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Article: PiggyBac transposon mutagenesis: A tool for cancer gene discovery in mice

TitlePiggyBac transposon mutagenesis: A tool for cancer gene discovery in mice
Authors
Issue Date2010
Citation
Science, 2010, v. 330, n. 6007, p. 1104-1107 How to Cite?
AbstractTransposons are mobile DNA segments that can disrupt gene function by inserting in or near genes. Here, we show that insertional mutagenesis by the PiggyBac transposon can be used for cancer gene discovery in mice. PiggyBac transposition in genetically engineered transposon-transposase mice induced cancers whose type (hematopoietic versus solid) and latency were dependent on the regulatory elements introduced into transposons. Analysis of 63 hematopoietic tumors revealed that PiggyBac is capable of genome-wide mutagenesis. The PiggyBac screen uncovered many cancer genes not identified in previous retroviral or Sleeping Beauty transposon screens, including Spic, which encodes a PU.1-related transcription factor, and Hdac7, a histone deacetylase gene. PiggyBac and Sleeping Beauty have different integration preferences. Tomaximize the utility of the tool, we engineered 21 mouse lines to be compatible with both transposon systems in constitutive, tissue- or temporal-specific mutagenesis. Mice with different transposon types, copy numbers, and chromosomal locations support wide applicability.
Persistent Identifierhttp://hdl.handle.net/10722/249044
ISSN
2015 Impact Factor: 34.661
2015 SCImago Journal Rankings: 13.217

 

DC FieldValueLanguage
dc.contributor.authorRad, Roland-
dc.contributor.authorRad, Lena-
dc.contributor.authorWang, Wei-
dc.contributor.authorCadinanos, Juan-
dc.contributor.authorVassiliou, George-
dc.contributor.authorRice, Stephen-
dc.contributor.authorCampos, Lia S.-
dc.contributor.authorYusa, Kosuke-
dc.contributor.authorBanerjee, Ruby-
dc.contributor.authorLi, Meng Amy-
dc.contributor.authorDe La Rosa, Jorge-
dc.contributor.authorStrong, Alexander-
dc.contributor.authorLu, Dong-
dc.contributor.authorEllis, Peter-
dc.contributor.authorConte, Nathalie-
dc.contributor.authorYang, Fang Tang-
dc.contributor.authorLiu, Pentao-
dc.contributor.authorBradley, Allan-
dc.date.accessioned2017-10-27T05:58:57Z-
dc.date.available2017-10-27T05:58:57Z-
dc.date.issued2010-
dc.identifier.citationScience, 2010, v. 330, n. 6007, p. 1104-1107-
dc.identifier.issn0036-8075-
dc.identifier.urihttp://hdl.handle.net/10722/249044-
dc.description.abstractTransposons are mobile DNA segments that can disrupt gene function by inserting in or near genes. Here, we show that insertional mutagenesis by the PiggyBac transposon can be used for cancer gene discovery in mice. PiggyBac transposition in genetically engineered transposon-transposase mice induced cancers whose type (hematopoietic versus solid) and latency were dependent on the regulatory elements introduced into transposons. Analysis of 63 hematopoietic tumors revealed that PiggyBac is capable of genome-wide mutagenesis. The PiggyBac screen uncovered many cancer genes not identified in previous retroviral or Sleeping Beauty transposon screens, including Spic, which encodes a PU.1-related transcription factor, and Hdac7, a histone deacetylase gene. PiggyBac and Sleeping Beauty have different integration preferences. Tomaximize the utility of the tool, we engineered 21 mouse lines to be compatible with both transposon systems in constitutive, tissue- or temporal-specific mutagenesis. Mice with different transposon types, copy numbers, and chromosomal locations support wide applicability.-
dc.languageeng-
dc.relation.ispartofScience-
dc.titlePiggyBac transposon mutagenesis: A tool for cancer gene discovery in mice-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1126/science.1193004-
dc.identifier.pmid20947725-
dc.identifier.scopuseid_2-s2.0-78449294626-
dc.identifier.volume330-
dc.identifier.issue6007-
dc.identifier.spage1104-
dc.identifier.epage1107-
dc.identifier.eissn1095-9203-

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