File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Appears in Collections:
Conference Paper: Live attenuated Salmonella-based Vaccines delivering SaEsxA by Type III Secretion System Confer Protection against Staphylococcus aureus Infection
Title | Live attenuated Salmonella-based Vaccines delivering SaEsxA by Type III Secretion System Confer Protection against Staphylococcus aureus Infection |
---|---|
Authors | |
Issue Date | 2017 |
Publisher | The University of Hong Kong. |
Citation | 2017 Hong Kong Inter-University Postgraduate Symposium in Biochemical Sciences, The University of Hong Kong, Hong Kong, 16 June 2017 How to Cite? |
Abstract | Staphylococcus cause a wide range of infection diseases in human and animals. The emergence of antibiotic-resistant strains promote novel strategies for prophylactic vaccine development. In this study, live attenuated Salmonella based vaccine against Staphylococcus aureus infection was developed, in which Salmonella Pathogenesis Island-1 Type 3 Secretion System (SPI-1 T3SS) was employed to deliver SaEsxA, one of ESAT-6-like (Early Secreted Antigenic Target-6) virulence factors of S. aureus. Through fusing with the N-terminal secretion and translocation domain of SPI-1 effector SipA, expression and translocation of SaEsxA into the cytosol of macrophages was detected in vitro. Oral administration of Salmonella-SaEsxA vaccine to BalB/c mice significantly induced SaEsxA-specific mucosal and humoral immune responses, detected by ELISA. Besides, Th1- and Th17- biased cellular immune response was also observed by ELISPOT assay. This multifaceted immune responses further increased the survival rate to 50% for vaccinated mice when challenged with S. aureus Newman strain, whereas all mice in control group died. Thus, the newly developed Salmonella based vaccine delivering SaEsxA by SPI-1 T3SS could confer protection against S. aureus infection. This study provides preclinical data supporting the use of Salmonella SPI-1 T3SS to translocate foreign antigens into the cytosol of antigen presenting cells to induce potent immunity against pathogens. |
Description | Poster Presentation: no. P66 |
Persistent Identifier | http://hdl.handle.net/10722/242155 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xu, C | - |
dc.contributor.author | Zhang, B | - |
dc.contributor.author | Lin, Q | - |
dc.contributor.author | Deng, J | - |
dc.contributor.author | Yu, B | - |
dc.contributor.author | Arya, S | - |
dc.contributor.author | Huang, J | - |
dc.date.accessioned | 2017-07-24T01:36:06Z | - |
dc.date.available | 2017-07-24T01:36:06Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | 2017 Hong Kong Inter-University Postgraduate Symposium in Biochemical Sciences, The University of Hong Kong, Hong Kong, 16 June 2017 | - |
dc.identifier.uri | http://hdl.handle.net/10722/242155 | - |
dc.description | Poster Presentation: no. P66 | - |
dc.description.abstract | Staphylococcus cause a wide range of infection diseases in human and animals. The emergence of antibiotic-resistant strains promote novel strategies for prophylactic vaccine development. In this study, live attenuated Salmonella based vaccine against Staphylococcus aureus infection was developed, in which Salmonella Pathogenesis Island-1 Type 3 Secretion System (SPI-1 T3SS) was employed to deliver SaEsxA, one of ESAT-6-like (Early Secreted Antigenic Target-6) virulence factors of S. aureus. Through fusing with the N-terminal secretion and translocation domain of SPI-1 effector SipA, expression and translocation of SaEsxA into the cytosol of macrophages was detected in vitro. Oral administration of Salmonella-SaEsxA vaccine to BalB/c mice significantly induced SaEsxA-specific mucosal and humoral immune responses, detected by ELISA. Besides, Th1- and Th17- biased cellular immune response was also observed by ELISPOT assay. This multifaceted immune responses further increased the survival rate to 50% for vaccinated mice when challenged with S. aureus Newman strain, whereas all mice in control group died. Thus, the newly developed Salmonella based vaccine delivering SaEsxA by SPI-1 T3SS could confer protection against S. aureus infection. This study provides preclinical data supporting the use of Salmonella SPI-1 T3SS to translocate foreign antigens into the cytosol of antigen presenting cells to induce potent immunity against pathogens. | - |
dc.language | eng | - |
dc.publisher | The University of Hong Kong. | - |
dc.relation.ispartof | Hong Kong Inter-University Postgraduate Symposium in Biochemical Sciences, 2017 | - |
dc.title | Live attenuated Salmonella-based Vaccines delivering SaEsxA by Type III Secretion System Confer Protection against Staphylococcus aureus Infection | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Yu, B: ppayubin@hku.hk | - |
dc.identifier.email | Huang, J: jdhuang@hku.hk | - |
dc.identifier.authority | Huang, J=rp00451 | - |
dc.identifier.hkuros | 273095 | - |
dc.publisher.place | Hong Kong | - |