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Article: Large-Scale Screens of miRNA-mRNA Interactions Unveiled That the 3'UTR of a Gene Is Targeted by Multiple miRNAs

TitleLarge-Scale Screens of miRNA-mRNA Interactions Unveiled That the 3'UTR of a Gene Is Targeted by Multiple miRNAs
Authors
Issue Date2013
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2013, v. 8, p. 12 How to Cite?
AbstractAnimal microRNA (miRNA) target prediction is still a challenge, although many prediction programs have been exploited. MiRNAs exert their function through partially binding the messenger RNAs (mRNAs; likely at 3' untranslated regions [3'UTRs]), which makes it possible to detect the miRNA-mRNA interactions in vitro by co-transfection of miRNA and a luciferase reporter gene containing the target mRNA fragment into mammalian cells under a dual-luciferase assay system. Here, we constructed a human miRNA expression library and used a dual-luciferase assay system to perform large-scale screens of interactions between miRNAs and the 3'UTRs of seven genes, which included more than 3,000 interactions with triplicate experiments for each interaction. The screening results showed that the 3'UTR of one gene can be targeted by multiple miRNAs. Among the prediction algorithms, a Bayesian phylogenetic miRNA target identification algorithm and a support vector machine (SVM) presented a relatively better performance (27% for EIMMo and 24.7% for miRDB) against the average precision (17.3%) of the nine prediction programs used here. Additionally, we noticed that a relatively high conservation level was shown at the miRNA 3' end targeted regions, as well as the 5' end (seed region) binding sites.
Persistent Identifierhttp://hdl.handle.net/10722/250225
ISSN
2017 Impact Factor: 2.766
2015 SCImago Journal Rankings: 1.395
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhou, P-
dc.contributor.authorXu, W-
dc.contributor.authorPeng, X-
dc.contributor.authorLuo, Z-
dc.contributor.authorXing, Q-
dc.contributor.authorChen, X-
dc.contributor.authorHou, C-
dc.contributor.authorLiang, W-
dc.contributor.authorZhou, J-
dc.contributor.authorWu, X-
dc.contributor.authorZhou, S-
dc.contributor.authorJiang, S-
dc.date.accessioned2017-12-20T09:22:35Z-
dc.date.available2017-12-20T09:22:35Z-
dc.date.issued2013-
dc.identifier.citationPLoS One, 2013, v. 8, p. 12-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/250225-
dc.description.abstractAnimal microRNA (miRNA) target prediction is still a challenge, although many prediction programs have been exploited. MiRNAs exert their function through partially binding the messenger RNAs (mRNAs; likely at 3' untranslated regions [3'UTRs]), which makes it possible to detect the miRNA-mRNA interactions in vitro by co-transfection of miRNA and a luciferase reporter gene containing the target mRNA fragment into mammalian cells under a dual-luciferase assay system. Here, we constructed a human miRNA expression library and used a dual-luciferase assay system to perform large-scale screens of interactions between miRNAs and the 3'UTRs of seven genes, which included more than 3,000 interactions with triplicate experiments for each interaction. The screening results showed that the 3'UTR of one gene can be targeted by multiple miRNAs. Among the prediction algorithms, a Bayesian phylogenetic miRNA target identification algorithm and a support vector machine (SVM) presented a relatively better performance (27% for EIMMo and 24.7% for miRDB) against the average precision (17.3%) of the nine prediction programs used here. Additionally, we noticed that a relatively high conservation level was shown at the miRNA 3' end targeted regions, as well as the 5' end (seed region) binding sites.-
dc.languageeng-
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPLoS One-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleLarge-Scale Screens of miRNA-mRNA Interactions Unveiled That the 3'UTR of a Gene Is Targeted by Multiple miRNAs-
dc.typeArticle-
dc.identifier.emailZhou, P: zhoupeng@hku.hk-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0068204-
dc.identifier.hkuros276945-
dc.identifier.volume8-
dc.identifier.spage12-
dc.identifier.epage12-
dc.identifier.isiWOS:000321736900067-
dc.publisher.placeUnited States-

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