Peroxynitrite enhances self-renewal, proliferation and neuronal differentiation of neural stem/progenitor cells through activating HIF-1α and Wnt/β-catenin signaling pathway | - |
dc.type | Article | - |
dc.identifier.email | Feng, J: fengjh@HKUCC-COM.hku.hk | - |
dc.identifier.email | Chen, H: chenhs@hku.hk | - |
dc.identifier.email | Yang, D: yangdan@hku.hk | - |
dc.identifier.email | Shen, J: shenjg@hku.hk | - |
dc.identifier.authority | Yang, D=rp00825 | - |
dc.identifier.authority | Shen, J=rp00487 | - |
dc.identifier.doi | 10.1016/j.freeradbiomed.2018.02.011 | - |
dc.identifier.scopus | eid_2-s2.0-85041708622 | - |
dc.identifier.hkuros | 284434 | - |
dc.identifier.volume | 117 | - |
dc.identifier.spage | 158 | - |
dc.identifier.epage | 167 | - |
dc.identifier.isi | WOS:000427420600016 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0891-5849 | - |