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Conference Paper: Determining the Mechanism of CSR-1 RNAi Pathway in Organizing Holocentromere in Caenorhabditis elegans
Title | Determining the Mechanism of CSR-1 RNAi Pathway in Organizing Holocentromere in Caenorhabditis elegans |
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Authors | |
Issue Date | 2016 |
Citation | Gordon Research Conference on Centromere Biology: Structural and Functional Dynamics of the Centromere in Mitosis and Meiosis, West Dover, VT, USA, 24-29 July 2016 How to Cite? |
Abstract | CSR-1 RNAi pathway is required for the holocentromere organization and proper chromosome segregation in Caenorhabditis elegans. It remains obscure how this pathway functionally links to these two events. A previous study showed that germline genes, which are targets of CSR-1 RNAi pathway, are inversely correlated to HCP-3 occupancy on chromatin. We propose that CSR-1 RNAi pathway may restrict HCP-3 localization on the centromere. To study how the CSR-1 RNAi pathway affects the centromere function, we have closely examined the centromeric protein localization and expression followed by RNAi knockdown of the Argonaute CSR-1. By live imaging, we found that upon csr-1 knockdown, embryos have an altered localization of the centromeric histone H3 protein variant, HCP-3. Our imaging and biochemical data also show that csr-1 knockdown elevated both centromeric and cellular level of HCP-3, respectively, but it did not affect the bipolar arrangement of HCP-3 on centromere. We proposed that the increase in HCP-3 level, which is comparable to CENP-A overexpression in other organisms, may lead to merotelic kinetochore attachment and aneuploidy. We will also test if HCP-3 occupancy is reduced after tethering CSR-1 onto an HCP-3-enriched region by ChIP-qPCR. This study will provide insights on how RNAi and non-coding RNA transcripts may function in centromere organization and implicate how centromere are defined. |
Description | Selected for Talk - Non-Coding Elements Involved in Centromere Function |
Persistent Identifier | http://hdl.handle.net/10722/240403 |
DC Field | Value | Language |
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dc.contributor.author | Wong, YYC | - |
dc.contributor.author | Yuen, KWY | - |
dc.date.accessioned | 2017-04-20T04:47:15Z | - |
dc.date.available | 2017-04-20T04:47:15Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Gordon Research Conference on Centromere Biology: Structural and Functional Dynamics of the Centromere in Mitosis and Meiosis, West Dover, VT, USA, 24-29 July 2016 | - |
dc.identifier.uri | http://hdl.handle.net/10722/240403 | - |
dc.description | Selected for Talk - Non-Coding Elements Involved in Centromere Function | - |
dc.description.abstract | CSR-1 RNAi pathway is required for the holocentromere organization and proper chromosome segregation in Caenorhabditis elegans. It remains obscure how this pathway functionally links to these two events. A previous study showed that germline genes, which are targets of CSR-1 RNAi pathway, are inversely correlated to HCP-3 occupancy on chromatin. We propose that CSR-1 RNAi pathway may restrict HCP-3 localization on the centromere. To study how the CSR-1 RNAi pathway affects the centromere function, we have closely examined the centromeric protein localization and expression followed by RNAi knockdown of the Argonaute CSR-1. By live imaging, we found that upon csr-1 knockdown, embryos have an altered localization of the centromeric histone H3 protein variant, HCP-3. Our imaging and biochemical data also show that csr-1 knockdown elevated both centromeric and cellular level of HCP-3, respectively, but it did not affect the bipolar arrangement of HCP-3 on centromere. We proposed that the increase in HCP-3 level, which is comparable to CENP-A overexpression in other organisms, may lead to merotelic kinetochore attachment and aneuploidy. We will also test if HCP-3 occupancy is reduced after tethering CSR-1 onto an HCP-3-enriched region by ChIP-qPCR. This study will provide insights on how RNAi and non-coding RNA transcripts may function in centromere organization and implicate how centromere are defined. | - |
dc.language | eng | - |
dc.relation.ispartof | Gordon Research Conference on Centromere Biology | - |
dc.title | Determining the Mechanism of CSR-1 RNAi Pathway in Organizing Holocentromere in Caenorhabditis elegans | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Yuen, KWY: kwyyuen@hku.hk | - |
dc.identifier.authority | Yuen, KWY=rp01512 | - |
dc.identifier.hkuros | 268851 | - |
dc.publisher.place | West Dover, VT, USA | - |