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Article: Acyl-CoA-binding protein ACBP1 modulates sterol synthesis during embryogenesis

TitleAcyl-CoA-binding protein ACBP1 modulates sterol synthesis during embryogenesis
Authors
Issue Date2017
PublisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org
Citation
Plant Physiology, 2017, v. 174 n. 3, p. 1420-1435 How to Cite?
AbstractFatty acids (FAs) and sterols are primary metabolites that exert interrelated functions as structural and signaling lipids. Despite their common syntheses from acetyl-coenzyme A, homeostatic cross talk remains enigmatic. Six Arabidopsis (Arabidopsis thaliana) acyl-coenzyme A-binding proteins (ACBPs) are involved in FA metabolism. ACBP1 interacts with PHOSPHOLIPASE Dα1 and regulates phospholipid composition. Here, its specific role in the negative modulation of sterol synthesis during embryogenesis is reported. ACBP1, likely in a liganded state, interacts with STEROL C4-METHYL OXIDASE1-1 (SMO1-1), a rate-limiting enzyme in the sterol pathway. Proembryo abortion in the double mutant indicated that the ACBP1-SMO1-1 interaction is synthetic lethal, corroborating with their strong promoter activities in developing ovules. Gas chromatography-mass spectrometry revealed quantitative and compositional changes in FAs and sterols upon overexpression or mutation of ACBP1 and/or SMO1-1 Aberrant levels of these metabolites may account for the downstream defect in lipid signaling. GLABRA2 (GL2), encoding a phospholipid/sterol-binding homeodomain transcription factor, was up-regulated in developing seeds of acbp1, smo1-1, and ACBP1+/-smo1-1 in comparison with the wild type. Consistent with the corresponding transcriptional alteration of GL2 targets, high-oil, low-mucilage phenotypes of gl2 were phenocopied in ACBP1+/-smo1-1 Thus, ACBP1 appears to modulate the metabolism of two important lipid classes (FAs and sterols) influencing cellular signaling.
Persistent Identifierhttp://hdl.handle.net/10722/242407
ISSN
2017 Impact Factor: 5.949
2015 SCImago Journal Rankings: 3.642
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLung, SC-
dc.contributor.authorLiao, P-
dc.contributor.authorYeung, E-
dc.contributor.authorHsiao, AS-
dc.contributor.authorXue, Y-
dc.contributor.authorChye, ML-
dc.date.accessioned2017-07-24T01:39:24Z-
dc.date.available2017-07-24T01:39:24Z-
dc.date.issued2017-
dc.identifier.citationPlant Physiology, 2017, v. 174 n. 3, p. 1420-1435-
dc.identifier.issn0032-0889-
dc.identifier.urihttp://hdl.handle.net/10722/242407-
dc.description.abstractFatty acids (FAs) and sterols are primary metabolites that exert interrelated functions as structural and signaling lipids. Despite their common syntheses from acetyl-coenzyme A, homeostatic cross talk remains enigmatic. Six Arabidopsis (Arabidopsis thaliana) acyl-coenzyme A-binding proteins (ACBPs) are involved in FA metabolism. ACBP1 interacts with PHOSPHOLIPASE Dα1 and regulates phospholipid composition. Here, its specific role in the negative modulation of sterol synthesis during embryogenesis is reported. ACBP1, likely in a liganded state, interacts with STEROL C4-METHYL OXIDASE1-1 (SMO1-1), a rate-limiting enzyme in the sterol pathway. Proembryo abortion in the double mutant indicated that the ACBP1-SMO1-1 interaction is synthetic lethal, corroborating with their strong promoter activities in developing ovules. Gas chromatography-mass spectrometry revealed quantitative and compositional changes in FAs and sterols upon overexpression or mutation of ACBP1 and/or SMO1-1 Aberrant levels of these metabolites may account for the downstream defect in lipid signaling. GLABRA2 (GL2), encoding a phospholipid/sterol-binding homeodomain transcription factor, was up-regulated in developing seeds of acbp1, smo1-1, and ACBP1+/-smo1-1 in comparison with the wild type. Consistent with the corresponding transcriptional alteration of GL2 targets, high-oil, low-mucilage phenotypes of gl2 were phenocopied in ACBP1+/-smo1-1 Thus, ACBP1 appears to modulate the metabolism of two important lipid classes (FAs and sterols) influencing cellular signaling.-
dc.languageeng-
dc.publisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org-
dc.relation.ispartofPlant Physiology-
dc.titleAcyl-CoA-binding protein ACBP1 modulates sterol synthesis during embryogenesis-
dc.typeArticle-
dc.identifier.emailChye, ML: mlchye@hku.hk-
dc.identifier.authorityChye, ML=rp00687-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1104/pp.17.00412-
dc.identifier.pmid28500265-
dc.identifier.pmcidPMC5490911-
dc.identifier.scopuseid_2-s2.0-85021817030-
dc.identifier.hkuros273236-
dc.identifier.hkuros275183-
dc.identifier.hkuros281663-
dc.identifier.volume174-
dc.identifier.issue3-
dc.identifier.spage1420-
dc.identifier.epage1435-
dc.identifier.isiWOS:000404233000012-
dc.publisher.placeUnited States-

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