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Article: Peroxynitrite enhances self-renewal, proliferation and neuronal differentiation of neural stem/progenitor cells through activating HIF-1α and Wnt/β-catenin signaling pathway

TitlePeroxynitrite enhances self-renewal, proliferation and neuronal differentiation of neural stem/progenitor cells through activating HIF-1α and Wnt/β-catenin signaling pathway
Authors
Issue Date2018
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed
Citation
Free Radical Biology & Medicine, 2018, v. 117, p. 158-167 How to Cite?
AbstractHypoxic/ischemic stimulation could mediate growth and differentiation of neural stem/progenitor cells (NSCs) into mature neurons but its underlying mechanisms are largely unclear. Peroxynitrite formation is considered as a crucial pathological process contributing to cerebral ischemia-reperfusion injury. In the present study, we tested the hypothesis that peroxynitrite at low concentration could function as redox signaling to promote the growth of NSCs under hypoxic/ischemic conditions. Increased NSCs proliferation was accompanied by peroxynitrite production in the rat brains with 1 h of ischemia plus 7 days of reperfusion in vivo. Cell sorting experiments revealed that endogenous peroxynitrite level affected the capacity of proliferation and self-renewal in NSCs in vitro. Hypoxia stimulated peroxynitrite production and promoted NSCs self-renewal, proliferation and neuronal differentiation whereas treatments of peroxynitrite decomposition catalysts (PDCs, FeTMPyP and FeTPPS) blocked the changes in NSCs self-renewal, proliferation and neuronal differentiation. Exogenous peroxynitrite treatment revealed similar effects to promote NSCs proliferation, self-renewal and neuronal differentiation. Furthermore, the neurogenesis-promoting effects of peroxynitrite were partly through activating HIF-1α correlated with enhanced Wnt/β-catenin signaling pathway. In conclusion, peroxynitrite could be a cellular redox signaling for promoting NSCs proliferation, self-renewal and neuronal differentiation and peroxynitrite production could contribute to neurogenesis in ischemic/hypoxic NSCs.
Persistent Identifierhttp://hdl.handle.net/10722/251767
ISSN
2017 Impact Factor: 6.02
2015 SCImago Journal Rankings: 2.468
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, X-
dc.contributor.authorZhou, B-
dc.contributor.authorYan, T-
dc.contributor.authorWu, H-
dc.contributor.authorFeng, J-
dc.contributor.authorChen, H-
dc.contributor.authorGAO, C-
dc.contributor.authorPeng, T-
dc.contributor.authorYang, D-
dc.contributor.authorShen, J-
dc.date.accessioned2018-03-19T07:00:53Z-
dc.date.available2018-03-19T07:00:53Z-
dc.date.issued2018-
dc.identifier.citationFree Radical Biology & Medicine, 2018, v. 117, p. 158-167-
dc.identifier.issn0891-5849-
dc.identifier.urihttp://hdl.handle.net/10722/251767-
dc.description.abstractHypoxic/ischemic stimulation could mediate growth and differentiation of neural stem/progenitor cells (NSCs) into mature neurons but its underlying mechanisms are largely unclear. Peroxynitrite formation is considered as a crucial pathological process contributing to cerebral ischemia-reperfusion injury. In the present study, we tested the hypothesis that peroxynitrite at low concentration could function as redox signaling to promote the growth of NSCs under hypoxic/ischemic conditions. Increased NSCs proliferation was accompanied by peroxynitrite production in the rat brains with 1 h of ischemia plus 7 days of reperfusion in vivo. Cell sorting experiments revealed that endogenous peroxynitrite level affected the capacity of proliferation and self-renewal in NSCs in vitro. Hypoxia stimulated peroxynitrite production and promoted NSCs self-renewal, proliferation and neuronal differentiation whereas treatments of peroxynitrite decomposition catalysts (PDCs, FeTMPyP and FeTPPS) blocked the changes in NSCs self-renewal, proliferation and neuronal differentiation. Exogenous peroxynitrite treatment revealed similar effects to promote NSCs proliferation, self-renewal and neuronal differentiation. Furthermore, the neurogenesis-promoting effects of peroxynitrite were partly through activating HIF-1α correlated with enhanced Wnt/β-catenin signaling pathway. In conclusion, peroxynitrite could be a cellular redox signaling for promoting NSCs proliferation, self-renewal and neuronal differentiation and peroxynitrite production could contribute to neurogenesis in ischemic/hypoxic NSCs.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed-
dc.relation.ispartofFree Radical Biology & Medicine-
dc.rightsPosting accepted manuscript (postprint): © <year>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.titlePeroxynitrite enhances self-renewal, proliferation and neuronal differentiation of neural stem/progenitor cells through activating HIF-1α and Wnt/β-catenin signaling pathway-
dc.typeArticle-
dc.identifier.emailFeng, J: fengjh@HKUCC-COM.hku.hk-
dc.identifier.emailChen, H: chenhs@hku.hk-
dc.identifier.emailYang, D: yangdan@hku.hk-
dc.identifier.emailShen, J: shenjg@hku.hk-
dc.identifier.authorityYang, D=rp00825-
dc.identifier.authorityShen, J=rp00487-
dc.identifier.doi10.1016/j.freeradbiomed.2018.02.011-
dc.identifier.hkuros284434-
dc.identifier.volume117-
dc.identifier.spage158-
dc.identifier.epage167-
dc.identifier.isiWOS:000427420600016-
dc.publisher.placeUnited States-

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