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Article: Vitamin D and oxidation-induced DNA damage: Is there a connection?
Title | Vitamin D and oxidation-induced DNA damage: Is there a connection? |
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Authors | |
Issue Date | 2016 |
Citation | Mutagenesis, 2016, v. 31, n. 6, p. 655-659 How to Cite? |
Abstract | © 2016 The Author 2016. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. Oxidation-induced damage to DNA can cause mutations, phenotypic changes and apoptosis. Agents that oppose such damage offer potential therapies for disease prevention. Vitamin D administration reportedly lowered DNA damage in type 2 diabetic mice, and higher DNA damage was reported in mononuclear cells of severely asthmatic patients who were vitamin D deficient. We hypothesised that lower vitamin D status associates with higher oxidation-induced DNA damage. Vitamin D deficiency (plasma 25(OH)D < 50 nmol/l) is highly prevalent worldwide, and association with DNA damage has high potential importance and impact in regard to the future health of vitamin D deficient young adults. In this study, oxidation-induced DNA damage in peripheral lymphocytes of 121 young (18-26 years) adults was measured using the formamidopyrimidine DNA glycosylase (FPG)-assisted comet assay. Plasma 25(OH)D was measured by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Correlational analysis was performed between 25(OH)D and DNA damage. Differences in DNA damage across tertiles of 25(OH)D were explored using analysis of variance. DNA damage in those with 25(OH)D < 50 nmol and ⠥50 nmol/l was compared using the unpaired t-test. Mean (SD) DNA damage (as %DNA in comet tail) and plasma 25(OH)D were, respectively, 18.58 (3.39)% and 44.7 (13.03) nmol/l. Most (82/121; 68%) of the s ubjects were deficient in vitamin D (25(OH)D < 50nmol/l). No significant correlation was seen between 25(OH)D and DNA damage (r = -0.0824; P > 0.05). No significant difference was seen across 25(OH)D tertiles: mean (SD) %DNA in comet tail/25(OH)D nmol/l values in lowest, middle and highest tertiles were, respectively, 18.64 (3.30)/31.6 (4.4), 18.90 (3.98)/42.9 (3.5), 18.19 (2.84)/59.9 (8.5), nor across the binary divide: 18.73 (3.63)% in < 50nmol/l group vs. 18.27 (2.84)% in the ⠥50 nmol/l group. No association between vitamin D and oxidation-induced DNA damage was observed, but vitamin D deficiency was highly prevalent in the young adults studied, and we cannot rule out an ameliorative effect of correction of vitamin D deficiency on DNA damage. |
Persistent Identifier | http://hdl.handle.net/10722/244058 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.859 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wang, Erica W. | - |
dc.contributor.author | Collins, Andrew R. | - |
dc.contributor.author | Pang, Marco Y.C. | - |
dc.contributor.author | Siu, Parco P.M. | - |
dc.contributor.author | Lai, Claudia K.Y. | - |
dc.contributor.author | Woo, Jean | - |
dc.contributor.author | Benzie, Iris F.F. | - |
dc.date.accessioned | 2017-08-31T08:55:55Z | - |
dc.date.available | 2017-08-31T08:55:55Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Mutagenesis, 2016, v. 31, n. 6, p. 655-659 | - |
dc.identifier.issn | 0267-8357 | - |
dc.identifier.uri | http://hdl.handle.net/10722/244058 | - |
dc.description.abstract | © 2016 The Author 2016. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. Oxidation-induced damage to DNA can cause mutations, phenotypic changes and apoptosis. Agents that oppose such damage offer potential therapies for disease prevention. Vitamin D administration reportedly lowered DNA damage in type 2 diabetic mice, and higher DNA damage was reported in mononuclear cells of severely asthmatic patients who were vitamin D deficient. We hypothesised that lower vitamin D status associates with higher oxidation-induced DNA damage. Vitamin D deficiency (plasma 25(OH)D < 50 nmol/l) is highly prevalent worldwide, and association with DNA damage has high potential importance and impact in regard to the future health of vitamin D deficient young adults. In this study, oxidation-induced DNA damage in peripheral lymphocytes of 121 young (18-26 years) adults was measured using the formamidopyrimidine DNA glycosylase (FPG)-assisted comet assay. Plasma 25(OH)D was measured by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Correlational analysis was performed between 25(OH)D and DNA damage. Differences in DNA damage across tertiles of 25(OH)D were explored using analysis of variance. DNA damage in those with 25(OH)D < 50 nmol and ⠥50 nmol/l was compared using the unpaired t-test. Mean (SD) DNA damage (as %DNA in comet tail) and plasma 25(OH)D were, respectively, 18.58 (3.39)% and 44.7 (13.03) nmol/l. Most (82/121; 68%) of the s ubjects were deficient in vitamin D (25(OH)D < 50nmol/l). No significant correlation was seen between 25(OH)D and DNA damage (r = -0.0824; P > 0.05). No significant difference was seen across 25(OH)D tertiles: mean (SD) %DNA in comet tail/25(OH)D nmol/l values in lowest, middle and highest tertiles were, respectively, 18.64 (3.30)/31.6 (4.4), 18.90 (3.98)/42.9 (3.5), 18.19 (2.84)/59.9 (8.5), nor across the binary divide: 18.73 (3.63)% in < 50nmol/l group vs. 18.27 (2.84)% in the ⠥50 nmol/l group. No association between vitamin D and oxidation-induced DNA damage was observed, but vitamin D deficiency was highly prevalent in the young adults studied, and we cannot rule out an ameliorative effect of correction of vitamin D deficiency on DNA damage. | - |
dc.language | eng | - |
dc.relation.ispartof | Mutagenesis | - |
dc.title | Vitamin D and oxidation-induced DNA damage: Is there a connection? | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1093/mutage/gew033 | - |
dc.identifier.scopus | eid_2-s2.0-84994476628 | - |
dc.identifier.volume | 31 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 655 | - |
dc.identifier.epage | 659 | - |
dc.identifier.eissn | 1464-3804 | - |
dc.identifier.isi | WOS:000388015700005 | - |
dc.identifier.issnl | 0267-8357 | - |