File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Large-scale identification of microRNA targets in murine Dgcr8-deficient embryonic stem cell lines

TitleLarge-scale identification of microRNA targets in murine Dgcr8-deficient embryonic stem cell lines
Authors
Issue Date2012
Citation
PLoS ONE, 2012, v. 7, n. 8 How to Cite?
AbstractSmall RNAs such as microRNAs play important roles in embryonic stem cell maintenance and differentiation. A broad range of microRNAs is expressed in embryonic stem cells while only a fraction of their targets have been identified. We have performed large-scale identification of embryonic stem cell microRNA targets using a murine embryonic stem cell line deficient in the expression of Dgcr8. These cells are heavily depleted for microRNAs, allowing us to reintroduce specific microRNA duplexes and identify refined target sets. We used deep sequencing of small RNAs, mRNA expression profiling and bioinformatics analysis of microRNA seed matches in 3⠲ UTRs to identify target transcripts. Consequently, we have identified a network of microRNAs that converge on the regulation of several important cellular pathways. Additionally, our experiments have revealed a novel candidate for Dgcr8-independent microRNA genesis and highlighted the challenges currently facing miRNA annotation. © 2012 Davis et al.
Persistent Identifierhttp://hdl.handle.net/10722/249068
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDavis, Matthew P A-
dc.contributor.authorAbreu-Goodger, Cei-
dc.contributor.authorvan Dongen, Stijn-
dc.contributor.authorLu, Dong-
dc.contributor.authorTate, Peri H.-
dc.contributor.authorBartonicek, Nenad-
dc.contributor.authorKutter, Claudia-
dc.contributor.authorLiu, Pentao-
dc.contributor.authorSkarnes, William C.-
dc.contributor.authorEnright, Anton J.-
dc.contributor.authorDunham, Ian-
dc.date.accessioned2017-10-27T05:59:01Z-
dc.date.available2017-10-27T05:59:01Z-
dc.date.issued2012-
dc.identifier.citationPLoS ONE, 2012, v. 7, n. 8-
dc.identifier.urihttp://hdl.handle.net/10722/249068-
dc.description.abstractSmall RNAs such as microRNAs play important roles in embryonic stem cell maintenance and differentiation. A broad range of microRNAs is expressed in embryonic stem cells while only a fraction of their targets have been identified. We have performed large-scale identification of embryonic stem cell microRNA targets using a murine embryonic stem cell line deficient in the expression of Dgcr8. These cells are heavily depleted for microRNAs, allowing us to reintroduce specific microRNA duplexes and identify refined target sets. We used deep sequencing of small RNAs, mRNA expression profiling and bioinformatics analysis of microRNA seed matches in 3⠲ UTRs to identify target transcripts. Consequently, we have identified a network of microRNAs that converge on the regulation of several important cellular pathways. Additionally, our experiments have revealed a novel candidate for Dgcr8-independent microRNA genesis and highlighted the challenges currently facing miRNA annotation. © 2012 Davis et al.-
dc.languageeng-
dc.relation.ispartofPLoS ONE-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleLarge-scale identification of microRNA targets in murine Dgcr8-deficient embryonic stem cell lines-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0041762-
dc.identifier.pmid22912678-
dc.identifier.scopuseid_2-s2.0-84865100081-
dc.identifier.volume7-
dc.identifier.issue8-
dc.identifier.spagenull-
dc.identifier.epagenull-
dc.identifier.eissn1932-6203-
dc.identifier.isiWOS:000308063700007-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats