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Article: A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation

TitleA Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation
Authors
Issue Date2017
PublisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651
Citation
Methods in Molecular Biology, 2017, v. 1633, p. 193-218 How to Cite?
AbstractThe recent advent of next-generation sequencing (NGS) has greatly accelerated identification of gene mutations in myeloid malignancies at unprecedented speed that will soon outpace their functional validation by conventional laboratory techniques and animal models. A high-throughput whole-organism model is useful for the functional validation of new mutations. We recently reported the use of zebrafish to evaluate the hematopoietic function of isocitrate dehydrogenase 1 (IDH1) and the effects of expressing human IDH1-R132H that is frequently identified in human acute myeloid leukemia (AML), in myelopoiesis, with a view to develop zebrafish as a model of AML. Here, we use IDH1 as an example to describe a comprehensive approach to evaluate hematopoietic gene function and the effects of mutations using zebrafish as a model.
Persistent Identifierhttp://hdl.handle.net/10722/245108
ISSN
2015 SCImago Journal Rankings: 0.549

 

DC FieldValueLanguage
dc.contributor.authorMa, ACH-
dc.contributor.authorShi, X-
dc.contributor.authorHe, BA-
dc.contributor.authorGuo, Y-
dc.contributor.authorLeung, AYH-
dc.date.accessioned2017-09-18T02:04:45Z-
dc.date.available2017-09-18T02:04:45Z-
dc.date.issued2017-
dc.identifier.citationMethods in Molecular Biology, 2017, v. 1633, p. 193-218-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/245108-
dc.description.abstractThe recent advent of next-generation sequencing (NGS) has greatly accelerated identification of gene mutations in myeloid malignancies at unprecedented speed that will soon outpace their functional validation by conventional laboratory techniques and animal models. A high-throughput whole-organism model is useful for the functional validation of new mutations. We recently reported the use of zebrafish to evaluate the hematopoietic function of isocitrate dehydrogenase 1 (IDH1) and the effects of expressing human IDH1-R132H that is frequently identified in human acute myeloid leukemia (AML), in myelopoiesis, with a view to develop zebrafish as a model of AML. Here, we use IDH1 as an example to describe a comprehensive approach to evaluate hematopoietic gene function and the effects of mutations using zebrafish as a model.-
dc.languageeng-
dc.publisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651-
dc.relation.ispartofMethods in Molecular Biology-
dc.rightsThe final publication is available at Springer via http://dx.doi.org/[insert DOI]-
dc.titleA Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation-
dc.typeArticle-
dc.identifier.emailHe, BA: alexhe@hku.hk-
dc.identifier.emailLeung, AYH: ayhleung@hku.hk-
dc.identifier.authorityLeung, AYH=rp00265-
dc.identifier.doi10.1007/978-1-4939-7142-8_13-
dc.identifier.pmid28735489-
dc.identifier.hkuros275924-
dc.identifier.volume1633-
dc.identifier.spage193-
dc.identifier.epage218-
dc.publisher.placeUnited States-

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