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Conference Paper: Super-resolution imaging in optical scanning holography using structured illumination
Title | Super-resolution imaging in optical scanning holography using structured illumination |
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Authors | |
Keywords | OSH Structured illumination Super-resolution imaging |
Issue Date | 2016 |
Publisher | SPIE - International Society for Optical Engineering. The Journal's web site is located at https://www.spiedigitallibrary.org/conference-proceedings-of-spie |
Citation | SPIE/COS Photonics Asia, Beijing, China, 12-14 October 2016. In Proceedings of SPIE, 2016, v. 10022, article no. 1002203 How to Cite? |
Abstract | As a specific digital holographic microscopy system, optical scanning holography (OSH) is an appealing technique that makes use of the advantages of holography in the application of optical microscopy. In OSH system, a three-dimensional object is scanned with a Fresnel zone plate in a raster fashion, and the electrical signals are demodulated into a complex hologram by heterodyne detection. Then the recorded light wavefront information contained in the hologram allows one to digitally reconstruct the specimen for multiple purposes such as optical sectioning, extended focused imaging as well as three-dimensional imaging. According to Abbe criterion, however, akin to those conventional microscopic imaging systems, OSH suffers from limited resolving power due to the finite sizes of the objective lens and the aperture, i.e., low numerical aperture. To bypass the diffraction barrier in light microscopy, various super-resolution imaging techniques have been proposed. Among those methods, structured illumination is an ensemble imaging concept that modulates the spatial frequency by projecting additional well-defined patterns with different orientation and phase shift onto the specimen. Computational algorithms are then applied to remove the effect of the structure and to reconstruct a super-resolved image beyond the diffraction-limit. In this paper, we introduce this technique in OSH system to scale down the spatial resolution beyond the diffraction limit. The performance of the proposed method is validated by simulation and experimental results. |
Description | Conference 10022 - Holography, Diffractive Optics, and Applications VII |
Persistent Identifier | http://hdl.handle.net/10722/245529 |
ISSN | 2023 SCImago Journal Rankings: 0.152 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ren, Z | - |
dc.contributor.author | Lam, EYM | - |
dc.date.accessioned | 2017-09-18T02:12:18Z | - |
dc.date.available | 2017-09-18T02:12:18Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | SPIE/COS Photonics Asia, Beijing, China, 12-14 October 2016. In Proceedings of SPIE, 2016, v. 10022, article no. 1002203 | - |
dc.identifier.issn | 0277-786X | - |
dc.identifier.uri | http://hdl.handle.net/10722/245529 | - |
dc.description | Conference 10022 - Holography, Diffractive Optics, and Applications VII | - |
dc.description.abstract | As a specific digital holographic microscopy system, optical scanning holography (OSH) is an appealing technique that makes use of the advantages of holography in the application of optical microscopy. In OSH system, a three-dimensional object is scanned with a Fresnel zone plate in a raster fashion, and the electrical signals are demodulated into a complex hologram by heterodyne detection. Then the recorded light wavefront information contained in the hologram allows one to digitally reconstruct the specimen for multiple purposes such as optical sectioning, extended focused imaging as well as three-dimensional imaging. According to Abbe criterion, however, akin to those conventional microscopic imaging systems, OSH suffers from limited resolving power due to the finite sizes of the objective lens and the aperture, i.e., low numerical aperture. To bypass the diffraction barrier in light microscopy, various super-resolution imaging techniques have been proposed. Among those methods, structured illumination is an ensemble imaging concept that modulates the spatial frequency by projecting additional well-defined patterns with different orientation and phase shift onto the specimen. Computational algorithms are then applied to remove the effect of the structure and to reconstruct a super-resolved image beyond the diffraction-limit. In this paper, we introduce this technique in OSH system to scale down the spatial resolution beyond the diffraction limit. The performance of the proposed method is validated by simulation and experimental results. | - |
dc.language | eng | - |
dc.publisher | SPIE - International Society for Optical Engineering. The Journal's web site is located at https://www.spiedigitallibrary.org/conference-proceedings-of-spie | - |
dc.relation.ispartof | Proceedings of SPIE | - |
dc.rights | Copyright 2016 Society of Photo‑Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, and modification of the contents of the publication are prohibited. This article is available online at https://doi.org/10.1117/12.2247791 | - |
dc.subject | OSH | - |
dc.subject | Structured illumination | - |
dc.subject | Super-resolution imaging | - |
dc.title | Super-resolution imaging in optical scanning holography using structured illumination | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Lam, EYM: elam@eee.hku.hk | - |
dc.identifier.authority | Lam, EYM=rp00131 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1117/12.2247791 | - |
dc.identifier.scopus | eid_2-s2.0-85014660268 | - |
dc.identifier.hkuros | 277489 | - |
dc.identifier.volume | 10022 | - |
dc.identifier.spage | article no. 1002203 | - |
dc.identifier.epage | article no. 1002203 | - |
dc.identifier.isi | WOS:000399000000002 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0277-786X | - |