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- Publisher Website: 10.1208/s12248-012-9444-4
- Scopus: eid_2-s2.0-84877031136
- PMID: 23229335
- WOS: WOS:000317136100004
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Article: Highly stabilized curcumin nanoparticles tested in an in vitro blood-brain barrier model and in alzheimer's disease Tg2576 Mice
Title | Highly stabilized curcumin nanoparticles tested in an in vitro blood-brain barrier model and in alzheimer's disease Tg2576 Mice |
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Authors | |
Keywords | pharmacokinetic Alzheimer's disease behavior tests nanocurcumin oral route |
Issue Date | 2013 |
Citation | AAPS Journal, 2013, v. 15, n. 2, p. 324-336 How to Cite? |
Abstract | The therapeutic effects of curcumin in treating Alzheimer's disease (AD) depend on the ability to penetrate the blood-brain barrier. The latest nanoparticle technology can help to improve the bioavailability of curcumin, which is affected by the final particle size and stability. We developed a stable curcumin nanoparticle formulation to test in vitro and in AD model Tg2576 mice. Flash nanoprecipitation of curcumin, polyethylene glycol-polylactic acid co-block polymer, and polyvinylpyrrolidone in a multi-inlet vortex mixer, followed by freeze drying with β-cyclodextrin, produced dry nanocurcumin with mean particle size < 80 nm. Nanocurcumin powder, unformulated curcumin, or placebo was orally administered to Tg2576 mice for 3 months. Before and after treatment, memory was measured by radial arm maze and contextual fear conditioning tests. Nanocurcumin produced significantly (p = 0.04) better cue memory in the contextual fear conditioning test than placebo and tendencies toward better working memory in the radial arm maze test than ordinary curcumin (p = 0.14) or placebo (p = 0.12). Amyloid plaque density, pharmacokinetics, and Madin-Darby canine kidney cell monolayer penetration were measured to further understand in vivo and in vitro mechanisms. Nanocurcumin produced significantly higher curcumin concentration in plasma and six times higher area under the curve and mean residence time in brain than ordinary curcumin. The P app of curcumin and tetrahydrocurcumin were 1.8 à 10 -6 and 1.6 à 10 -5 cm/s, respectively, for nanocurcumin. Our novel nanocurcumin formulation produced highly stabilized nanoparticles with positive treatment effects in Tg2576 mice. © 2012 American Association of Pharmaceutical Scientists. |
Persistent Identifier | http://hdl.handle.net/10722/244147 |
ISSN | 2021 Impact Factor: 3.603 2020 SCImago Journal Rankings: 1.109 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Cheng, Kwok Kin | - |
dc.contributor.author | Yeung, Chin Fung | - |
dc.contributor.author | Ho, Shuk Wai | - |
dc.contributor.author | Chow, Shing Fung | - |
dc.contributor.author | Chow, Albert H L | - |
dc.contributor.author | Baum, Larry | - |
dc.date.accessioned | 2017-08-31T08:56:11Z | - |
dc.date.available | 2017-08-31T08:56:11Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | AAPS Journal, 2013, v. 15, n. 2, p. 324-336 | - |
dc.identifier.issn | 1550-7416 | - |
dc.identifier.uri | http://hdl.handle.net/10722/244147 | - |
dc.description.abstract | The therapeutic effects of curcumin in treating Alzheimer's disease (AD) depend on the ability to penetrate the blood-brain barrier. The latest nanoparticle technology can help to improve the bioavailability of curcumin, which is affected by the final particle size and stability. We developed a stable curcumin nanoparticle formulation to test in vitro and in AD model Tg2576 mice. Flash nanoprecipitation of curcumin, polyethylene glycol-polylactic acid co-block polymer, and polyvinylpyrrolidone in a multi-inlet vortex mixer, followed by freeze drying with β-cyclodextrin, produced dry nanocurcumin with mean particle size < 80 nm. Nanocurcumin powder, unformulated curcumin, or placebo was orally administered to Tg2576 mice for 3 months. Before and after treatment, memory was measured by radial arm maze and contextual fear conditioning tests. Nanocurcumin produced significantly (p = 0.04) better cue memory in the contextual fear conditioning test than placebo and tendencies toward better working memory in the radial arm maze test than ordinary curcumin (p = 0.14) or placebo (p = 0.12). Amyloid plaque density, pharmacokinetics, and Madin-Darby canine kidney cell monolayer penetration were measured to further understand in vivo and in vitro mechanisms. Nanocurcumin produced significantly higher curcumin concentration in plasma and six times higher area under the curve and mean residence time in brain than ordinary curcumin. The P app of curcumin and tetrahydrocurcumin were 1.8 à 10 -6 and 1.6 à 10 -5 cm/s, respectively, for nanocurcumin. Our novel nanocurcumin formulation produced highly stabilized nanoparticles with positive treatment effects in Tg2576 mice. © 2012 American Association of Pharmaceutical Scientists. | - |
dc.language | eng | - |
dc.relation.ispartof | AAPS Journal | - |
dc.subject | pharmacokinetic | - |
dc.subject | Alzheimer's disease | - |
dc.subject | behavior tests | - |
dc.subject | nanocurcumin | - |
dc.subject | oral route | - |
dc.title | Highly stabilized curcumin nanoparticles tested in an in vitro blood-brain barrier model and in alzheimer's disease Tg2576 Mice | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1208/s12248-012-9444-4 | - |
dc.identifier.pmid | 23229335 | - |
dc.identifier.scopus | eid_2-s2.0-84877031136 | - |
dc.identifier.volume | 15 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 324 | - |
dc.identifier.epage | 336 | - |
dc.identifier.isi | WOS:000317136100004 | - |
dc.identifier.issnl | 1550-7416 | - |