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- Publisher Website: 10.1016/j.antiviral.2012.03.009
- Scopus: eid_2-s2.0-84860297669
- PMID: 22504097
- WOS: WOS:000303692000007
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Article: Polyfunctional CD8 + T cells are associated with the vaccination-induced control of a novel recombinant influenza virus expressing an HCV epitope
Title | Polyfunctional CD8 + T cells are associated with the vaccination-induced control of a novel recombinant influenza virus expressing an HCV epitope |
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Authors | |
Keywords | Liver Lipopeptide vaccination Hepatitis C virus CD8 T cells + |
Issue Date | 2012 |
Citation | Antiviral Research, 2012, v. 94, n. 2, p. 168-178 How to Cite? |
Abstract | In hepatitis C virus (HCV) infection, CD8 + T cell responses have been shown to be important in viral clearance. Examining the efficacy of CD8 + T cell vaccines against HCV has been limited by the lack of an HCV infectious model in mice and the differences between MHC restriction in humans and mice. Using HLA-A2 transgenic HHD mice, we demonstrate that intranasally delivered Pam2Cys-based lipopeptides containing HLA-A2-restricted HCV epitopes can induce polyfunctional CD8 + T cell responses in several organs including the liver. To examine the activity of these responses in an infectious context, we developed a recombinant influenza virus that expresses the NS5B 2594-2602 epitope from non-structural protein 5B of hepatitis C virus (PR8-HCV NS5B). We showed that mice inoculated with a lipopeptide containing the NS5B epitope had reduced viral loads following challenge with the PR8-HCV NS5B virus. This reduction was associated with the induction of NS5B 2594-2602-specific IFN-γ and TNF-α co-producing CD8 + T cells. The T cell receptor usage in the NS5B 2594-2602 response was found to exhibit a Vβ8.1/8.2 bias that was characterized by a narrow repertoire and a common CDR3β motif. This work has identified CD8 + T cell functions induced by lipopeptides that are associated with viral control and demonstrate the potential of lipopeptide-based vaccines as candidates for treatment of HCV infection. © 2012 Elsevier B.V. |
Persistent Identifier | http://hdl.handle.net/10722/241234 |
ISSN | 2021 Impact Factor: 10.103 2020 SCImago Journal Rankings: 2.052 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Tan, Amabel C L | - |
dc.contributor.author | Eriksson, Emily M Y | - |
dc.contributor.author | Kedzierska, Katherine | - |
dc.contributor.author | Deliyannis, Georgia | - |
dc.contributor.author | Valkenburg, Sophie A. | - |
dc.contributor.author | Zeng, Weiguang | - |
dc.contributor.author | Jackson, David C. | - |
dc.date.accessioned | 2017-05-26T03:37:10Z | - |
dc.date.available | 2017-05-26T03:37:10Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Antiviral Research, 2012, v. 94, n. 2, p. 168-178 | - |
dc.identifier.issn | 0166-3542 | - |
dc.identifier.uri | http://hdl.handle.net/10722/241234 | - |
dc.description.abstract | In hepatitis C virus (HCV) infection, CD8 + T cell responses have been shown to be important in viral clearance. Examining the efficacy of CD8 + T cell vaccines against HCV has been limited by the lack of an HCV infectious model in mice and the differences between MHC restriction in humans and mice. Using HLA-A2 transgenic HHD mice, we demonstrate that intranasally delivered Pam2Cys-based lipopeptides containing HLA-A2-restricted HCV epitopes can induce polyfunctional CD8 + T cell responses in several organs including the liver. To examine the activity of these responses in an infectious context, we developed a recombinant influenza virus that expresses the NS5B 2594-2602 epitope from non-structural protein 5B of hepatitis C virus (PR8-HCV NS5B). We showed that mice inoculated with a lipopeptide containing the NS5B epitope had reduced viral loads following challenge with the PR8-HCV NS5B virus. This reduction was associated with the induction of NS5B 2594-2602-specific IFN-γ and TNF-α co-producing CD8 + T cells. The T cell receptor usage in the NS5B 2594-2602 response was found to exhibit a Vβ8.1/8.2 bias that was characterized by a narrow repertoire and a common CDR3β motif. This work has identified CD8 + T cell functions induced by lipopeptides that are associated with viral control and demonstrate the potential of lipopeptide-based vaccines as candidates for treatment of HCV infection. © 2012 Elsevier B.V. | - |
dc.language | eng | - |
dc.relation.ispartof | Antiviral Research | - |
dc.subject | Liver | - |
dc.subject | Lipopeptide vaccination | - |
dc.subject | Hepatitis C virus | - |
dc.subject | CD8 T cells + | - |
dc.title | Polyfunctional CD8 + T cells are associated with the vaccination-induced control of a novel recombinant influenza virus expressing an HCV epitope | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.antiviral.2012.03.009 | - |
dc.identifier.pmid | 22504097 | - |
dc.identifier.scopus | eid_2-s2.0-84860297669 | - |
dc.identifier.volume | 94 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 168 | - |
dc.identifier.epage | 178 | - |
dc.identifier.eissn | 1872-9096 | - |
dc.identifier.isi | WOS:000303692000007 | - |
dc.identifier.issnl | 0166-3542 | - |