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Article: Construction of gene-targeting vectors by recombineering

TitleConstruction of gene-targeting vectors by recombineering
Authors
KeywordsE. coli
gene targeting
recombineering
mouse
Conditional knockout
vector
Issue Date2009
Citation
Methods in Molecular Biology, 2009, v. 530, p. 15-27 How to Cite?
AbstractRecombineering is a technology that utilizes the efficient homologous recombination functions encoded by λ phage to manipulate DNA in Escherichia coli. Construction of knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
Persistent Identifierhttp://hdl.handle.net/10722/249031
ISSN
2015 SCImago Journal Rankings: 0.549

 

DC FieldValueLanguage
dc.contributor.authorLee, Song Choon-
dc.contributor.authorWang, Wei-
dc.contributor.authorLiu, Pentao-
dc.date.accessioned2017-10-27T05:58:55Z-
dc.date.available2017-10-27T05:58:55Z-
dc.date.issued2009-
dc.identifier.citationMethods in Molecular Biology, 2009, v. 530, p. 15-27-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/249031-
dc.description.abstractRecombineering is a technology that utilizes the efficient homologous recombination functions encoded by λ phage to manipulate DNA in Escherichia coli. Construction of knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.-
dc.languageeng-
dc.relation.ispartofMethods in Molecular Biology-
dc.subjectE. coli-
dc.subjectgene targeting-
dc.subjectrecombineering-
dc.subjectmouse-
dc.subjectConditional knockout-
dc.subjectvector-
dc.titleConstruction of gene-targeting vectors by recombineering-
dc.typeArticle-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-59745-471-1_2-
dc.identifier.pmid19266337-
dc.identifier.scopuseid_2-s2.0-65549165490-
dc.identifier.volume530-
dc.identifier.spage15-
dc.identifier.epage27-

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