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- Publisher Website: 10.1371/journal.pone.0041762
- Scopus: eid_2-s2.0-84865100081
- PMID: 22912678
- WOS: WOS:000308063700007
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Article: Large-scale identification of microRNA targets in murine Dgcr8-deficient embryonic stem cell lines
Title | Large-scale identification of microRNA targets in murine Dgcr8-deficient embryonic stem cell lines |
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Authors | |
Issue Date | 2012 |
Citation | PLoS ONE, 2012, v. 7, n. 8 How to Cite? |
Abstract | Small RNAs such as microRNAs play important roles in embryonic stem cell maintenance and differentiation. A broad range of microRNAs is expressed in embryonic stem cells while only a fraction of their targets have been identified. We have performed large-scale identification of embryonic stem cell microRNA targets using a murine embryonic stem cell line deficient in the expression of Dgcr8. These cells are heavily depleted for microRNAs, allowing us to reintroduce specific microRNA duplexes and identify refined target sets. We used deep sequencing of small RNAs, mRNA expression profiling and bioinformatics analysis of microRNA seed matches in 3⠲ UTRs to identify target transcripts. Consequently, we have identified a network of microRNAs that converge on the regulation of several important cellular pathways. Additionally, our experiments have revealed a novel candidate for Dgcr8-independent microRNA genesis and highlighted the challenges currently facing miRNA annotation. © 2012 Davis et al. |
Persistent Identifier | http://hdl.handle.net/10722/249068 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Davis, Matthew P A | - |
dc.contributor.author | Abreu-Goodger, Cei | - |
dc.contributor.author | van Dongen, Stijn | - |
dc.contributor.author | Lu, Dong | - |
dc.contributor.author | Tate, Peri H. | - |
dc.contributor.author | Bartonicek, Nenad | - |
dc.contributor.author | Kutter, Claudia | - |
dc.contributor.author | Liu, Pentao | - |
dc.contributor.author | Skarnes, William C. | - |
dc.contributor.author | Enright, Anton J. | - |
dc.contributor.author | Dunham, Ian | - |
dc.date.accessioned | 2017-10-27T05:59:01Z | - |
dc.date.available | 2017-10-27T05:59:01Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | PLoS ONE, 2012, v. 7, n. 8 | - |
dc.identifier.uri | http://hdl.handle.net/10722/249068 | - |
dc.description.abstract | Small RNAs such as microRNAs play important roles in embryonic stem cell maintenance and differentiation. A broad range of microRNAs is expressed in embryonic stem cells while only a fraction of their targets have been identified. We have performed large-scale identification of embryonic stem cell microRNA targets using a murine embryonic stem cell line deficient in the expression of Dgcr8. These cells are heavily depleted for microRNAs, allowing us to reintroduce specific microRNA duplexes and identify refined target sets. We used deep sequencing of small RNAs, mRNA expression profiling and bioinformatics analysis of microRNA seed matches in 3⠲ UTRs to identify target transcripts. Consequently, we have identified a network of microRNAs that converge on the regulation of several important cellular pathways. Additionally, our experiments have revealed a novel candidate for Dgcr8-independent microRNA genesis and highlighted the challenges currently facing miRNA annotation. © 2012 Davis et al. | - |
dc.language | eng | - |
dc.relation.ispartof | PLoS ONE | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Large-scale identification of microRNA targets in murine Dgcr8-deficient embryonic stem cell lines | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1371/journal.pone.0041762 | - |
dc.identifier.pmid | 22912678 | - |
dc.identifier.scopus | eid_2-s2.0-84865100081 | - |
dc.identifier.volume | 7 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | null | - |
dc.identifier.epage | null | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.isi | WOS:000308063700007 | - |
dc.identifier.issnl | 1932-6203 | - |