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Article: Phosphorylation of MITF by AKT affects its downstream targets and causes TP53-dependent cell senescence

TitlePhosphorylation of MITF by AKT affects its downstream targets and causes TP53-dependent cell senescence
Authors
Issue Date2016
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biocel
Citation
The International Journal of Biochemistry & Cell Biology, 2016, v. 80, p. 132-142 How to Cite?
AbstractMicrophthalmia-associated transcription factor (MITF) plays a crucial role in the melanogenesis and proliferation of melanocytes that is dependent on its abundance and modification. Here, we report that epidermal growth factor (EGF) induces senescence and cyclin-dependent kinase inhibitor 1A (CDKN1A) expression that is related to MITF. We found that MITF could bind TP53 to regulate CDKN1A. Furthermore, the interaction between MITF and TP53 is dependent on AKT activity. We found that AKT phosphorylates MITF at S510. Phosphorylated MITF S510 enhances its affinity to TP53 and promotes CDKN1A expression. Meanwhile, the unphosphorylative MITF promotes TYR expression. The levels of p-MITF-S510 are low in 90% human melanoma samples. Thus the level of p-MITF-S510 could be a possible diagnostic marker for melanoma. Our findings reveal a mechanism for regulating MITF functions in response to EGF stimulation and suggest a possible implementation for preventing the over proliferation of melanoma cells.
Persistent Identifierhttp://hdl.handle.net/10722/243568
ISSN
2017 Impact Factor: 3.247
2015 SCImago Journal Rankings: 2.003
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, C-
dc.contributor.authorZhao, L-
dc.contributor.authorSu, Q-
dc.contributor.authorFan, X-
dc.contributor.authorWang, Y-
dc.contributor.authorGao, S-
dc.contributor.authorWang, H-
dc.contributor.authorChen, H-
dc.contributor.authorChan, CB-
dc.contributor.authorLiu, Z-
dc.date.accessioned2017-08-25T02:56:35Z-
dc.date.available2017-08-25T02:56:35Z-
dc.date.issued2016-
dc.identifier.citationThe International Journal of Biochemistry & Cell Biology, 2016, v. 80, p. 132-142-
dc.identifier.issn1357-2725-
dc.identifier.urihttp://hdl.handle.net/10722/243568-
dc.description.abstractMicrophthalmia-associated transcription factor (MITF) plays a crucial role in the melanogenesis and proliferation of melanocytes that is dependent on its abundance and modification. Here, we report that epidermal growth factor (EGF) induces senescence and cyclin-dependent kinase inhibitor 1A (CDKN1A) expression that is related to MITF. We found that MITF could bind TP53 to regulate CDKN1A. Furthermore, the interaction between MITF and TP53 is dependent on AKT activity. We found that AKT phosphorylates MITF at S510. Phosphorylated MITF S510 enhances its affinity to TP53 and promotes CDKN1A expression. Meanwhile, the unphosphorylative MITF promotes TYR expression. The levels of p-MITF-S510 are low in 90% human melanoma samples. Thus the level of p-MITF-S510 could be a possible diagnostic marker for melanoma. Our findings reveal a mechanism for regulating MITF functions in response to EGF stimulation and suggest a possible implementation for preventing the over proliferation of melanoma cells.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biocel-
dc.relation.ispartofThe International Journal of Biochemistry & Cell Biology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titlePhosphorylation of MITF by AKT affects its downstream targets and causes TP53-dependent cell senescence-
dc.typeArticle-
dc.identifier.emailChan, CB: chancb@hku.hk-
dc.identifier.authorityChan, CB=rp02140-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.biocel.2016.09.029-
dc.identifier.hkuros274300-
dc.identifier.volume80-
dc.identifier.spage132-
dc.identifier.epage142-
dc.identifier.isiWOS:000388053400015-
dc.publisher.placeUnited Kingdom-

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