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Article: Visualization and quantification of browning using a Ucp1-2A-luciferase knock-in mouse model

TitleVisualization and quantification of browning using a Ucp1-2A-luciferase knock-in mouse model
Authors
Issue Date2017
Citation
Diabetes, 2017, v. 66, n. 2, p. 407-417 How to Cite?
Abstract© 2017 by the American Diabetes Association.Both mammals and adult humans possess classic brown adipocytes and beige adipocytes, and the amount and activity of these adipocytes are considered key factors in combating obesity and its associated metabolic diseases. Uncoupling protein 1 (Ucp1) is the functional marker of both brown and beige adipocytes. To facilitate a reliable, easy, and sensitive measurement of Ucp1 expression both in vivo and in vitro, we generated a Ucp1-2A-luciferase knock-in mouse by deleting the stop codon for the mouse Ucp1 gene and replacing it with a 2A peptide. This peptide was followed by the luciferase coding sequence to recapitulate the expression of the Ucp1 gene at the transcriptional and translational levels. With this mouse, we discovered a cold-sensitive brown/beige adipose depot underneath the skin of the ears, which we named uBAT. Because of the sensitivity and high dynamic range of luciferase activity, the Ucp1-2A-luciferase mouse is useful for both in vitro quantitative determination and in vivo visualization of nonshivering thermogenesis. With the use of this model, we identified and characterized axitinib, an oral small-molecule tyrosine kinase inhibitor, as an effective browning agent.
Persistent Identifierhttp://hdl.handle.net/10722/238904
ISSN
2017 Impact Factor: 7.273
2015 SCImago Journal Rankings: 5.185

 

DC FieldValueLanguage
dc.contributor.authorMao, Liufeng-
dc.contributor.authorNie, Baoming-
dc.contributor.authorNie, Tao-
dc.contributor.authorHui, Xiaoyan-
dc.contributor.authorGao, Xuefei-
dc.contributor.authorLin, Xiaoliang-
dc.contributor.authorLiu, Xin-
dc.contributor.authorXu, Yong-
dc.contributor.authorTang, Xiaofeng-
dc.contributor.authorYuan, Ran-
dc.contributor.authorLi, Kuai-
dc.contributor.authorLi, Peng-
dc.contributor.authorDing, Ke-
dc.contributor.authorWang, Yu-
dc.contributor.authorXu, Aimin-
dc.contributor.authorFei, Jian-
dc.contributor.authorHan, Weiping-
dc.contributor.authorLiu, Pentao-
dc.contributor.authorMadsen, Lise-
dc.contributor.authorKristiansen, Karsten-
dc.contributor.authorZhou, Zhiguang-
dc.contributor.authorDing, Sheng-
dc.contributor.authorWu, Donghai-
dc.date.accessioned2017-02-20T03:17:50Z-
dc.date.available2017-02-20T03:17:50Z-
dc.date.issued2017-
dc.identifier.citationDiabetes, 2017, v. 66, n. 2, p. 407-417-
dc.identifier.issn0012-1797-
dc.identifier.urihttp://hdl.handle.net/10722/238904-
dc.description.abstract© 2017 by the American Diabetes Association.Both mammals and adult humans possess classic brown adipocytes and beige adipocytes, and the amount and activity of these adipocytes are considered key factors in combating obesity and its associated metabolic diseases. Uncoupling protein 1 (Ucp1) is the functional marker of both brown and beige adipocytes. To facilitate a reliable, easy, and sensitive measurement of Ucp1 expression both in vivo and in vitro, we generated a Ucp1-2A-luciferase knock-in mouse by deleting the stop codon for the mouse Ucp1 gene and replacing it with a 2A peptide. This peptide was followed by the luciferase coding sequence to recapitulate the expression of the Ucp1 gene at the transcriptional and translational levels. With this mouse, we discovered a cold-sensitive brown/beige adipose depot underneath the skin of the ears, which we named uBAT. Because of the sensitivity and high dynamic range of luciferase activity, the Ucp1-2A-luciferase mouse is useful for both in vitro quantitative determination and in vivo visualization of nonshivering thermogenesis. With the use of this model, we identified and characterized axitinib, an oral small-molecule tyrosine kinase inhibitor, as an effective browning agent.-
dc.languageeng-
dc.relation.ispartofDiabetes-
dc.titleVisualization and quantification of browning using a Ucp1-2A-luciferase knock-in mouse model-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.2337/db16-0343-
dc.identifier.pmid28108609-
dc.identifier.scopuseid_2-s2.0-85011663118-
dc.identifier.hkuros281425-
dc.identifier.volume66-
dc.identifier.issue2-
dc.identifier.spage407-
dc.identifier.epage417-
dc.identifier.eissn1939-327X-

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