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Article: Elevated homocysteine in human abdominal aortic aneurysmal tissues

TitleElevated homocysteine in human abdominal aortic aneurysmal tissues
Authors
Keywordsabdominal aortic aneurysm (AAA)
genetics
homocysteine
methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism
vascular smooth muscle cells
Issue Date2017
PublisherSage Publications Ltd.
Citation
Vascular Medicine, 2017, v. 22 n. 5, p. 370-377 How to Cite?
AbstractAn abnormally high level of homocysteine (Hcy) has been consistently observed in the blood of abdominal aortic aneurysm (AAA) patients. However, the expression of Hcy in human AAA tissues has not been investigated. In this study, the expression of Hcy in aneurysmal tissues from AAA patients (n=30) was compared with non-aneurysmal tissues from organ donors (n=31) by dot blotting and immunohistochemistry. A significantly higher expression of Hcy was observed in AAA than control tissues (p<0.001). Furthermore, the associations of methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism, detected by polymerase chain reaction-restriction fragment length polymorphism, with both AAA and tissue Hcy expression were evaluated. Our results showed MTHFR C677T polymorphism was not significantly associated with AAA or tissue Hcy expression. Lastly, the expression of Hcy in vascular smooth muscle cells (VSMCs), which were isolated from human aortic tissues by explant culture, and their release to cultured media was investigated by dot blotting. The AAA VSMCs expressed and released a significantly higher level of Hcy than the control VSMCs (p<0.001). In summary, our novel findings showed Hcy expression was abnormally elevated in human AAA tissues, which may not be dependent on MTHFR C677T polymorphism. © The Author(s) 2017.
Persistent Identifierhttp://hdl.handle.net/10722/245321
ISSN
2017 Impact Factor: 2.393
2015 SCImago Journal Rankings: 0.703

 

DC FieldValueLanguage
dc.contributor.authorChan, YTC-
dc.contributor.authorCheng, SWK-
dc.date.accessioned2017-09-18T02:08:33Z-
dc.date.available2017-09-18T02:08:33Z-
dc.date.issued2017-
dc.identifier.citationVascular Medicine, 2017, v. 22 n. 5, p. 370-377-
dc.identifier.issn1358-863X-
dc.identifier.urihttp://hdl.handle.net/10722/245321-
dc.description.abstractAn abnormally high level of homocysteine (Hcy) has been consistently observed in the blood of abdominal aortic aneurysm (AAA) patients. However, the expression of Hcy in human AAA tissues has not been investigated. In this study, the expression of Hcy in aneurysmal tissues from AAA patients (n=30) was compared with non-aneurysmal tissues from organ donors (n=31) by dot blotting and immunohistochemistry. A significantly higher expression of Hcy was observed in AAA than control tissues (p<0.001). Furthermore, the associations of methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism, detected by polymerase chain reaction-restriction fragment length polymorphism, with both AAA and tissue Hcy expression were evaluated. Our results showed MTHFR C677T polymorphism was not significantly associated with AAA or tissue Hcy expression. Lastly, the expression of Hcy in vascular smooth muscle cells (VSMCs), which were isolated from human aortic tissues by explant culture, and their release to cultured media was investigated by dot blotting. The AAA VSMCs expressed and released a significantly higher level of Hcy than the control VSMCs (p<0.001). In summary, our novel findings showed Hcy expression was abnormally elevated in human AAA tissues, which may not be dependent on MTHFR C677T polymorphism. © The Author(s) 2017.-
dc.languageeng-
dc.publisherSage Publications Ltd.-
dc.relation.ispartofVascular Medicine-
dc.rightsVascular Medicine. Copyright © Sage Publications Ltd.-
dc.subjectabdominal aortic aneurysm (AAA)-
dc.subjectgenetics-
dc.subjecthomocysteine-
dc.subjectmethylenetetrahydrofolate reductase (MTHFR) C677T polymorphism-
dc.subjectvascular smooth muscle cells-
dc.titleElevated homocysteine in human abdominal aortic aneurysmal tissues-
dc.typeArticle-
dc.identifier.emailChan, YTC: cytc@hku.hk-
dc.identifier.emailCheng, SWK: swkcheng@hku.hk-
dc.identifier.authorityCheng, SWK=rp00374-
dc.identifier.doi10.1177/1358863X17718260-
dc.identifier.pmid28709390-
dc.identifier.scopuseid_2-s2.0-85031284945-
dc.identifier.hkuros278324-
dc.identifier.volume22-
dc.identifier.issue5-
dc.identifier.spage370-
dc.identifier.epage377-
dc.publisher.placeUnited Kingdom-

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