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Article: Revealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach

TitleRevealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach
Authors
Issue Date2016
PublisherNature Publishing Group: Open Access Journals - Option C. The Journal's web site is located at http://www.nature.com/npjbiofilms/
Citation
npj Biofilms and Microbiomes, 2016, v. 2, p. 5:1-7 How to Cite?
AbstractParticle-tracking microrheology is an in situ technique that allows quantification of biofilm material properties. It overcomes the limitations of alternative techniques such as bulk rheology or force spectroscopy by providing data on region specific material properties at any required biofilm location and can be combined with confocal microscopy and associated structural analysis. This article describes single particle tracking microrheology combined with confocal laser scanning microscopy to resolve the biofilm structure in 3 dimensions and calculate the creep compliances locally. Samples were analysed from Pseudomonas fluorescens biofilms that were cultivated over two timescales (24 h and 48 h) and alternate ionic conditions (with and without calcium chloride supplementation). The region-based creep compliance analysis showed that the creep compliance of biofilm void zones is the primary contributor to biofilm mechanical properties, contributing to the overall viscoelastic character.
Persistent Identifierhttp://hdl.handle.net/10722/243549
ISSN
2017 Impact Factor: 4.128
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCao, H-
dc.contributor.authorHabimana, O-
dc.contributor.authorSafari, A-
dc.contributor.authorHeffernan, R-
dc.contributor.authorDai, Y-
dc.contributor.authorCasey, E-
dc.date.accessioned2017-08-25T02:56:19Z-
dc.date.available2017-08-25T02:56:19Z-
dc.date.issued2016-
dc.identifier.citationnpj Biofilms and Microbiomes, 2016, v. 2, p. 5:1-7-
dc.identifier.issn2055-5008-
dc.identifier.urihttp://hdl.handle.net/10722/243549-
dc.description.abstractParticle-tracking microrheology is an in situ technique that allows quantification of biofilm material properties. It overcomes the limitations of alternative techniques such as bulk rheology or force spectroscopy by providing data on region specific material properties at any required biofilm location and can be combined with confocal microscopy and associated structural analysis. This article describes single particle tracking microrheology combined with confocal laser scanning microscopy to resolve the biofilm structure in 3 dimensions and calculate the creep compliances locally. Samples were analysed from Pseudomonas fluorescens biofilms that were cultivated over two timescales (24 h and 48 h) and alternate ionic conditions (with and without calcium chloride supplementation). The region-based creep compliance analysis showed that the creep compliance of biofilm void zones is the primary contributor to biofilm mechanical properties, contributing to the overall viscoelastic character.-
dc.languageeng-
dc.publisherNature Publishing Group: Open Access Journals - Option C. The Journal's web site is located at http://www.nature.com/npjbiofilms/-
dc.relation.ispartofnpj Biofilms and Microbiomes-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleRevealing region-specific biofilm viscoelastic properties by means of a micro-rheological approach-
dc.typeArticle-
dc.identifier.emailHabimana, O: ohabim@hku.hk-
dc.identifier.authorityHabimana, O=rp02169-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41522-016-0005-y-
dc.identifier.hkuros273796-
dc.identifier.volume2-
dc.identifier.spage5:1-
dc.identifier.epage7-
dc.identifier.isiWOS:000419466300005-
dc.publisher.placeUnited Kingdom-

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